Correlation between SOX2 and Survivin clinical features in patients with salivary adenoid cystic carcinoma

Luo, Yejiao; Liu, Tong; Wei, Fei; Yue, Xiao‐Guang

doi:10.1016/j.jiph.2019.03.015

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…Survivin has been reported to play important roles in regulating cell survival, apoptosis and cell cycle by acting with the apoptosis effectors in various types of malignancies [20]. Recent studies found that survivin was highly expressed in SACC tissues and was correlated with poor prognosis in the patients with SACC [21–23]. In our study, bioinformatics prediction and luciferase reporter assay showed that survivin was targeted and inversely regulated by miR-140-5p in SACC cells.…”

Section: Discussionsupporting

confidence: 59%

MicroRNA-140-5p inhibits salivary adenoid cystic carcinoma progression and metastasis via targeting survivin

2019

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Background Salivary adenoid cystic carcinoma (SACC) is one of the most frequent carcinomas derived from the salivary gland. Growing evidence implied the involvement of microRNAs (miRNAs) in SACC progression and metastasis. This study aimed to determine the regulatory role of miR-140-5p in SACC progression and metastasis and to explore the underlying mechanisms. Materials and methods MiR-140-5p and survivin mRNA expression levels were determined by quantitative real-time PCR; protein levels were evaluated by western blot assay; cell proliferation, growth, invasion, apoptosis and caspase-3 activity were evaluated by respective in vitro functional assays; xenograft nude mice model was used to assess the in vivo tumor growth; a luciferase reporter assay determined the interaction between miR-140-5p and survivin. Results MiR-140-5p overexpression suppressed SACC cell proliferation and invasion, induced cell apoptosis and inhibited in vivo tumor growth of SACC cells. The loss-of-function studies showed that miR-140-5p knockdown enhanced SACC cell proliferation and invasion, inhibited cell apoptosis and led to an accelerated in vivo tumor growth. The bioinformatics prediction and luciferase reporter assay revealed that miR-140-5p directly targeted survivin 3′ untranslated region, and survivin was inversely regulated by miR-140-5p. Knockdown of survivin exerted tumor-suppressive effects on SACC cells, while enforced expression of survivin counteracted the tumor-suppressive actions of miR-140-5p overexpression in SACC cells. Mechanistically, miR-140-5p modulated the protein expression levels of apoptosis- and epithelial-mesenchymal transition-related mediators as well as matrix metallopeptidase-2/-9 via targeting survivin. More importantly, the down-regulation of miR-140-5p and the up-regulation of survivin were detected in the SACC clinical tissues, and miR-140-5 expression was inversely correlated with survivin mRNA expression level in SACC tissues. Conclusion Our data indicated that miR-140-5p suppressed SACC cell proliferation and invasion, induced cell apoptosis via regulating survivin expression. The present study provide evidence that that miR-140-5p could be a promising target for treating SACC, which requires further investigations.

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Section: Discussionsupporting

confidence: 59%

MicroRNA-140-5p inhibits salivary adenoid cystic carcinoma progression and metastasis via targeting survivin

2019

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“…Confocal immunofluorescence using meningioma markers confirmed that the SOX2 signal resides in the nucleus ( Supplementary Figure S1 ). In order to validate SOX2 IHC [ 14 , 15 ], in a subset of specimens (71 samples), mRNA levels of SOX2 were semiquantitatively assessed by RT-qPCR. SOX2 grading by IHC and mRNA levels were significantly correlated (R = 0.4, p = 0.0006, Spearman′s correlation; Supplementary Figure S2A ).…”

Section: Resultsmentioning

confidence: 99%

Dissecting Stemness in Aggressive Intracranial Meningiomas: Prognostic Role of SOX2 Expression

Bonaventura

Martini

Cenci

et al. 2022

IJMS

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Meningiomas are mostly benign tumors that, at times, can behave aggressively, displaying recurrence despite gross-total resection (GTR) and progression to overt malignancy. Such cases represent a clinical challenge, particularly because they are difficult to recognize at first diagnosis. SOX2 (Sex-determining region Y-box2) is a transcription factor with a key role in stem cell maintenance and has been associated with tumorigenesis in a variety of cancers. The purpose of the present work was to dissect the role of SOX2 in predicting the aggressiveness of meningioma. We analyzed progressive/recurrent WHO grade 1–2 meningiomas and WHO grade 3 meningiomas; as controls, non-recurring WHO grade 1 and grade 2 meningioma patients were enrolled. SOX2 expression was evaluated using both immunohistochemistry (IHC) and RT-PCR. The final analysis included 87 patients. IHC was able to reliably assess SOX2 expression, as shown by the good correlation with mRNA levels (Spearman R = 0.0398, p = 0.001, AUC 0.87). SOX2 expression was an intrinsic characteristic of any single tumor and did not change following recurrence or progression. Importantly, SOX2 expression at first surgery was strongly related to meningioma clinical behavior, histological grade and risk of recurrence. Finally, survival data suggest a prognostic role of SOX2 expression in the whole series, both for overall and for recurrence-free survival (p < 0.0001 and p = 0.0001, respectively). Thus, SOX2 assessment could be of great help to clinicians in informing adjuvant treatments during follow-up.

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“…According to Thierauf et al, 33 lack of expression of SOX2 marker mainly occurs in solid and high-grade AdCCs. The study results by Luo et al 34 demonstrated that the expression of SOX2 in AdCC tumoral tissue was more than normal salivary gland tissue, which indicates the tumorigenesis role of SOX2 in this SGC. Moreover, Dai et al 28 revealed a direct relationship between the expression of SOX2 marker and factors such as tumor size and distant metastasis in patients with AdCC.…”

Section: High Expression Low Expressionmentioning

confidence: 84%

Prognostic Significance of Cancer Stem Cell Markers in Patients With Salivary Gland Carcinomas

Sadeghi

Saffar²,

Taheri³

et al. 2022

Applied Immunohistochemistry &Amp; Molecular Morphology

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Objectives: Cancer stem cells (CSCs) are a small group of cells resistant to therapy and play a major role in tumor progression, recurrence, and poor clinical outcomes of patients. This study aimed to evaluate the association of CSC markers with clinicopathologic features and survival in patients with salivary gland carcinomas (SGCs). Materials and Methods:The medical records of 48 patients affected by mucoepidermoid carcinoma (MEC) and 47 patients with adenoid cystic carcinoma (AdCC) were reviewed retrospectively. SOX2, CD133, and CD44 expression was appraised by immunohistochemistry and statistically analyzed to weigh the correlation between these markers and patients' clinicopathologic features and tumor outcomes. Results:In AdCC patients showing poor outcomes, a trend toward a high expression of CD133 and CD44 and low expression of SOX2 was observed, while in MEC patients experiencing the same outcomes, there was a trend toward a high expression of CD44 and low expression of CD133 and SOX2. Only the increase of MEC histopathologic grade was statistically significant with decreased SOX2 expression. Distant metastasis in AdCC patients, tumor grade, lymph node involvement, and local recurrence in MEC patients had significant correlations with patients' survival. Conclusion:Besides the significant association between low SOX2 expression and higher grades of MEC, we found no statistically significant correlation between the studied CSC markers and patients' survival or clinicopathologic features. Therefore, a larger sample size with long-term follow-up is beneficial for thorough investigations toward the main role of CSCs in patients with SGCs.

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Correlation between SOX2 and Survivin clinical features in patients with salivary adenoid cystic carcinoma

Cited by 6 publications

References 24 publications

MicroRNA-140-5p inhibits salivary adenoid cystic carcinoma progression and metastasis via targeting survivin

MicroRNA-140-5p inhibits salivary adenoid cystic carcinoma progression and metastasis via targeting survivin

Dissecting Stemness in Aggressive Intracranial Meningiomas: Prognostic Role of SOX2 Expression

Prognostic Significance of Cancer Stem Cell Markers in Patients With Salivary Gland Carcinomas

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