Correlation between Proteinuria Level and Renal Morphology with Special Reference to Electron Microscopy in Kidney Donors

Nacar, Ahmet; Karabay, Gülten; Unlükal, Nejat; Yazici, Canan; Özdemir, Handan

doi:10.1080/08860220802064713

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“…Previous studies have related renal pathology to prionuria [24], [25], a finding which corresponds to our identification of mild to moderate nephritis in those deer providing samples for the current study. It is plausible that renal pathology contributed to prionuria in each of these animals; as samples were pooled, however, we cannot identify specific animals in which it may have been occurring, nor can we accurately estimate the relative level of prionuria occurring in each donor as ultrastructural studies were not performed [26]. While we have not yet identified pathologic prions in renal source tissues [Unpublished data], protease-resistant PrP CWD has been identified by immunostaining in renal tissue of prion-infected deer [27], sheep [28], hamsters and most intriguingly humans [29], foreshadowing the potential for prionuria in other transmissible spongiform encephalopathies.…”

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confidence: 99%

Detection of CWD Prions in Urine and Saliva of Deer by Transgenic Mouse Bioassay

et al. 2009

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Chronic wasting disease (CWD) is a prion disease affecting captive and free-ranging cervids (e.g. deer, elk, and moose). The mechanisms of CWD transmission are poorly understood, though bodily fluids are thought to play an important role. Here we report the presence of infectious prions in the urine and saliva of deer with chronic wasting disease (CWD). Prion infectivity was detected by bioassay of concentrated, dialyzed urine and saliva in transgenic mice expressing the cervid PrP gene (Tg[CerPrP] mice). In addition, PrPCWD was detected in pooled and concentrated urine by protein misfolding cyclic amplification (PMCA). The concentration of abnormal prion protein in bodily fluids was very low, as indicated by: undetectable PrPCWD levels by traditional assays (western blot, ELISA) and prolonged incubation periods and incomplete TSE attack rates in inoculated Tg(CerPrP) mice (373±3days in 2 of 9 urine-inoculated mice and 342±109 days in 8 of 9 saliva-inoculated mice). These findings help extend our understanding of CWD prion shedding and transmission and portend the detection of infectious prions in body fluids in other prion infections.

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