Correlation between slaughter practices and the distribution of Salmonella and hygiene indicator bacteria on pig carcasses during slaughter

Biasino, W.; Zutter, Lieven De; Mattheus, Wesley; Bertrand, Sophie; Uyttendaele, Mieke; Damme, Inge Van

doi:10.1016/j.fm.2017.10.003

Cited by 40 publications

(36 citation statements)

References 34 publications

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“…in the pig oral cavity can often lead to contamination of the jowls as well as its presence in the animal's intestine leads to contamination in the mesenteric lymph nodes. Several studies have demonstrated that the incision of lymphatic tissues can significantly affect bacterial contamination on the pig carcass [25,26]. In the present study, Salmonella spp.…”

Section: Resultsmentioning

confidence: 49%

Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil

et al. 2019

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Control of Salmonella spp. in food production chains is very important to ensure safe foods and minimize the risks of foodborne disease occurrence. This study aimed to identify the prevalence and main contamination sources of Salmonella spp. in a pig production chain in southern Brazil. Six lots of piglets produced at different farms were tracked until their slaughter, and samples were subjected to Salmonella spp. detection. The obtained isolates were serotyped, subjected to antimicrobial resistance testing, and pulsed field gel electrophoresis (PFGE). Salmonella spp. was detected in 160 (10.2%) samples, and not detected in pig carcasses after final washing or chilling. Among the 210 Salmonella spp. isolates, S. Typhimurium was the most prevalent (n = 101) and resistant to at least one antimicrobial. High resistance rates were detected against tetracycline (83.8%), chloramphenicol (54.3%), and trimethoprim-sulfamethoxazole (33.3%). The isolates that were non-susceptible to three or more classes of antimicrobials (n = 60) were considered multidrug-resistant (MDR), and isolates resistant to up to six of the tested antimicrobials were found. PFGE allowed the identification of genetic diversity and demonstrated that farm environment and feed supply may be sources for the dissemination of Salmonella spp. along the production chain. The results revealed the sources of Salmonella contamination in the pig production chain and highlighted the risks of antimicrobial resistance spread.

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Section: Resultsmentioning

confidence: 49%

Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil

et al. 2019

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“…Apparently, E. coli makes up only a small part of the microbial community in porcine ICLNs but it is still the most active one. The fact that it is metabolically active is concerning, because it is used as an indicator of food hygiene and regularly contaminates pork carcasses (Biasino et al, 2018). Hence, lymph nodes should be considered as a reservoir of pathogenic bacteria, making them a risk hazard for food safety and spoilage.…”

Section: Discussionmentioning

confidence: 99%

Microbiota of the Gut-Lymph Node Axis: Depletion of Mucosa-Associated Segmented Filamentous Bacteria and Enrichment of Methanobrevibacter by Colistin Sulfate and Linco-Spectin in Pigs

et al. 2019

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Microorganisms are translocated from the gut to lymphatic tissues via immune cells, thereby challenging and training the mammalian immune system. Antibiotics alter the gut microbiome and consecutively might also affect the corresponding translocation processes, resulting in an imbalanced state between the intestinal microbiota and the host. Hence, understanding the variant effects of antibiotics on the microbiome of gut-associated tissues is of vital importance for maintaining metabolic homeostasis and animal health. In the present study, we analyzed the microbiome of (i) pig feces, ileum, and ileocecal lymph nodes under the influence of antibiotics (Linco-Spectin and Colistin sulfate) using 16S rRNA gene sequencing for high-resolution community profiling and (ii) ileocecal lymph nodes in more detail with two additional methodological approaches, i.e., cultivation of ileocecal lymph node samples and (iii) metatranscriptome sequencing of a single lymph node sample. Supplementation of medicated feed showed a local effect on feces and ileal mucosa-associated microbiomes. Pigs that received antibiotics harbored significantly reduced amounts of segmented filamentous bacteria (SFB) along the ileal mucosa ( p = 0.048; 199.17-fold change) and increased amounts of Methanobrevibacter , a methanogenic Euryarchaeote in fecal samples ( p = 0.005; 20.17-fold change) compared to the control group. Analysis of the porcine ileocecal lymph node microbiome exposed large differences between the viable and the dead fraction of microorganisms and the microbiome was altered to a lesser extent by antibiotics compared with feces and ileum. The core microbiome of lymph nodes was constituted mainly of Proteobacteria . RNA-sequencing of a single lymph node sample unveiled transcripts responsible for amino acid and carbohydrate metabolism as well as protein turnover, DNA replication and signal transduction. The study presented here is the first comparative study of microbial communities in feces, ileum, and its associated ileocecal lymph nodes. In each analyzed site, we identified specific phylotypes susceptible to antibiotic treatment that can have profound impacts on the host physiological and immunological state, or even on global biogeochemical cycles. Our results indicate that pathogenic bacteria, e.g., enteropathogenic Escherichia coli , could escape antibiotic treatment by translocating to lymph nodes. In general ileocecal lymph nodes harbor a more diverse and active community of microorganisms than previously assumed.

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“…La cantidad de aislamientos de Salmonella observada en las canales del rastro RNC puede estar relacionada con fallas en el proceso de evisceración. Existe evidencia que demuestra que después de la evisceración del ganado porcino, la contaminación por enterobacterias aumenta en la canal y la cantidad de éstas es diferencial en cada una de las partes de la canal (5) , aumentando la contaminación bacteriana en la áreas anteriores y ventrales de la canal cuando hay cortes en las amígdalas durante la remoción de las vísceras, y disminuye cuando las herramientas utilizadas en la remoción de éstas son higienizados posterior a la remoción de las amígdalas (5) . Así mismo, la contaminación observada en el rastro RNC puede ser originada por la contaminación cruzada entre las bacterias presentes en el ganado porcino y bovino, debido a que en el rastro RNC evaluado en este estudio, también se lleva a cabo el sacrificio de ganado bovino (19) .…”

Section: Resultados Y Discusiónunclassified

“…Existe una gran cantidad de reportes que muestran que la frecuencia de bacterias patógenas y resistentes en alimentos es un problema que se presenta en diversos países. La cantidad de cepas patógenas resistentes reportadas es diferente en cada región del mundo, pero dicha cantidad está en relación a la implementación y seguimiento de medidas de control en los procesos de producción de alimentos (4)(5)(6) .…”

Section: Introductionunclassified

Resistencia antimicrobiana de Salmonella spp aisladas de canales de cerdo obtenidas de dos tipos de rastros en Jalisco, México

Vega-Sánchez

Barba-León

González-Aguilar

et al. 2020

Rev. Mex. Cienc. Pecu.

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Salmonella es una de las principales bacterias que originan enfermedades transmitidas por alimentos. El estudio de la resistencia mostrada por Salmonella a diferentes antimicrobianos ha cobrado importancia en los últimos años debido a las complicaciones en el tratamiento de las infecciones causadas por cepas resistentes. Este estudio muestra el perfil de resistencia de cepas de Salmonella aisladas en dos rastros que se diferencian en los procesos de sacrificio y faenado del ganado porcino. Los resultados de este estudio muestran que el rastro que ha implementado y cumple con los procesos sanitarios de obtención de la carne tiene una menor cantidad de aislamientos de Salmonella (1.3 %), que aquel cuyas prácticas de higiene son menos rigurosas (46.8 %) (P<0.05). Los principales serotipos de Salmonella encontrados fueron London (44.7 %), Anatum (15.8 %), Agona, Muenchen y Typhimurium (7.9 %). La resistencia a los aminoglucósidos (100 %), tetraciclinas (73.7 %) y ciprofloxacina (44.7 %) fueron predominantes en los aislamientos evaluados. El 66.6 % de las cepas evaluadas fueron resistentes a 3 ó 4 clases diferentes de antimicrobianos, y se encontró la presencia del gen que codifica para la integrasa 1. Los resultados muestran que Salmonella ha adquirido diferentes elementos genéticos que la vuelven resistente a diferentes clases de antimicrobianos, complicando el tratamiento de una infección causada por este patógeno. Así mismo, sugieren que la implementación y cumplimiento de los procesos sanitarios de obtención de la carne del ganado porcino disminuyen los aislamientos de Salmonella en las canales.

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Correlation between slaughter practices and the distribution of Salmonella and hygiene indicator bacteria on pig carcasses during slaughter

Cited by 40 publications

References 34 publications

Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil

Prevalence, Antimicrobial Resistance, and Diversity of Salmonella along the Pig Production Chain in Southern Brazil

Microbiota of the Gut-Lymph Node Axis: Depletion of Mucosa-Associated Segmented Filamentous Bacteria and Enrichment of Methanobrevibacter by Colistin Sulfate and Linco-Spectin in Pigs

Resistencia antimicrobiana de Salmonella spp aisladas de canales de cerdo obtenidas de dos tipos de rastros en Jalisco, México

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