Correlation Between Mucosal IL-6 mRNA Expression Level and Virulence Factors of Helicobacter pylori in Iranian Adult Patients With Chronic Gastritis

Abstract

Background:Helicobacter pylori infection is associated with gastritis and marked infiltration of the gastric mucosa by several cytokines secreting inflammatory cells that contribute to sustained local inflammation. In this study, we sought to examine IL-6 expression in H. pylori-infected and uninfected gastric mucosa and elucidate the implication in the pathogenesis of H. pylori-associated gastritis in human.Objectives:The current study aimed to determine mucosal IL-6 mRNA expression level and their correlation with virulence factors and the grade of chronic gastritis among H. pylori infected patients with chronic gastritis from Shahrekord, Iran.Patients and Methods:Mucosal IL-6 mRNA levels was measured by real-time PCR using endoscopic biopsies taken from the gastric antrum of 58 subjects infected with H. pylori and 44 uninfected subjects. Presence of vacA and cagA virulence factors was evaluated using PCR.Results:The IL-6 mRNA expression levels were significantly more elevated in H. pylori-positive patients than uninfected individuals and expression of this cytokine was independent from the virulence factors. There was a correlation between IL-6 expression level and the grade of chronic gastritis.Conclusions:Enhanced induction of IL-6 may be involved in the pathogenesis of H. pylori-associated gastritis.

Propolis: The future therapy against Helicobacter pylori-mediated gastrointestinal diseases

“…Heat shock protein 60 (Hp-HSP60) and urease produced by H. pylori are potent triggers of both IL-6 synthesis and release from macrophages of human mucosa (Gobert et al, 2004;Harris et al, 1998). The mRNA level of IL-6 and the degree of H. pylori-associated chronic gastritis are positively correlated (Azadegan-Dehkordi et al, 2015). IL-6 in combination with TGF-b triggers the differentiation of naive CD4+ T-cells into Th17 cells.…”