Correlation between survivin mRNA expression and lymph node metastasis in gastric cancer

Miyachi, Kazuhito; Sasaki, Kenji; Onodera, Satoshi; Taguchi, T; Nagamachi, Masao; Kaneko, Hiromi; Sunagawa, Masakatsu

doi:10.1007/s10120-003-0255-2

Cited by 53 publications

(40 citation statements)

References 40 publications

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“…54,55 Furthermore, evidence is now accumulating that the expression level of survivin contributes to the clinical outcome of tumors, including prognosis, susceptibility to anti-cancer drugs, and malignant behavior. 56,57 Recent studies indicate the potential clinical relevance of survivin quantified by real-time PCR in ATL. 58 Selectivity of expression and biological importance render survivin an attractive target for treatment, but the 3-dimensional structure of survivin shows no obvious opportunity for drug design.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional activation of survivin through the NF‐κB pathway by human T‐cell leukemia virus type I tax

Kawakami

Tomita

Matsuda

et al. 2005

Intl Journal of Cancer

141

112

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Survivin, a unique member of the inhibitor of apoptosis protein family, is overexpressed in many cancers and considered to play an important role in oncogenesis. We previously reported the survivin expression profile in ATL, a CD4-positive T-cell malignancy caused by HTLV-I. HTLV-I Tax is thought to play an important role in immortalization of T cells. We have shown also that the expression of Tax protected the mouse T-cell line CTLL-2 against apoptosis induced by deprivation of IL-2 and converted its growth from being IL-2 dependent to being IL-2 independent through the NF-kB pathway. In our study, we demonstrate that constitutive expression of survivin was associated with resistance to apoptosis after IL-2 deprivation in Tax-expressing CTLL-2 cells. Transient transfection assays showed that survivin promoter was transactivated by Tax, via the activation of NF-kB. Pharmacological NFkB inhibition resulted in suppression of survivin expression and caused apoptosis of Tax-expressing CTLL-2 cells. Our findings suggest that activated NF-kB signaling contributes directly to malignant progression of ATL by preventing apoptosis, acting through the prosurvival protein survivin. ' 2005 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Transcriptional activation of survivin through the NF‐κB pathway by human T‐cell leukemia virus type I tax

Kawakami

Tomita

Matsuda

et al. 2005

Intl Journal of Cancer

141

112

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“…The expression of apoptotic signal proteins play different roles in the death of gastric cancer cells. The current studies [22,23] have denoted the main mechanism of the interaction of the apoptotic signal proteins to conduct down the apoptotic signal. FasL is polymerized with its corresponding Fas receptors [24,25] .…”

Section: Discussion Discussion Discussion Discussion Discussionmentioning

confidence: 99%

Clinical significance of expression of apoptotic signal proteins in gastric carcinoma tissue

Zhao

Gu²,

Tian³

et al. 2005

WJG

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Abstract Abstract Abstract AIM:To evaluate the expressions of apoptotic signal proteins FADD, TRADD, FasL, Fas, and NFκB in gastric carcinoma tissues and their clinical significance. METHODS:Western blot immune trace method was adopted to detect the expressions of apoptotic signal proteins FADD, TRADD, FasL, Fas, and NFκB in 55 tissue specimens of gastric carcinoma. RESULTS:Five apoptotic signal proteins had different expressions in the gastric carcinoma samples and their expressions were not correlated to age (P = 0.085). Expressions of the FADD, FasL, Fas, and NFκB proteins reduced with increase of the volume of tumor with the exception of increased expression the TRADD protein (64.7-71.1%, P = 0.031). With gradual increase of the malignancy of gastric carcinoma tissues, expressions of the FADD, FasL, and Fas proteins decreased (78.6-28.0%, P = 0.008; 78.6-65.9%, P = 0.071; 100.0-46.3%, P = 0.014), while expressions of the TRADD and NFκB proteins increased (42.9-78.1%, P = 0.063; 78.6-79.1%, P = 0.134). With gradual increase of serum CEA, expression of the FADD protein decreased (62.5-34.0%, P = 0.073), but expressions of the TRADD, FasL, Fas, and NFκB proteins increased (0.0-80.8%, P = 0.005; 62.5-70.2%, P = 0.093; 0.0-70.2%, P = 0.003; 62.5-80.9%, P = 0.075). When compared to the tissues of gastric carcinoma without metastasis, the positive rate of expressions of the FADD and FasL proteins increased, whereas expressions of the TRADD, FADD, and NFκB proteins decreased. There was no significant difference between them (P = 0.095). CONCLUSION:Gastric carcinoma is endurable to Fasrelated apoptosis and apoptotic signal proteins are differently expressed in gastric carcinoma.

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“…In many types of cancers, survivin expression is correlated with reduced apoptosis and unfavorable prognosis (62). Miyachi et al demonstrated that survivin mRNA expression was significantly higher in patients with lymph node metastasis in gastric cancer (63). Lee et al reported that survivin was significantly associated with depth of invasion, tumor stage, poor survival and lymph node metastasis (64).…”

Section: Introductionmentioning

confidence: 99%

CD44v6, MMP-7 and nuclear Cdx2 are significant biomarkers for prediction of lymph node metastasis in primary gastric cancer

Okayama

Kumamoto²,

Saitou³

et al. 2009

Oncol Rep

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Abstract. The aim of this study was to examine the expression of CD44v6, CD54, Cdx2, CXCL5, Cyclin B1, MMP-7, nm23, RCAS1 and Survivin in primary gastric cancer and to investigate whether these molecules were useful in predicting the lymph node status. They were selected as candidates for indicators of lymph node metastasis from various kinds of cancer-associated genes reported previously. In 135 cases of radically resected primary gastric adenocarcinoma, we investigated the association between the expression of these molecules and clinocopathologic factors by immunohistochemistry. The results revealed that the expression of CD44v6 and MMP-7 were significantly associated with lymph node status. By contrast, nuclear Cdx2 expression was found to be inversely correlated with lymph node metastasis. Moreover, multivariate analysis demonstrated that CD44v6, MMP-7 and nuclear Cdx2 were independent predictors for lymph node status. In conclusion, our results suggest that positive expression of both CD44v6 and MMP-7, and negative expression of nuclear Cdx2 may serve as powerful predictors of lymph node metastasis in gastric cancer. Combined evaluation of these markers could be further useful to predict lymph node status clinically.

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Correlation between survivin mRNA expression and lymph node metastasis in gastric cancer

Cited by 53 publications

References 40 publications

Transcriptional activation of survivin through the NF‐κB pathway by human T‐cell leukemia virus type I tax

Transcriptional activation of survivin through the NF‐κB pathway by human T‐cell leukemia virus type I tax

Clinical significance of expression of apoptotic signal proteins in gastric carcinoma tissue

CD44v6, MMP-7 and nuclear Cdx2 are significant biomarkers for prediction of lymph node metastasis in primary gastric cancer

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