Correlation between KLK6 expression and the clinicopathological features of glioma

Lou, Jiangtao; Si, Haiyan; Chen, Yingjian; Sun, Xuemei; Zhang, Hua; Ai-jun, Niu; Hu, Chengjin

doi:10.5114/wo.2014.44628

Cited by 5 publications

(5 citation statements)

References 23 publications

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“…KLK6 (zyme/protease M/neurosin) appears to be down-regulated in serum and tissues of Alzheimer's disease patients and may be involved in amyloid metabolism [143, 144]. Moreover, recent studies also correlate lower expression of KLK6 with increased glioma growth [145]. Whereas in case of glioblastoma, a highly malignant type of glioma tumor with different physiology then glioma, KLK6 is responsible for reduction in cell sensitivity to cytotoxic agents, including staurosporine and cisplatin, and current standard treatments of radiotherapy, or temozolomide, alone or in combination.…”

Section: Physiological Function Of Klksmentioning

confidence: 99%

Kallikreins – The melting pot of activity and function

et al. 2016

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The human tissue kallikrein and kallikrein-related peptidases (KLKs), encoded by the largest contiguous cluster of protease genes in the human genome, are secreted serine proteases with diverse expression patterns and physiological roles. Because of the broad spectrum of processes that are modulated by kallikreins, these proteases are the subject of extensive investigations. This review brings together basic information about the biochemical properties affecting enzymatic activity, with highlights on post-translational modifications, especially glycosylation. Additionally, we present the current state of knowledge regarding the physiological functions of KLKs in major human organs and outline recent discoveries pertinent to the involvement of kallikreins in cell signaling and in viral infections. Despite the current depth of knowledge of these enzymes, many questions regarding the roles of kallikreins in health and disease remain unanswered.

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Section: Physiological Function Of Klksmentioning

confidence: 99%

Kallikreins – The melting pot of activity and function

et al. 2016

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“…Our results revealed that MRGs, including GLDC and KLK6 may affect DUs by mediating angiogenesis and re‐epithelization. GLDC is a pivotal enzyme in the glycine lysis system, whose expression is downregulated in gastric cancer cell lines and tissues, and the proliferation and migration of gastric cancer cells can be inhibited by methylation of GLDC (Min et al, 2016); 41 KLK6 is a secreted serine protease that downregulates mRNA expression in gliomas and can inhibit tumor growth (Lou et al, 2014) 42 . The suggestion that GLDC and KLK6 may inhibit wound healing of DUs is consistent with our findings.…”

Section: Discussionmentioning

confidence: 99%

Metabolism‐related biomarkers, molecular classification, and immune infiltration in diabetic ulcers with validation

Zhang

Jiang

et al. 2023

International Wound Journal

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Diabetes mellitus (DM) can lead to diabetic ulcers (DUs), which are the most severe complications. Due to the need for more accurate patient classifications and diagnostic models, treatment and management strategies for DU patients still need improvement. The difficulty of diabetic wound healing is caused closely related to biological metabolism and immune chemotaxis reaction dysfunction. Therefore, the purpose of our study is to identify metabolic biomarkers in patients with DU and construct a molecular subtype‐specific prognostic model that is highly accurate and robust. RNA‐sequencing data for DU samples were obtained from the Gene Expression Omnibus (GEO) database. DU patients and normal individuals were compared regarding the expression of metabolism‐related genes (MRGs). Then, a novel diagnostic model based on MRGs was constructed with the random forest algorithm, and classification performance was evaluated utilizing receiver operating characteristic (ROC) analysis. The biological functions of MRGs‐based subtypes were investigated using consensus clustering analysis. A principal component analysis (PCA) was conducted to determine whether MRGs could distinguish between subtypes. We also examined the correlation between MRGs and immune infiltration. Lastly, qRT‐PCR was utilized to validate the expression of the hub MRGs with clinical validations and animal experimentations. Firstly, 8 metabolism‐related hub genes were obtained by random forest algorithm, which could distinguish the DUs from normal samples validated by the ROC curves. Secondly, DU samples could be consensus clustered into three molecular classifications by MRGs, verified by PCA analysis. Thirdly, associations between MRGs and immune infiltration were confirmed, with LYN and Type 1 helper cell significantly positively correlated; RHOH and TGF‐β family remarkably negatively correlated. Finally, clinical validations and animal experiments of DU skin tissue samples showed that the expressions of metabolic hub genes in the DU groups were considerably upregulated, including GLDC, GALNT6, RHOH, XDH, MMP12, KLK6, LYN, and CFB. The current study proposed an auxiliary MRGs‐based DUs model while proposing MRGs‐based molecular clustering and confirmed the association with immune infiltration, facilitating the diagnosis and management of DU patients and designing individualized treatment plans.

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“…Human KLKs take part in multiple physiological processes, including skin desquamation, tooth enamel formation, kidney and brain function, and synaptic neural plasticity ( 66–75 ). Also, recent studies demonstrate a role for some KLKs during viral infections.…”

Section: Discussionmentioning

confidence: 99%

Kallikrein 13: a new player in coronaviral infections

Milewska

Falkowski

Kalińska

et al. 2020

Preprint

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35Human coronavirus HKU1 (HCoV-HKU1) is associated with respiratory disease and is 36 prevalent worldwide, but in vitro model for virus replication is lacking. Interaction between the 37 coronaviral spike (S) protein and its receptor is the major determinant of virus tissue and host 38 specificity, but virus entry is a complex process requiring a concerted action of multiple cellular 39 elements. Here, we show that KLK13 is required for the infection of the human respiratory 40 epithelium and is sufficient to mediate the entry of HCoV-HKU1 to non-permissive RD cells. 41 We also demonstrated HCoV-HKU1 S protein cleavage by KLK13 in the S1/S2 region, proving 42 that KLK13 is the priming enzyme for this virus. Summarizing, we show for the first time that 43 protease distribution and specificity predetermines the tissue and cell specificity of the virus 44 and may also regulate interspecies transmission. It is also of importance that presented data may 45 be relevant for the emerging coronaviruses, including SARS-CoV-2 and may help to understand 46 the differences in their zoonotic potential. : bioRxiv preprint 48Coronaviruses are the largest group within the order Nidovirales. Mainly, they cause 49 respiratory and enteric diseases in humans and animals, but some can cause more serious 50 conditions such as hepatitis, peritonitis, or neurological disease. Seven coronaviruses infect 51 humans, four of which (human coronavirus [HCoV]-229E, HCoV-NL63, HCoV-OC43, and 52 HCoV-HKU1) cause relatively mild upper and lower respiratory tract disease and two (SARS-53 CoV and MERS-CoV) are associated with severe, life-threatening respiratory infections and 54 multiorgan failure (1-6). Furthermore, in December 2019 a novel coronavirus SARS-CoV-2 55 emerged in Hubei province, China, causing pneumonia. To date, almost 90,000 cases were 56 identified and 3,000 patients died worldwide. 57 Coronaviral infection is initiated by interaction between the trimeric spike (S) protein 58 and its receptor, which is expressed on the surface of the susceptible cell. A number of adhesion 59 and entry receptors have been described for coronaviruses. For example, HCoV-229E (similar 60 to many other alphacoronaviruses) utilizes aminopeptidase N (APN) as the primary entry port 61 (7). Surprisingly, its cousin HCoV-NL63 shares receptor specificity with the evolutionarily 62 distant SARS-CoV and SARS-CoV-2: all hijack angiotensin-converting enzyme 2 (ACE2) (8-63 11). HCoV-NL63 was also shown to use heparan sulfate as a primary attachment site (12-14). 64 A very different receptor is recognized by MERS-CoV, which binds to dipeptidyl-peptidase 4 65 (DPP4) (9, 15, 16). Another betacoronavirus, HCoV-OC43, binds to N-acetyl-9-O-66 acetylneuraminic acid (17, 18). HCoV-HKU1 remains the great unknown because its cellular 67 receptor has not been identified and all efforts to culture the virus in vitro have failed.68 HCoV-HKU1 was identified in Hong Kong in 2004. The virus was present in a sample 69 obtained from an elderly patient with severe pneumonia (...

show abstract

Correlation between KLK6 expression and the clinicopathological features of glioma

Cited by 5 publications

References 23 publications

Kallikreins – The melting pot of activity and function

Kallikreins – The melting pot of activity and function

Metabolism‐related biomarkers, molecular classification, and immune infiltration in diabetic ulcers with validation

Kallikrein 13: a new player in coronaviral infections

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