Correlation between microsomal ()-ATPase activity and [3H] ouabain binding to heart tissue homogenates

Gelbart, Alex; Goldman, Robert H.

doi:10.1016/0005-2744(77)90302-3

Cited by 22 publications

(7 citation statements)

References 10 publications

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“…Despite the indirect nature of ATPase assay, the number of ouabain binding sites assessed by specific binding of ouabain has been repeatedly shown to correlate with the activity of this enzyme (Schmalzing et al 1981 ;Gelbert et al 1977). In accordance with the present data, the Vmax value of ouabain (Na, K, ATPase-mediated) rubidium uptake by erythrocytes from hyperthyroid patients was found to be lower than the value in normals in our preliminary experiment (unpublished observation).…”

Section: Discussionsupporting

confidence: 74%

See 1 more Smart Citation

Erythrocyte ouabain binding capacity as a possible cellular index of hyperthyroid status.

Suzuki¹,

Kadowaki²,

Sekimizu³

et al. 1983

Endocrinol Japon

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Section: Discussionsupporting

confidence: 74%

“…Other workers (Schmalzing et al 1980;Gelbert and Goldman 1977) Their thyroid status were evaluated by the clinical signs and symptoms and also by the results of T3 and T4 radioimmunoassays.…”

mentioning

confidence: 99%

Erythrocyte ouabain binding capacity as a possible cellular index of hyperthyroid status.

Suzuki¹,

Kadowaki²,

Sekimizu³

et al. 1983

Endocrinol Japon

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“…Although an elevated cholesterol con tent or an increased C/P ratio was reported to inhibit the Na+,K+-ATPase activity [19], no good correlation of age-related changes in the enzyme activity to the C/P ratio was observed in our study [7], Changes in the amount of the enzyme protein were examined, as the second mech anism underlying decreased ion-pumping ac tivity. The ouabain-sensitive enzyme mole cules were determined as ouabain binding sites [20] as shown in table 2. Binding sites decrease significantly in late senescence.…”

Section: Discussionmentioning

confidence: 99%

Synaptic Membrane Aging in the Central Nervous System

Ando

Tanaka²

1990

Gerontology

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Membrane potentials involved in neuronal excitability, transmitter uptake and so on were determined using synaptosomes from different age groups of mice. Synaptosomal resting membrane potential was found to decrease significantly in senescence. Concomi-tantly decreased activity of an electrogenic enzyme, Na⁺, K⁺-ATPase, was interpreted by two mechanisms. In senescence, the decreased content of phosphatidylcholine seemed responsible in part for decreased enzyme activity due to the modified lipid microenvironment. In the late stages of senescence, decreased enzyme content may rather cause reduction of enzyme activity, leading to a less negative set point of the membrane potential.

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“…The maximal ouabain binding capacity of cells represents the number, and the activity as well, of Na, K-ATPase units (Gelbart and Goldman 1977;Schmalzing et al 1981). By use of [3H]-ouabain binding assay introduced by De Luise et al (1980), we found that the maximal ouabain binding capacity in erythrocytes from mature rabbits was significantly less as compared with that in young rabbits, and that ouabain binding capacity in each young rabbit decreased after 8 weeks of aging.…”

Section: Discussionmentioning

confidence: 61%