Correlation between sperm creatine phosphokinase activity and sperm concentrations in normospermic and oligospermic men

Abstract

Toward the development of biochemical probes for the assessment of sperm function we have measured the activities of sperm creatine-N-phosphotransferase (CPK). There was a highly significant inverse correlation (P less than 0.001 in all comparisons) between sperm CPK activities and sperm concentrations in specimens of normospermic and oligospermic men with greater than 30 million sperm/ml (0.106 +/- 0.01 SEM, N = 90, expressed as CPK U/100 million sperm), 20-30 million sperm/ml (0.333 +/- 0.07 SEM, N = 30) and 10-20 million sperm/ml (0.583 +/- 0.12 SEM, N = 30) when compared with the CPK values of the less than 10 million/ml specimens (2.242 +/- 0.46 SEM, N = 30). Furthermore, the distribution of CPK activities within these four groups showed that 96%, 67%, 43%, and 4% of the samples, respectively, were in the less than 0.250 CPK U/100 million sperm normal range (mean + 2 SD of the greater than 30 million sperm/ml group). However, there was no relationship between sperm CPK activities and the values of sperm motility (P greater than 0.15) or morphology (P = 0.38) in the samples. The migrated sperm fractions (significantly improved in motility and velocity parameters) showed CPK activities lower than the initial semen specimens (P less than 0.01, N = 150). In fact, in some oligospermic men the CPK activities of the migrated sperm fractions were within the range of normospermic samples.(ABSTRACT TRUNCATED AT 250 WORDS)

Nadh-Dependent Oxidoreductase (Diaphorase) Activity and Isozyme Pattern of Sperm in Infertile Men

“…Our findings for diaphorase activity are similar to those reported for some other sperm enzymes participating in the transport of energy, such as creatine kinase [7] and adenylate kinase [3], for which no relationship between the percentage of motile sperms and enzyme activity was found.…”

Creatine Kinase Isoforms in the Seminal Plasma and the Purified Human Sperm

“…CK-BB and CK-MiMi in sperm were reported by Claus et al [2]; CK-BB and CK-MM were reported by Huszar et al [3][4][5]. Our results showed that CK-BB was not observed, but CK-MM and a trace amount of CK-MB were observed.…”

“…For CK, largely four groups of isoforms are present: CK-MM, CK-MB, CK-BB, and CK-MiMi. CK-MM is present in muscle cells, CK-MB in myocardial cells, CK-BB in cerebral cells, and CK-MiMi chiefly in mitochondrial inner membrane [2][3][4][5]. Sperm moves using ATP as an energy source and has a high-energy transportation mechanism, phosphorylcreatine shuttle, similar to other cells in mitochondria of its midpiece to the tail portions [6].…”

Human Sperm Maintain Their Shape Following Decondensation and Denaturation for Fluorescent In Situ Hybridization: Shape Analysis and Objective Morphometry

“…One of these biochemical parameters is the concentration of the enzyme creatine phosphokinase (CK) in sperm, which reflects cytoplasmic retention, the hallmark of diminished maturity [3][4][5]. The CK immunocytochemistry studies of individual spermatozoa have also demonstrated a relationship between cytoplasmic retention and aspects of abnormal sperm morphology, including larger head size, roundness of the head, and increased proportion of amorphous heads.…”