Correlation Between Percentage of Immuncompetent CD4 +  and CD4 + CD25 +  Cells and Compatibility in the HLA System and Selected Parameters Assessing Transplanted Kidney Function

Sieńko, Jerzy; Kotowski, Maciej; Dziewanowski, K.; Wojciuk, Bartosz; Paczkowska, Edyta; Kędzierska, Karolina; Tejchman, Karol; Stasiuk, Robert; Zair, Labib; Ostrowski, Marek

doi:10.1016/j.transproceed.2018.04.053

Cited by 1 publication

(1 citation statement)

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“…Analysis of immunocompetent and stem cells was performed using flow cytometry according to the methodology described in the author’s previously published articles [ 62 , 63 , 64 ].…”

Section: Methodsmentioning

confidence: 99%

Microchimerism as Post-Transplant Marker of a Chronic Rejection Process

Sieńko,

Kotowski,

Czarnecka

et al. 2023

IJMS

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The risk of losing a transplanted organ is high, and non-invasive markers to warn of this phenomenon are still being sought. We investigated the impact of post-transplant microchimerism on the function of the transplanted kidney. The study included 100 kidney transplant recipients, mostly women. All transplanted organs were from opposite-sex deceased donors. Microchimerism was assessed using multiplex PCR. Male DNA was detected in all urine samples from female recipients and in 13/56 blood samples from female kidney recipients. Female DNA was found in 31/44 urine samples from male recipients, but in none of the blood samples. Microchimerism in the urine of female recipients correlated positively with blood urea (Rs = 0.45; p = 5.84 × 10−4) and K+ ions (Rs = 0.29; p = 0.03), while microchimerism in the blood of female recipients also correlated positively with blood urea (Rs = 0. 28; p = 0.04), cystatin C (Rs = 0.31; p = 0.02) and the number of incompatible HLA alleles (Rs = 0.42; p = 0.01). A history of DGF was associated with higher urinary donor DNA concentrations in female recipients.: Post-transplant microchimerism may serve as a potential marker of chronic kidney rejection.

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“…Analysis of immunocompetent and stem cells was performed using flow cytometry according to the methodology described in the author’s previously published articles [ 62 , 63 , 64 ].…”

Section: Methodsmentioning

confidence: 99%