Correlation between mouse age and human age in anti-tumor research: Significance and method establishment

Wang, Shuo; Lai, Xiaoxue; Deng, Yihui; Song, Yanzhi

doi:10.1016/j.lfs.2019.117242

Cited by 111 publications

(84 citation statements)

References 62 publications

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“…Hence, the maximum possible number of animals per time should be used in the analysis to limit the variability of results. Moreover, the age of mice used in this study (6–8 weeks) resembles the puberty stage in humans 63 , 64 , yet clinically pain is more common in elderly patients 65 …”

Section: Discussionmentioning

confidence: 99%

Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain

Hasriadi

Wasana

Vajragupta

et al. 2021

Sci Rep

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The failure to develop analgesic drugs is attributed not only to the complex and diverse pathophysiology of pain in humans but also to the poor experimental design and poor preclinical assessment of pain. Although considerable efforts have been devoted to overcoming the relevant problems, many features of the behavioral pain assessment remain to be characterized. For example, a decreased locomotor activity as a common presentation of pain-like behavior has yet to be described. Studies on mice experimentally induced with carrageenan have provided opportunities to explore pain-related behaviors in automated home-cage monitoring. Through this approach, the locomotor activities of mice with carrageenan-induced inflammatory pain can be precisely and objectively captured. Here, we found that the mobile behaviors of mice reduced, and their immobility increased, indicating that carrageenan induction in mice caused a significant decrease in locomotor activity. These non-reflexive pain behaviors were strongly correlated with the reflexive pain behaviors measured via von Frey and plantar tests. Furthermore, the pharmacological intervention using indomethacin improved the locomotor activity of mice with carrageenan-induced pain. Thus, the analysis of the locomotor activity in automated home-cage monitoring is useful for studying the behavioral analgesia and the pharmacological screening of analgesic drugs. The combined evaluation of reflexive and non-reflexive pain behaviors enhances the translational utility of preclinical pain research in rodents.

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Section: Discussionmentioning

confidence: 99%

Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain

Hasriadi

Wasana

Vajragupta

et al. 2021

Sci Rep

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“…Herein, infants and adult mice were used: the mice in the infant group weighed 10-12 g and were 3-4 weeks old, while mice in the adult group weighed 38-56 g and were 8-12 weeks old. According to the literature, infant mice had not entered puberty (equivalent to children), since in this stage of development 1 human year corresponds to 56.77 mice days [67,68], meaning that at the beginning of the administrations, the age of infant mice corresponded to roughly 135-180 human days. On the other hand, the mice at 8 to 12 weeks old clearly reached the young adulthood phase [67,68], since adult mice reach sexual maturity on average at 10 weeks, while in humans, this stage corresponds to an average age of 20 years [67,68].…”

Section: Experimental Protocolmentioning

confidence: 99%

Inflammation as a Possible Trigger for Mitoxantrone-Induced Cardiotoxicity: An In Vivo Study in Adult and Infant Mice

et al. 2021

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Mitoxantrone (MTX) is a pharmaceutical drug used in the treatment of several cancers and refractory multiple sclerosis (MS). Despite its therapeutic value, adverse effects may be severe, namely the frequently reported cardiotoxicity, whose mechanisms need further research. This work aimed to assess if inflammation or oxidative stress-related pathways participate in the cardiotoxicity of MTX, using the mouse as an animal model, at two different age periods (infant or adult mice) using two therapeutic relevant cumulative doses. Histopathology findings showed that MTX caused higher cardiac toxicity in adults. In MTX-treated adults, at the highest dose, noradrenaline cardiac levels decreased, whereas at the lowest cumulative dose, protein carbonylation increased and the expression of nuclear factor kappa B (NF-κB) p65 subunit and of M1 macrophage marker increased. Moreover, MTX-treated adult mice had enhanced expression of NF-κB p52 and tumour necrosis factor (TNF-α), while decreasing interleukin-6 (IL-6). Moreover, while catalase expression significantly increased in both adult and infant mice treated with the lowest MTX cumulative dose, the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glutathione peroxidase only significantly increased in infant animals. Nevertheless, the ratio of GAPDH to ATP synthase subunit beta decreased in adult animals. In conclusion, clinically relevant doses of MTX caused dissimilar responses in adult and infant mice, being that inflammation may be an important trigger to MTX-induced cardiotoxicity.

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“…In the present study, the auditory function of SAMP8 mice was analyzed at the age of 6 weeks (juvenile), 12 weeks (young adult), and 24 weeks (adult; Wang et al, 2020 ) by measuring auditory brainstem response (ABR) thresholds. We observed a bifurcation into two subgroups, i.e., in lower and upper quartiles with respect to different progressions of age-related ABR threshold increase.…”

Section: Introductionmentioning

confidence: 99%

Auditory Threshold Variability in the SAMP8 Mouse Model of Age-Related Hearing Loss: Functional Loss and Phenotypic Change Precede Outer Hair Cell Loss

Pinheiro

Adel

Knipper

et al. 2021

Front. Aging Neurosci.

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Age-related hearing loss (ARHL) is the most common sensory deficit in aging society, which is accompanied by increased speech discrimination difficulties in noisy environments, social isolation, and cognitive decline. The audiometric degree of ARHL is largely correlated with sensory hair cell loss in addition to age-related factors not captured by histopathological analysis of the human cochlea. Previous studies have identified the senescence-accelerated mouse prone strain 8 (SAMP8) as a model for studying ARHL and age-related modifications of the cochlear redox environment. However, the SAMP8 population exhibits a large variability in auditory function decline over age, whose underlying cause remains unknown. In this study, we analyzed auditory function of SAMP8 mice by measuring auditory brainstem response (ABR) thresholds at the age of 6 weeks (juvenile), 12 weeks (young adult), and 24 weeks (adult). Consistent with previous studies, SAMP8 mice exhibit an early progressive, age-related decline of hearing acuity. However, a spatiotemporal cytohistological analysis showed that the significant increase in threshold variability was not concurrently reflected in outer hair cell (OHC) loss observed in the lower and upper quartiles of the ABR threshold distributions over age. This functional loss was found to precede OHC loss suggesting that age-related phenotypic changes may be contributing factors not represented in cytohistological analysis. The expression of potassium channels KCNQ4 (KV7.4), which mediates the current IK,n crucial for the maintenance of OHC membrane potential, and KCNQ1 (KV7.1), which is an essential component in potassium circulation and secretion into the endolymph generating the endocochlear potential, showed differences between these quartiles and age groups. This suggests that phenotypic changes in OHCs or the stria vascularis due to variable oxidative deficiencies in individual mice may be predictors of the observed threshold variability in SAMP8 mice and their progressive ARHL. In future studies, further phenotypic predictors affected by accumulated metabolic challenges over age need to be investigated as potentially underlying causes of ARHL preceding irreversible OHC loss in the SAMP8 mouse model.

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Correlation between mouse age and human age in anti-tumor research: Significance and method establishment

Cited by 111 publications

References 62 publications

Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain

Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain

Inflammation as a Possible Trigger for Mitoxantrone-Induced Cardiotoxicity: An In Vivo Study in Adult and Infant Mice

Auditory Threshold Variability in the SAMP8 Mouse Model of Age-Related Hearing Loss: Functional Loss and Phenotypic Change Precede Outer Hair Cell Loss

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