Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies

Miyahara, Glauco Issamu; Simonato, Lorenzo; Mattar, Neivio José; Camilo, Deolino João; Biasoli, Éder Ricardo

doi:10.1590/s0074-02762011000200008

Cited by 15 publications

(17 citation statements)

References 13 publications

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“…However, our data differs from other studies performed in Brazil that report HPV rates ranging from 0% to 30% in oral lesions 1 , 14 , 22 . In São Paulo state, Rivero and Nunes 22 (2006) could not identify HPV in any of the 40 samples with SCC.…”

Section: Discussioncontrasting

confidence: 99%

High prevalence of human papillomavirus (HPV) in oral mucosal lesions of patients at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Northeastern Brazil

et al. 2017

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The role of human papillomavirus (HPV) in oral carcinogenesis is still controversial as detection rates of the virus in oral cavity reported in the literature varies greatly. Objective The aim of this study was to evaluate the frequency of HPV infection and its genotypes in patients with oral lesions at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Brazil.Material and Methods We conducted a molecular study with 21 patients (15 females) aged from two to 83 years with clinically detectable oral lesions. Samples were collected through exfoliation of lesions and HPV-DNA was identified using MY09/11 and GP5+/6+ primers. Genotyping was performed by multiplex PCR.Results Benign, premalignant and malignant lesions were diagnosed by histopathology. HPV was detected in 17 samples. Of these, HPV-6 was detected in 10 samples, HPV-18 in four and HPV-16 in one sample. When samples were categorized by lesion types, HPV was detected in two papilloma cases (2/3), five carcinomas (5/6), one hyperplasia (1/1) and nine dysplasia cases (9/11).Conclusion Unlike other studies in the literature, we reported high occurrence of HPV in oral lesions. Further studies are required to enhance the comprehension of natural history of oral lesions.

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Section: Discussioncontrasting

confidence: 99%

High prevalence of human papillomavirus (HPV) in oral mucosal lesions of patients at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Northeastern Brazil

et al. 2017

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“…Miyahara et al . (2011)[ 26 ] compared the histopathological analysis with PCR to predict the presence of HPV infection in OSCC biopsy tissues and found that the presence of koilocytes is unreliable for the detection of HPV presence in oral and oropharyngeal SCC.…”

Section: Discussionmentioning

confidence: 99%

HPV involvement in OSCC: Correlation of PCR results with light microscopic features

Khangura

Sengupta²,

Sircar

et al. 2013

J Oral Maxillofac Pathol

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Objectives:The study evaluated pathognomic histopathological features with the help of light microscopy for detecting the integration of human papillomavirus (HPV) (type 16 and 18) in oral squamous cell carcinoma (OSCC).Materials and Methods:Forty-five histopathologically diagnosed cases of OSCC were evaluated for the presence of E6/E7 protein of HPV (16 + 18) with the help of nested multiplex polymerase chain reaction. Both HPV-positive and -negative cases were evaluated for four histological features: Koilocytes, dyskeratosis, invasion, and alteration of collagen.Results:Fischer's exact test showed significant difference (P < 0.01%) for the presence of koilocytes and dyskeratosis, whereas no difference was observed for invasion and alteration in collagen between HPV-positive and -negative OSCC.Conclusion:The presence of koilocytes and dyskeratosis at light microscopic level can be used as a marker for the presence of HPV (type 16 and 18) in OSCC.

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“…Meanwhile, real-time PCR results showed HR-HPV DNA was positive in women with normal cytology that similar to Arora et al [12], suggested that the cases who are HPV positive for any of the high risk types but have concurrently negative Pap smear or no visible lesions should be followed up more frequently than the HPV negative cases as they have a substantially greater risk of developing an abnormal smear. Since morphological changes on cytology such as koilocytosis are not specific for oncogenic HPV [19], our finding showed that eight women had all criteria for HPV infection (such as koilocytosis) was HR-HPV DNA negative. Prevalence of HR-HPV DNA was more frequency in HG, thus a distinction between the pathological diagnoses of HG-CIN (CIN II/III) may be important in future clinical studies of real-time PCR HR-HPV screening and the prevention of cervical cancer.…”

Section: Discussionmentioning

confidence: 69%

Screening for High-Risk Human Papillomavirus by Real-Time PCR and Its Association With Cytological Abnormalities of Pap Smears in Baghdad

DS¹,

Hy²,

FG³

2012

Int J of Micr Res

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Abstract-Human Papillomaviruses (HPVs) are known to be etiological agents of cervical cancer and have been found in 99.7% of women with high-grade (HG) cervical intraepithelial neoplasia (CIN) pre-cancer. Testing of high-risk HPV (HR-HPV) has been proposed as a way of improving cervical screening, especially for women with low-grade (LG) Papanicolaou (Pap) smears. Therefore, the present study aimed to investigate the diagnostic correlation of real-time PCR technique with Pap smear results for 13 genotypes of HR-HPV in 415 of suspected women. Pap smear results showed the suspected women with abnormal and normal cytological changes were 79.3% (329/415) and 20.7% (86/415), respectively. In contrast, real-time PCR results showed that women with or without abnormal changes had HR-HPV DNA positive were 50.5% (166/329) and 10.5% (9/86), respectively. Thus, Pap smear results had no sensitive to predicate risky for LG-CIN women were observed. According to the real-time PCR results, the incident of HG or cancer was more associated with HR-HPV. Our findings indicated the real-time PCR test with the cytological test as a routine practice for women with LG-CIN reduces the incidence of HG or cancer. After that, the prospective studies should be appropriated to determine HR-HPV genotypes and viral load (copies/cell) with realtime PCR test as predicate risk-factor.

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Correlation between koilocytes and human papillomavirus detection by PCR in oral and oropharynx squamous cell carcinoma biopsies

Cited by 15 publications

References 13 publications

High prevalence of human papillomavirus (HPV) in oral mucosal lesions of patients at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Northeastern Brazil

High prevalence of human papillomavirus (HPV) in oral mucosal lesions of patients at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Northeastern Brazil

HPV involvement in OSCC: Correlation of PCR results with light microscopic features

Screening for High-Risk Human Papillomavirus by Real-Time PCR and Its Association With Cytological Abnormalities of Pap Smears in Baghdad

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