Correlation between <i>in vitro</i> Anaphylactic Histamine Release from Tissues and Reagin Titer in Rat

Abstract

The magnitude of antigen-induced histamine release from actively sensitized rat tissues in vitro was compared with the amount of specific reagin in the serum. There was no quantitative correlation between either, suggesting that the antigen-induced histamine release from sensitized tissues might depend on the ability of sensitized target cells to release histamine. In addition, the magnitude of antigen-induced histamine release was different in each tissue of sensitized rats. This also suggested that there might be some differences in the ability of sensitized target cells in each tissue to release histamine by antigen.

Preferential Synthesis of IgE Reaginic Antibodies in Rats Immunized with Alum-Adsorbed Antigens

“…Similar com parative results were found in animals im munized with DWSR. These observations seem to be similar to previous reports in the literature [12,19], Results of our experiments strongly sug gest that the reaginic response to alum-ad sorbed OA, DWSR, and DWST was due en tirely to IgE antibody. There was no evi dence of heat-stable IgGa antibody to OA, DWSR, and DWST in the sera of these rats for as long as 35 days after immunization.…”

Relationship between Tissue Sensitization and IgE Antibody Production in Rats Infected with the Nematode, <i>Nippostrongylus brasiliensis</i>

“…The relationship between the responsiveness of tis sues to antigenic challenge and levels of circulating IgE antibody has been extensively studied [10,19,28,38], although no good correlation has been identified. To study tissue sensitization and its relationship to lo cal reagin production, we used rats infected with the nematode Nippostrongylus brasiliensis.…”

Rosette-Forming Mast Cells in Rat Anaphylaxis

“…However, there is no correlation between mast cell ro settes and the reaginic content of sera, esti mated by PCA reactions. This discrepancy between cell-bound and circulating IgE has been reported and well documented in the rat [Jarrett and Stewart, 1973;Yamamoto et al, 1974] as well as in man [Aas and Jo hansson, 1971;Berg et al, 1971;Wilson et al, 1972]. This fact is of interest, inasmuch as clinical allergy mainly depends on cellbound reagins.…”