Correlation between neutral alpha-glucosidase activity and sperm DNA fragmentation

Watanabe, M.; Roussev, Roumen G.; Ahlering, Peter; Sauer, R; Coulam, C.B.; Jeyendran, Rajasingam S.

doi:10.1111/j.1439-0272.2009.00950.x

Cited by 10 publications

(5 citation statements)

References 16 publications

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“…Reducing the concentration of NAG in human semen has been shown to be associated with a significant increase in DNA fragmentation [37], results that are consistent with those of the present study, where we show a correlation between NAG levels and SCD with a value of r = −0.70. One of the most important antioxidant enzymes produced by the human epididymis (body and tail) is glutathione peroxidase [27]; it is possible that damage to the epididymis, reflected by a decrease in NAG levels, could be associated with a decrease in production of glutathione peroxidase; increased production of ROS and DNA fragmentation then occurs.…”

Section: Discussionsupporting

confidence: 93%

Varicocele decreases epididymal neutral α‐glucosidase and is associated with alteration of nuclear DNA and plasma membrane in spermatozoa

Vivas-Acevedo¹,

Lozano-Hernández²,

Camejo

2014

BJU International

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Objective• To assess the relationship between a marker of epididymal function and both the fragmentation of the sperm nucleus and the integrity and maturity of the sperm membrane in patients with or without varicocele. Patients and Methods• Semen samples were obtained from men with varicocele grades II and III (n = 60) and from a control group with zoospermia defined as normal (n = 30). • Samples were evaluated by a spermiogram, a hypoosmotic swelling test (HOST), neutral α-glucosidase (NAG) enzyme activity, sperm hyaluronan-binding assay (HBA) and DNA fragmentation using a sperm chromatin dispersion (SCD) test. Results• Seminal plasma NAG levels, percentage of sperm bound to hyaluronic acid, HOST-positive cells and sperm quality were significantly lower in the varicocele compared with the control group.• Higher levels of sperm DNA fragmentation, as measured by SCD, were also observed in the varicocele group compared with the control group.• Seminal NAG activity levels showed a strong negative correlation with DNA fragmentation and a significant positive correlation with the HBA test and the HOST. Conclusions• Varicocele causes a reduction in NAG activity by the epididymis that is associated with damage to both the membrane and sperm nucleus and a reduction in the seminal parameters.• NAG levels were correlated with the quality of the sperm membrane and nucleus.• Data suggest that a reduction of fertilization capacity in men during varicocele can result from damage to both the testis and the epididymis.

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Section: Discussionsupporting

confidence: 93%

Varicocele decreases epididymal neutral α‐glucosidase and is associated with alteration of nuclear DNA and plasma membrane in spermatozoa

Vivas-Acevedo¹,

Lozano-Hernández²,

Camejo

2014

BJU International

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“…Our findings are consistent with the hypothesis that NAG activity in semen might be detectable earlier than spermatozoa after surgery. Our data also show that NAG activity has a moderate positive relationship with TMSC, possibly because NAG activity may reflect patency between the epididymis and vas deferens, epididymal function (Azenabor et al, ) and infection of the reproductive tract (Watanabe et al, ). Elevated NAG activity after MVE may indicate good patency, normal epididymal function and the absence of a serious reproductive tract infection.…”

Section: Discussionsupporting

confidence: 60%

Seminal plasma neutral alpha‐glucosidase activity as an early predictor of patency and natural pregnancy after microsurgical vasoepididymostomy

Zhang

et al. 2019

Andrologia

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Microsurgical vasoepididymostomy (MVE) is recommended as a first-line option for treatment of epididymal obstructive azoospermia (EOA). However, early indicators for predicting patency and natural pregnancy are unclear. Our aim was to explore the early predictive value of seminal plasma neutral alpha-glucosidase (NAG) activity for patency and natural pregnancy after MVE. Eighty-four patients with EOA who underwent MVE were enrolled in this study. The post-operative patency and natural pregnancy rates were 60.71% and 33.33% respectively. The presence of motile epididymal spermatozoa at the anastomosis site and NAG activity measured at the first month after MVE were early and independent predictors of patency and natural pregnancy. The areas under the receiver operating characteristic curves (AUCs) of NAG activity for prediction of patency and natural pregnancy were 0.78 (95% confidence interval [CI]: 0.68-0.88) and 0.82 (95% CI: 0.73-0.92). The best cut-off values of NAG activity for predicting patency and pregnancy were 15.9 and 17.0 m IU/ejaculate respectively. In conclusion， NAG activity measured at the first month after MVE is an early and independent predictor of patency and natural pregnancy. K E Y W O R D Sneutral alpha-glucosidase, patency, pregnancy, vasoepididymostomy

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“…In rats, γ-glutamyl transpeptidase present in the lumen of the proximal region of the epididymis regulates the levels of glutathione and taurine, which protect spermatozoa against ROS (Hinton, Palladino, Mattmueller, Bard, & Good, 1991). Thus, compromised epididymal integrity is associated with a reduced antioxidant activity and increase in sperm DNA fragmentation (Watanabe et al, 2009).…”

Section: Oxidative Stress and Dna Fragmentationmentioning

confidence: 99%

Epididymal contribution to male infertility: An overlooked problem

Elbashir

Magdi²,

Rashed

et al. 2020

Andrologia

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The diagnosis and treatment of male infertility, excluding assisted conception, are limited because of, but not limited to, poor understanding of sperm post-testicular development and storage. Many may think that sperm dysfunction is only self-contained in the sperm cell itself as a result of defective spermatogenesis. However, it can also be a consequence of inadequate epididymal maturation following disorders of the epididymis. Improper epididymal functions can disturb semen parameters and sperm DNA integrity, result in high leucocyte concentrations and high numbers of immature germ cells and debris or even cause idiopathic infertility. To date, the data are limited regarding critical markers of sperm maturation and studies that can identify such markers for diagnosis and managing epididymal dysfunction are scarce. Therefore, this article aims to draw attention to recognise a disturbed epididymal environment as a potential cause of male infertility.

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Correlation between neutral alpha-glucosidase activity and sperm DNA fragmentation

Cited by 10 publications

References 16 publications

Varicocele decreases epididymal neutral α‐glucosidase and is associated with alteration of nuclear DNA and plasma membrane in spermatozoa

Varicocele decreases epididymal neutral α‐glucosidase and is associated with alteration of nuclear DNA and plasma membrane in spermatozoa

Seminal plasma neutral alpha‐glucosidase activity as an early predictor of patency and natural pregnancy after microsurgical vasoepididymostomy

Epididymal contribution to male infertility: An overlooked problem

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