Correlation between the DNA methylation and gene expression of IGFBP5 in breast cancer

Abstract

In summary, our results showed that IGFBP5 promoter and exon-I methylation did not have any differences between tumor and adjacent tissues so that IGFBP5 methylation did not change IGFBP5 gene regulation in breast cancer. This is the first study investigating the IGFBP5 gene methylation in breast cancer.

Septin 9 hypermethylation contributes to migration and resistance to drug treatments in colon cancer

“…In particular, multiple CpG-rich promoter regions were extensively studied over the last decade and associated the methylation content with effect on transcription [17,18]. Similar hypermethylation content was also previously identified in promoter regions of septin 9 and implicated for modulating expression in breast cancer cells [19]. In the current study, we observed that septin 9 expressions in tissues correlate with methylation content in the promoter regions in CRC patient samples.…”

Expression levels of resistant genes affect cervical cancer prognosis

“…Subsequently, dissociation curve analysis was performed, and the reaction products were separated by electrophoresis on a 2% agarose gel and stained with ethidium bromide for confirmation of the PCR products. The mRNA expression levels of MDR1, LRP and GSTP1 were expressed as a ratio relative to GAPDH in each sample, the level of genes expression were normalized to that of the internal (GAPDH) and were determined by the 2 -∆∆Cq method (7). Primer pairs were as follows: Forward, 5'-CCC ATC ATT GCA ATA GCA GG-3' and reverse, 5'-TGT TCA AAC TTC TGC TCC TGA-3' for human MDR1; forward, 5'-CCA GAA CCA GGG AGG CAA GA-3' and reverse, 5'-GAG GCG CCC CAC ATA TGC T-3' for human GSTP1; forward, 5'-GTC TTC GGG CCT GAG CTG GTG TCG-3' and reverse, 5'-CTT GGC CGT CTC TTG GGG GTC CTT-3' for human LRP; and forward, 5'-GAA GGT GAA GGT CGG AGT C-3' and reverse, 5'-GAA GAT GGT GAT GGG ATT TC-3' for human GAPDH.…”

Downregulation of insulin-like growth factor binding protein 5 is involved in intervertebral disc degeneration via the ERK signaling pathway

“…IGFBP5, a key member of the IGF axis, has been known to play important roles in diverse biological and pathological processes, including cell proliferation, differentiation, apoptosis and carcinogenesis (Beattie et al, 2006; Karabulut et al, 2016; Sanada et al, 2016). In this study, we demonstrated that dysregulated IGFBP5 expression induced NP cell apoptosis.…”