Correlation between TGF-β1 gene 29 T > C single nucleotide polymorphism and clinicopathological characteristics of osteosarcoma

Abstract

Transforming growth factor (TGF)-β1 is the most abundant growth factor in human bone. Several polymorphisms have been described in the TGF-β1 gene. To explore the correlation between TGF-β1 gene single nucleotide polymorphism and the clinicopathological characteristics of osteosarcoma. TaqMAN PCR technique was used to detect the TGF-β1 gene polymorphism of 124 patients with osteosarcoma from last follow-up and 136 healthy controls. The difference of gender, age, and allele frequency between patient group and control group with χ (2) text were tested. The relationship between single nucleotide polymorphism and the risk of osteosarcoma with logistic regression and different survival rates of different genotypic patients with osteosarcoma through Kaplan-Meier were analyzed. There is no remarkable difference of the three genotypes in TGF-β1 gene 509C > T locus between the patient group and control group (P = 0.26). However, there are significant distributive differences in 29 T > C genotype (P = 0.04), which shows that patients carrying TT genotype have more risk to get osteosarcoma than patients carrying CC genotype (odds ratio (OR) = 2.10, 95 % confidence interval (CI) = 1.08-4.05). The percentage of T allele frequency of patient group, as 60.1 %, is larger than the control group, as 48.9 %. By comparing with patients carrying CC genotype, patients carrying TT genotype have two times risk of metastasis (OR = 2.30, 95 % CI = 1.05-5.06), and most of them are in the period of Enneking IIB (OR = 2.54, 95 % CI = 1.18-5.51). The survival analysis indicates that there is no any significant decrease when there is recurrence in patients carrying TT genotype. The morbidity and metastasis of osteosarcoma are relevant to TGF-β1 gene 29 T > C single nucleotide polymorphism.

Anti-HBV Drugs: Progress, Unmet Needs, and New Hope

“…Thus, it plays a key role in chronic HBV infection-induced liver inflammation and fibrosis. At present, many studies have also examined the molecular structure of TGF-β1 to define the relationship between TGF-β1 and HBV from a perspective of gene polymorphisms [ 84 ]. However, many of the results are inconsistent and unsatisfactory because of ethnic and geographical differences and case selection bias.…”

Associations between inflammatory gene polymorphisms (TNF-α 308G/A, TNF-α 238G/A, TNF-β 252A/G, TGF-β1 29T/C, IL-6 174G/C and IL-10 1082A/G) and susceptibility to osteosarcoma: a meta-analysis and literature review

“…A total of nine studies [ 26 , 29 , 32 , 33 , 36 – 40 ] with 1119 cases and 1358 controls were recruited in this meta-analysis. The process of study selection and the inclusion process are shown in Figure 1 .…”

“…Oliveira et al [ 26 ] and Xie et al [ 37 ] studied the relationship between TNF-β 252A/G and susceptibility to OS. Ma et al [ 38 ], Xu et al [ 39 ] and Wu et al [ 40 ] reported the alleles and genotypes of TGF-β1 29T/C polymorphism. Oliveira et al [ 26 ] reported the alleles and genotypes of IL-6 174G/C and IL-10 1082A/G polymorphism, and these two polymorphisms were also studied by Qi et al [ 32 ] and Cui et al [ 33 ].…”

“…Oliveira et al [ 26 ] reported the alleles and genotypes of IL-6 174G/C and IL-10 1082A/G polymorphism, and these two polymorphisms were also studied by Qi et al [ 32 ] and Cui et al [ 33 ]. Furthermore, seven studies [ 29 , 32 , 33 , 37 – 40 ] were performed in Asian populations, and the other two studies [ 26 , 36 ] were conducted in Caucasian populations. In addition, all these studies had complied with HWE.…”

“…Multiple factors are involved in the etiology of OS, among which genetics and gene polymorphisms have aroused increased attention [ 1 , 4 , 8 ]. Given the important roles of inflammation and gene polymorphisms in cancer etiology and progression, more studies have been performed to explore the associations between inflammatory gene polymorphisms and susceptibility to OS [ 26 , 29 , 32 , 33 , 36 – 40 , 48 , 49 ]. Although several studies including Patio-Garcia et al [ 36 ], Oliveira et al [ 26 ], Zhao et al [ 29 ] and Xie et al [ 37 ] have explored the possible association between TNF-α 308G/A polymorphism and OS risk, there results are inconsistent.…”