Correlation Between Renal Activity Index and C3 Complement Expression in Mouse Lupus Nephritis Model

Abstract

Background: Lupus nephritis is a disease which currently has no definitive therapy. For lupus nephritis, histological examination remains the best option to assess the degree of disease activity, which is essential for therapy evaluation and monitoring. At present, there is no single laboratory that can be used to assess disease activity. Therefore, this study aims to determine the correlation between renal activity index and C3 complement expression in mouse lupus nephritis model. Research methods: The study was conducted on male mice. Lupus was induced using intraperitoneal pristane injection at 0.5 cc, then left for four months. At the end of the 4th month, the mice were sacrificed, and histological examination of mice's kidneys and renal C3 complement expressions were evaluated. Statistical tests were done using t-test, Mann-Whitney test, and Spearman correlation test, with a significance level of P <0.05. Results: activity index on histological examination of mouse kidney in such aspects as glomerular proliferation, karyorrhexis, and fibrinoid necrosis, cellular crescents, hyaline deposits, and interstitial inflammation were associated with C3 complement expression in kidney tissue (r = 0.680; p = 0.001) Conclusion: The study demonstrateds that the activity index in kidney histological examination correlates with C3 complement expressions in the lupus nephritis model.

Pengaruh Ekstrak Daun Moringa Oleifera terhadap Mean Platelet Volume dan Neutrophil-To-Lymphocyte Ratio pada Pasien Autoimun

“…Selain itu, Moringa oliefera akan memiliki efek antiinflamasi dengan menghambat nfĶβ (Berkovich et al, 2013). Penghambatan nfĶβ akan menyebabkan penurunan sitokin proinflamasi IL 6, IL 1, dan TNF α sehingga inflamasi jaringan menurun (Prabowo, Nurudhin, & Novia, 2021). Selain itu, Moringa Oleifera telah digunakan untuk mengurangi kerusakan karena rheumatoid arthritis.…”

“…Moringa oliefera memiliki efek anti inflamasi dengan menghambat nfĶβ. Hambatan NfĶβ menyebabkan penurunan sitokin proinflamasi IL 6, IL 1, dan TNF α sehingga inflamasi jaringan berkurang (Prabowo et al, 2021). (Fathir, M. Rifai, 2015) Hasil penelitian menunjukkan bahwa dosis ekstrak daun kelor memiliki dua pengaruh terhadap sel T, limfosit T CD4 / sel T helper, yaitu dosis rendah memiliki efek imunostimulan, dan dosis tinggi menyebabkan imunosupresi.…”

The Effect of Snail Mucus (Achatina Fulica) Toward The Activity and Chronicity Indices of Renal Histology in Pristane-Induced Lupus Nephritis Mice Model

“…Systemic lupus erythematosus (SLE) is a common autoimmune rheumatic disease with a complex mechanism and pathogenesis [1][2][3][4][5] . SLE can affect the kidney, resulting in nephritis due to the presence of the immune complex in the glomerular basement membrane (GBM) 3,6,7 . Nephritis lupus is the most common and severe manifestation found in SLE, with the prevalence as high as 30-82% in Asia Pacific.…”

“…Nephritis lupus is the most common and severe manifestation found in SLE, with the prevalence as high as 30-82% in Asia Pacific. Kidney involvement predicts morbidity and mortality in SLE (3,8,9) . The gold standard in diagnosing lupus nephritis is kidney biopsy.…”

“…The gold standard in diagnosing lupus nephritis is kidney biopsy. From the histological pattern, activity and chronicity indices can be measured to predict the prognosis and response to treatment 3,10 .…”