Correlation between neuronal antibodies and limbic encephalitis in Chinese Han subjects

Abstract

ABSTRACT.A variety of anti-neuronal cell membrane antibodies such as voltage-gated potassium channel antibody, N-methyl-Daspartate-2B-antibody, and glutamic acid decarboxylase antibody, are correlated with limbic encephalitis (LE). In this study on patients with LE, the clinical manifestations, psychology Wechsler Adult Intelligence Scale, cerebrospinal fluid, electrophysiology, magnetic resonance imaging, and anti-immune therapy were studied and immunological determination was conducted; it was found that patients of Chinese Han nationality showed 2 types of clinical manifestations: simple and complex. Lesions could also be divided into focal and scalable lesions, and the clinical manifestations and lesions scopes were associated with various antibodies and antibody types. The prognosis may improve if early diagnosis is conducted and early anti-immune therapy is implemented in LE patients.

Autoantibodies detection in anti-N-methyl-D-aspartate receptor encephalitis

“…A positive group showed specific binding with fluorescent markers after incubation with anti-human immunoglobulin G (IgG) with fluorescent labeling. The steps and results of IHC analysis were similar to those of immunofluorescence ( 24 ). The IHC analysis could detect the presence of most antibodies, but lacks specificity.…”

“…If anti-NMDAR antibodies are detected in CSF, the diagnosis can be confirmed owing to its high specificity (16). Four different techniques are used to detect antibodies in anti-NMDAR encephalitis: tissue-based assay (TBA) on the brain tissue of rodents using indirect immunohistochemical (IHC) analysis or-indirect immunofluorescence, culture of dissociated hippocampal neurons from rats, and cell-based assay (CBA) with HEK293 cells (22,24).…”