Correlation between neutralizing antibody titre and protection from tenosynovitis in avian reovirus infections

Takase, Kozo; Fujikawa, Hideo; Yamada, Shinji

doi:10.1080/03079459608419183

Cited by 9 publications

(2 citation statements)

References 15 publications

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“…The importance of neutralizing antibodies and their level in protection against tenosynovitis caused by ARV has been previously demonstrated (Takase et al, 1996). The protective ability of antibodies was found to be type specific, with marginal protection against strains of heterologous ARV serotypes (Meanger et al, 1997).…”

Section: Discussionmentioning

confidence: 83%

Genetic and antigenic characterization of sigma C protein from avian reovirus

Goldenberg¹,

Pasmanik‐Chor²,

Pirak³

et al. 2010

Avian Pathology

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Avian reovirus (ARV) causes viral arthritis, tenosynovitis, liver infection and immunosuppression in birds. Live-attenuated and inactivated vaccines for ARV are available, but do not efficiently protect against recent variants. Sigma C, which mediates virus attachment to target cells, is the most variable protein in ARV. Antibodies to this protein neutralize viral infection. The purpose of the present study was to characterize sigma C in isolates of ARV from infected birds, as compared with the vaccine strain. Amino acids 27 to 293 of sigma C from 28 Israeli isolates were compared, classified and analysed using bioinformatics tools. Large variations were found among the isolates, and the vaccine strain was shown to differ from most of the studied strains, which could explain the failure of commonly used vaccinations in protecting birds against ARV infection. Based on sigma C protein sequences from all over the world, ARV can be divided into four groups. Isolates from all groups were found in the field simultaneously, possibly explaining the insufficient protection achieved by the vaccine strain, which is represented in one of the groups. The results point out the need and the difficulty in producing a wide-ranging vaccine. Several conserved regions among all reported ARV sigma C proteins were identified. These peptides were further studied for structural and functional properties, and for antigenic characterization. The results of this study shed light on peptide selection for a broad and efficient vaccine.

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Section: Discussionmentioning

confidence: 83%

Genetic and antigenic characterization of sigma C protein from avian reovirus

Goldenberg¹,

Pasmanik‐Chor²,

Pirak³

et al. 2010

Avian Pathology

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“…It has been shown that there is an age-related resistance to tenosynovitis caused by avian reovirus infection (Jones & Georgiou, 1984), but that it is therefore important to protect chickens at an early age. Maternal antibodies in progeny from vaccinated breeder flocks offer this protection (Van der Heide et al, 1976;Eidson et al, 1979;Wood & Thornton, 1981;Jones & Nwajei, 1985, Takase et al, 1996aMeanger et al, 1997). Another way to achieve early protection is by vaccination with attenuated reovirus vaccines at 1 day of age.…”

Section: Discussionmentioning

confidence: 99%

A reovirus challenge model applicable in commercial broilers after live vaccination

Loon¹,

Suurland²,

Marel³

2002

Avian Pathology

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The efficacy of live reovirus vaccines may be determined by challenge via the foot pad route 3 to 4 weeks after vaccination. Swelling and discoloration in the foot pad and shank are scored for a period of 14 days. The major disadvantages of this challenge model are the subjective judgement of gross foot pad and/or shank lesions, that it is very difficult to induce lesions in broilers, and that it causes animal suffering. Other reovirus challenge models are based on reisolation of the virus from different tissues or on scoring microscopic lesions in the tendons. Some disadvantages of these models are that they either cannot be used after vaccination with live reovirus because they cannot discriminate between vaccine and challenge virus or that the microscopic lesions scored need not necessarily be related to the challenge virus but may have been induced by other factors. Therefore, we have attempted to develop a reovirus challenge model that was an improvement on the existing ones, using isolation of reovirus from different organs followed by specific detection of the challenge virus with a monoclonal antibody that can discriminate between challenge and vaccine virus. The reovirus challenge model was examined in specific pathogen free (SPF) White Leghorn chickens and commercial broilers. In vivo studies were conducted to examine the efficacy of an attenuated reovirus vaccine in SPF White Leghorn chickens and commercial broilers with maternal immunity against reovirus. No challenge virus could be detected in any of the organs of the vaccinated chickens 3 and 10 days after challenge. In contrast, challenge virus could be isolated from the unvaccinated control group. At an increased challenge dose all unvaccinated challenge control birds were positive, while the vaccinated chickens were protected. It was shown that 1-day-old vaccination in the presence of maternal immunity was effective. It seemed that protection induced in broilers by the attenuated reovirus vaccine may not have been entirely humoral because in protected birds no antibodies against reovirus were detected by enzyme-linked immunosorbent at the time of challenge. Protection in these birds might therefore have been induced by cellular immunity.

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Avian Reovirus Infections

Pitcovski

Goyal²

2019

Diseases of Poultry

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Correlation between neutralizing antibody titre and protection from tenosynovitis in avian reovirus infections

Cited by 9 publications

References 15 publications

Genetic and antigenic characterization of sigma C protein from avian reovirus

Genetic and antigenic characterization of sigma C protein from avian reovirus

A reovirus challenge model applicable in commercial broilers after live vaccination

Avian Reovirus Infections

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