Correlation between serum levels of soluble tumor necrosis factor receptor and disease activity in systemic lupus erythematosus

Aderka, Dan; Wysenbeek, A J; Engelmann, Hartmut; Cope, Andrew P.; Brennan, Fionula M.; Molad, Yair; Hornik, V; Levo, Y; Maini, R. N.; Feldmann, M

doi:10.1002/art.1780360812

Cited by 167 publications

(104 citation statements)

References 39 publications

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“…17 High levels of proinflammatory cytokines have been reported in inflammatory and infectious diseases and can be correlated with disease severity. 32,33 In this study, we did not find any other clinical illness that could explain the high levels of proinflammatory cytokines. On the other hand, our study population was small and, therefore, confounding factors could not be taken into account.…”

Section: Discussioncontrasting

confidence: 57%

Serum cytokine levels in patients with chronic low back pain due to herniated disc: analytical cross-sectional study

et al. 2010

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CONTEXT AND OBJECTIVE: The role of immune response and proinflammatory cytokines in the pathogenesis of chronic pain has been of growing interest. In order to evaluate whether there is any association between disc herniation and elevated cytokine levels, we measured cytokine levels in patients with chronic low back pain and in healthy subjects. DESIGN AND SETTING:Analytical cross-sectional study at the Pain Clinic of Universidade Federal da Bahia (UFBA). METHODS:Cytokine levels were measured using the enzyme-linked immunosorbent assay (ELISA) technique on 23 patients with low back pain (G1) and on 10 healthy subjects (G2). RESULTS:The levels of tumor necrosis factor-alpha [TNF-alpha] (G1 = 5.6 ± 2.3 pg/ml; G2 = 1.6 ± 0.5 pg/ml; P = 0.01) and interleukin-6[IL-6] (G1 = 4.1 ± 3.0 pg/ml; G2 = 0.9 ± 0.4 pg/ml; P = 0.01) were higher in G1. There were no statistically significant differences in relation to interleukin-1 [IL-1] (G1 = 0.5 ± 0.3 pg/ml; G2 = 0.5 ± 0.1 pg/ml; P = 1) or soluble tumor necrosis factor receptor [sTNF-R] (G1 = 572 pg/ml ± 36; G2 = 581 ± 50 pg/ml; P = 0.87). CONCLUSION:The patients with chronic low back pain due to disc herniation presented higher levels of TNF-alpha and IL-6, but not of IL-1 or sTNF-R. RESUMO CONTEXTO E OBJETIVO:A função da resposta imunológica e das citocinas pró-inflamatórias na patogênese da dor crônica tem tido interesse crescente. Para avaliar se há correlação entre hérnia de disco e aumento de citocinas, foi medida a concentração de citocinas em pacientes com lombalgia crônica e em indivíduos sadios. TIPO DE ESTUDO E LOCAL: Estudo transversal analítico realizado na Clínica de Dor da Universidade Federal da Bahia (UFBA).MÉTODO: As concentrações de citocinas foram medidas pela técnica de ELISA (enzyme linked immunosorbent assay) em 23 pacientes com lombalgia (G1) e 10 sadios (G2). RESULTADOS:As concentrações de fator-alfa de necrose tumoral [TNF-alpha] (G1 = 5.6 ± 2.3 pg/ml; G2 = 1.6 ± 0.5 pg/ml; P = 0,01) e interleucina-6 [IL-6] (G1 = 4.1 ± 3.0 pg/ml; G2 = 0.9 ± 0.4 pg/ml; P = 0,01) foram maiores no G1. Não houve diferença estatisticamente significante para interleucina-1 [IL-1] (G1 = 0.5 ± 0.3 pg/ml; G2 = 0.5 ± 0.1 pg/ml; P = 1) e receptor solúvel do factor de necrose tumoral [sTNF-R] (G1 = 572 pg/ml ± 36; G2 = 581 ± 50 pg/ml; P = 0,87).CONCLUSÃO: Os pacientes com lombalgia crônica por hérnia de disco apresentam concentrações maiores de TNF-alpha e IL-6, mas não de IL-1 ou sTNF-R.

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Section: Discussioncontrasting

confidence: 57%

Serum cytokine levels in patients with chronic low back pain due to herniated disc: analytical cross-sectional study

et al. 2010

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“…The association between decreased HDL cholesterol levels and active disease was not surprising, since decreased HDL levels have been reported to be part of the acute-phase response (44), and it has been suggested that high levels of tumor necrosis factor ␣ present in patients with SLE were associated with a lowering of HDL cholesterol levels (28,(45)(46)(47). In years 1-3, only 1 patient had a HDL cholesterol level below that of the age-and sex-matched controls, and the mean values were not significantly different from those in the controls.…”

Section: Sarkissian Et Almentioning

confidence: 99%

The complex nature of the interaction between disease activity and therapy on the lipid profile in patients with pediatric systemic lupus erythematosus

Sarkissian¹,

Beyenne²,

Feldman³

et al. 2006

Arthritis & Rheumatism

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Objective. To determine the prevalence of lipid abnormalities at different times and to determine the influence of both the disease and corticosteroid therapy on lipid abnormalities in pediatric patients with systemic lupus erythematosus (SLE).Methods. Lipid measurements were obtained in an inception cohort of 139 pediatric patients with SLE (114 females). Fasting levels of total cholesterol, triglycerides, low-density lipoprotein (LDL) cholesterol, and highdensity lipoprotein (HDL) cholesterol in the SLE patients were compared with those in age-and sex-matched control subjects. Disease activity levels and medication dosages were obtained at the time of lipid measurements.Results. At the time of diagnosis, the mean levels of total cholesterol, LDL cholesterol, and triglycerides were highest, whereas the mean levels of HDL cholesterol were lowest. The percentage of patients with abnormal triglyceride values was highest at diagnosis, decreased at year 1, and then remained relatively constant thereafter. The mean total cholesterol and LDL cholesterol levels decreased at year 1 as compared with the time of diagnosis and then remained relatively constant. The lowest mean HDL cholesterol levels were found at the time of diagnosis, and these values rose with time. Comparison of lipid levels at different prednisone dosages and disease activity levels revealed that changes in triglyceride levels were mainly associated with changes in disease activity, changes in both total cholesterol and LDL cholesterol levels were associated with changes in the prednisone dosage and not disease activity, and low levels of HDL cholesterol were associated with active SLE, whereas the prednisone dosage was associated with increased levels of HDL cholesterol.Conclusion. Factors intrinsic to SLE appear to alter lipid levels. Control of SLE may be the most important factor in improving abnormal lipid profiles, and paradoxically, prednisone therapy may improve abnormal lipid levels.

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“…sTNF-R exists in two different forms, type I (p55) and type II (p75), isolated from either urine or sera, from both healthy donors and cancer patients (9). These proteins function as inhibitors of TNF␣ activity (10).…”

mentioning

confidence: 99%

The epitopes for natural polyreactive antibodies are rich in proline

Tchernychev

Cabilly

Wilchek

1997

Proc. Natl. Acad. Sci. U.S.A.

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ABSTRACT''Natural'' polyreactive antibodies, which bind in a nonspecific manner to a range of biological molecules both of self-and nonself-origin, are normal constituents of serum and are a significant part of the immune repertoire in many species, including humans. Autoantibodies to sTNF-R (the 55-kDa extracellular domain of the human receptor to tumor necrosis factor ␣) were affinity purified from normal human sera using immobilized sTNF-R. The isolated antisTNF-R IgG bound both native and denatured forms of the receptor with low affinity. These antibodies also bound to different proteins and therefore are considered to be polyreactive. We used the anti-sTNF-R antibodies and purified polyreactive antibodies to mannose-specific lectin from garlic (Allium sativum) for screening a peptide library displayed on filamentous M13 phage. After the biopanning procedure, we failed to find epitopes with a consensus sequence; however, we found that proline is the most frequent amino acid in the selected phagotopes. Proline is commonly present at solventexposed sites in proteins, such as loops, turns, N-terminal first turn of helix, and random coils. Thus, structures containing proline can serve as conformation-dependent common ''public'' epitopes for polyreactive natural antibodies. Our findings may be important for understanding polyreactivity in general and for the significance of polyreactive natural antibodies in immunological homeostasis.

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Correlation between serum levels of soluble tumor necrosis factor receptor and disease activity in systemic lupus erythematosus

Cited by 167 publications

References 39 publications

Serum cytokine levels in patients with chronic low back pain due to herniated disc: analytical cross-sectional study

Serum cytokine levels in patients with chronic low back pain due to herniated disc: analytical cross-sectional study

The complex nature of the interaction between disease activity and therapy on the lipid profile in patients with pediatric systemic lupus erythematosus

The epitopes for natural polyreactive antibodies are rich in proline

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