Correlation between skin tests to Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis in Cuban asthmatics

Almarales, Raúl Lázaro Castro; Morejón, Maytee Mateo; Robalino, R.M. Naranjo; Viltre, Bárbara I. Navarro; Castelló, Mirta Álvarez; Díaz, Mercedes Ronquillo; Gómez, Iris García; Diaz, Yunia Oliva; León, Mayda González; Canosa, José Severino Rodríguez; Labrada, Alexis

doi:10.1157/13084223

Cited by 13 publications

(8 citation statements)

References 9 publications

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“…[1][2][3][4][5] Yet, Blomia tropicalis (Blo t) is an important mite allergen in tropical regions like Singapore, Malaysia, Columbia, and the Taiwan and Hainan provinces of China.…”

Section: Introductionmentioning

confidence: 99%

“…Yet, Blomia tropicalis (Blo t) is an important mite allergen in tropical regions like Singapore, Malaysia, Columbia, and the Taiwan and Hainan provinces of China. [1][2][3][4][5] Blo t coexists with Der p and Der f, and Blo t-sensitized patients are usually co-sensitized to Der p and Der f, with reports of more than 30% of patients sensitized to all three species. 6 Some studies have reported a moderate correlation between Der p and Blo t through analysis of the levels of specific IgE (sIgE).…”

Section: Introductionmentioning

confidence: 99%

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Co‐sensitization and cross‐reactivity of Blomia tropicalis with two Dermatophagoides species in Guangzhou, China

Liu

Zheng

et al. 2019

Clinical Laboratory Analysis

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Background Contradictory results have been reported previously in the analyses of cross‐reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co‐sensitization and the IgE cross‐reactivity among them and attempts to identify whether patients are sensitized to Blo t due to cross‐reaction or true sensitization. Methods Specific IgE (sIgE) in the sera from 1497 allergenic patients was determined by ImmunoCAP. Cross‐reactivity was analyzed and determined by sIgE inhibition with 21 sera samples. Results Around 85.50% of patients were sensitized to Der p, 85.37% of patients were sensitized to Der f, and 71.54% of patients were sensitized to Blo t. Further, 70.14% of patients were co‐sensitized to Blo t, Der p, and Der f, and only seven patients were sensitized solely to Blo t. With increasing sIgE levels for Blo t, the positive rates of severe‐level (class 5‐6) co‐sensitization to Der p or Der f significantly increased. Blo t was moderately associated with Der p and Der f, with correlation coefficients of 0.6998 and 0.6782, respectively. Der p and Der f inhibited IgE binding to Blo t more strongly than Blo t inhibited IgE binding to Der p or Der f in the patient groups CBlo t < CDer p and CBlo t < CDer f. Conclusions This study has established valuable information about the co‐sensitization and cross‐reactivity of Blo t with two Dermatophagoides species (Der p and Der f) and helps to provide adequate diagnosis and treatment of the mite‐allergic patients.

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“…[1][2][3][4][5] Yet, Blomia tropicalis (Blo t) is an important mite allergen in tropical regions like Singapore, Malaysia, Columbia, and the Taiwan and Hainan provinces of China.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Co‐sensitization and cross‐reactivity of Blomia tropicalis with two Dermatophagoides species in Guangzhou, China

Liu

Zheng

et al. 2019

Clinical Laboratory Analysis

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“…Among the mites, Dermatophagoides pteronyssinu s and Blomia tropicalis are the main sources of allergens in sub-tropical and tropical regions of the world [4-6]. High frequencies of positivity to B. tropicalis antigens in skin prick tests have been described in asthma and rhinitis patients, such as 68.1% in Cuba [7], 91.6% in Venezuela [8], 73.3% in Taiwan [9] and 95.0% in São Paulo, Brazil [10]. There is evidence that allergens from B. tropicalis are distinct from, and bear only low to moderate cross-reactivity to allergens from Dermatophagoides sp .…”

Section: Introductionmentioning

confidence: 99%

Respiratory allergy to Blomia tropicalis: Immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model

et al. 2010

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BackgroundThe dust mite Blomia tropicalis is an important source of aeroallergens in tropical areas. Although a mouse model for B. tropicalis extract (BtE)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. The relevance and reproducibility of experimental animal models of allergy depends on the genetic background of the animal, the molecular composition of the allergen and the experimental protocol.ObjectivesThis work had two objectives. The first was to study the anti-B. tropicalis allergic responses in different mouse strains using a short-term model of respiratory allergy to BtE. This study included the comparison of the allergic responses elicited by BtE with those elicited by ovalbumin in mice of the strain that responded better to BtE sensitization. The second objective was to investigate whether the best responder mouse strain could be used in an experimental model of allergy employing relatively low BtE doses.MethodsGroups of mice of four different syngeneic strains were sensitized subcutaneously with 100 μg of BtE on days 0 and 7 and challenged four times intranasally, at days 8, 10, 12, and 14, with 10 μg of BtE. A/J mice, that were the best responders to BtE sensitization, were used to compare the B. tropicalis-specific asthma experimental model with the conventional experimental model of ovalbumin (OVA)-specific asthma. A/J mice were also sensitized with a lower dose of BtE.ResultsMice of all strains had lung inflammatory-cell infiltration and increased levels of anti-BtE IgE antibodies, but these responses were significantly more intense in A/J mice than in CBA/J, BALB/c or C57BL/6J mice. Immunization of A/J mice with BtE induced a more intense airway eosinophil influx, higher levels of total IgE, similar airway hyperreactivity to methacholine but less intense mucous production, and lower levels of specific IgE, IgG1 and IgG2 antibodies than sensitization with OVA. Finally, immunization with a relatively low BtE dose (10 μg per subcutaneous injection per mouse) was able to sensitize A/J mice, which were the best responders to high-dose BtE immunization, for the development of allergy-associated immune and lung inflammatory responses.ConclusionsThe described short-term model of BtE-induced allergic lung disease is reproducible in different syngeneic mouse strains, and mice of the A/J strain was the most responsive to it. In addition, it was shown that OVA and BtE induce quantitatively different immune responses in A/J mice and that the experimental model can be set up with low amounts of BtE.

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“…However, less attention has been paid to another major mite allergen, Blomia tropicalis, which is prevalent in tropical and subtropical regions of the world (7,8), where .2 billion people live. High frequencies of sensitivity to B. tropicalis have been described in asthma and rhinitis patients in those regions, and .20 allergens were identified through their IgE-binding activity (9)(10)(11)(12). Most of these allergens only have 30-40% sequence identity with their D. pteronyssinus counterparts and possess low IgE cross-reactivity (13).…”

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confidence: 99%

GM-CSF–Licensed CD11b+ Lung Dendritic Cells Orchestrate Th2 Immunity to Blomia tropicalis

Zhou

Schlitzer

et al. 2014

The Journal of Immunology

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The Blomia tropicalis dust mite is prevalent in tropical and subtropical regions of the world. Although it is a leading cause of asthma, little is known how it induces allergy. Using a novel murine asthma model induced by intranasal exposure to B. tropicalis, we observed that a single intranasal sensitization to B. tropicalis extract induces strong Th2 priming in the lung draining lymph node. Resident CD11b+ dendritic cells (DCs) preferentially transport Ag from the lung to the draining lymph node and are crucial for the initiation of Th2 CD4+ T cell responses. As a consequence, mice selectively deficient in CD11b+ DCs exhibited attenuated Th2 responses and more importantly did not develop any allergic inflammation. Conversely, mice deficient in CD103+ DCs and CCR2-dependent monocyte-derived DCs exhibited similar allergic inflammation compared with their wild-type counterparts. We also show that CD11b+ DCs constitutively express higher levels of GM-CSF receptor compared with CD103+ DCs and are thus selectively licensed by lung epithelial-derived GM-CSF to induce Th2 immunity. Taken together, our study identifies GM-CSF–licensed CD11b+ lung DCs as a key component for induction of Th2 responses and represents a potential target for therapeutic intervention in allergy.

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Correlation between skin tests to Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis in Cuban asthmatics

Cited by 13 publications

References 9 publications

Co‐sensitization and cross‐reactivity of Blomia tropicalis with two Dermatophagoides species in Guangzhou, China

Co‐sensitization and cross‐reactivity of Blomia tropicalis with two Dermatophagoides species in Guangzhou, China

Respiratory allergy to Blomia tropicalis: Immune response in four syngeneic mouse strains and assessment of a low allergen-dose, short-term experimental model

GM-CSF–Licensed CD11b+ Lung Dendritic Cells Orchestrate Th2 Immunity to Blomia tropicalis

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