Correlation between left ventricular mass and urinary sodium excretion in specific genotypes of CYP11B2

Abstract

The -344CC or intron 2 conversion (-/-) genotype in CYP11B2 may be a risk factor for developing sodium-sensitive cardiac hypertrophy. Ethnic differences in the distribution of CYP11B2 genotypes combined with differences in salt intake might account for inconsistencies between previous reports.

Aldosterone synthase (CYP11B2) C-344T polymorphism affects the association of age-related changes of the serum C-reactive protein

“…Allele and genotype frequencies were analyzed using the genecounting method, and the Hardy-Weinberg equilibrium, checked by the w 2 -test, was considered to be maintained as described previously. 13,17 First, statistical significance was determined by one-way ANOVA (analysis of variance) or by the w 2 -test. In the case of statistically significant relations, the Bonferroni analysis was applied to assess the relationship between categories.…”

“…In addition, two previous studies reported that the increase in PWV with increasing age independently of BP is most prominent in subjects with the CC genotype, 34 and that the CC genotype might be an independent risk factor for the development of sodium-sensitive cardiac hypertrophy. 17 Taken together, it is suggested that subjects with the CC genotype might have an amplification mechanism promoting the atherosclerotic process through inflammation, independently of BP.…”

“…3 On the other hand, there is positive association between left ventricular mass and sodium intake in subjects with the CC genotype. 17 Accordingly, it is speculated that strong binding between the CYP11B2 promoter and SF-1 may inhibit prompt suppression of CYP11B2 by sodium loading in the CC genotype, resulting in an inappropriately higher level of aldosterone and sodium. It is reported that this population, as well as native Japanese, consumed a relatively high level of dietary salt, 29,35 and thus high sodium intake might induce or exacerbate vascular inflammation through inappropriate suppression of aldosterone only in Japanese Americans with the CC genotype.…”

“…Genotyping of the CYP11B2 C-344T polymorphism, rs1799998, was performed as described previously. 17 It was amplified using EX Taq DNA polymerase (TaKaRa, Osaka, Japan) and the following primer set: forward primer 5¢-CAT TTG TCA AAA CCC ACA GC-3¢ and reverse primer 5¢-TCA CGT CAT GGG AGA ACT GG-3¢. The PCR conditions were as follows: initial denaturation at 96 1C for …”

“…3 An overview of published studies suggests a higher frequency of hypertension or increase in blood pressure (BP) in subjects with the T allele or TT genotype as compared with that in subjects with the C allele or CC genotype, [4][5][6][7][8][9][10] although several studies have reported the opposite association 11,12 or the absence of any association. [13][14][15][16][17][18][19] Thus, the associations remain controversial. Recently, two interesting studies have suggested a key indicator that may resolve the association.…”