Correlation Between Kidney Function and Intestinal Biological Characteristics of Adenine and Folium SennaeInduced Diarrhea Model in Mice

Abstract

Background: This study investigated the correlation among kidney function, intestinal enzyme activities, and microbial activity of adenine and Folium sennae -induced diarrhea model in mice, which provided a basis for clinical treatment of kidney-intestinal correlation. Methods: We performed different doses of adenine combined with Folium sennae intragastric administration to establish the animal model of diarrhea. We assessed thymus and spleen indexes, serum creatinine, urea nitrogen and uric acid contents, intestinal contents and mucosal enzyme activities, and microbial activity. Results: After modeling, mice presented increased serum creatinine and decreased urea nitrogen. Uric acid showed different changes in the different model groups. The thymus index in the model mice was trending downward, whereas the spleen index was the opposite. Moreover, model mice induced a non-significant increase in xylanase activity of the intestinal contents and mucosa compared to the control performance. Sucrase content of the intestinal contents increased considerably in the model groups but decreased in the intestinal mucosa. Lactase and amylase induced different trends in the different modeling methods. As well, the microbial activity of intestinal contents increased significantly, while that of intestinal mucosa decreased. Conclusion: Adenine combined with Folium sennae successfully replicated diarrhea in mice models. Using 50 mg/ (kg/day) adenine for 14 days in combination with 10 g/(kg/day) Folium sennae decoction for 7 days caused kidney function injury in diarrhea mice. In addition, kidney function injury was accompanied by changing in intestinal functional enzyme activity and microbial activity.

Simo decoction curing spleen deficiency constipation was associated with brain-bacteria-gut axis by intestinal mucosal microbiota

“…In our animal experiment, spleen deficiency constipation could cause weight loss, while the weight increased significantly after SMD intervention, indicating that SMD could help weight recovery, this is probably because SMD has the function of invigorating spleen and stomach, increasing the appetite, and improving its digestion and absorption functions ( Liu, 2014 ). The thymus and spleen are important for establishing the body’s immune function ( Li et al, 2022c ). Therefore, after the intervention of SMD, we examined the spleen and thymus indices.…”

Dose of sucrose affects the efficacy of Qiweibaizhu powder on antibiotic-associated diarrhea: Association with intestinal mucosal microbiota, short-chain fatty acids, IL-17, and MUC2

“…Preparation of FDA stock solution and FDA reaction solution: 2 mg of FDA is mixed with 1 mL of acetone to produce 2 mg/mL of FDA stock solution, reserved at 2-8°C and protected from light, added FDA stock solution to sterilized PBS (pH = 7.6) so that the final concentration of FDA reaction solution is 10 μg/mL ( Guo et al, 2022 ; Li X. et al, 2022 ).…”

“…Experimental group: Added 2 mL FDA reaction solution and 50 μL sample into a dry sterilized tube, and the mixture was incubated at 24°C for 90 min, subsequently removed, and added 2 mL acetone was to terminate the reaction. Finally, all samples were determined three times in parallel by UV spectrophotometer at 490 nm, and the microbial activity per unit mass of the sample is expressed as A 490 value ( Guo et al, 2022 ; Li X. et al, 2022 ).…”

Gut-Kidney Impairment Process of Adenine Combined with Folium sennae-Induced Diarrhea: Association with Interactions between Lactobacillus intestinalis, Bacteroides acidifaciens and Acetic Acid, Inflammation, and Kidney Function

“…Our research group’s preliminary research presented that mice showed obvious diarrhea symptoms and caused gut microbiota disorders after the Folium sennae modeling [ 20 ]. Furthermore, we compared the effects of adenine combined with Folium sennae at different doses and days, on the kidney and gut functions in mice and presented that adenine (50 mg/(kg·d), gavaged for 14 days), combined with Folium sennae (10 g/(kg·d), gavaged for 7 days,) significantly caused impairment of the kidney and gut function in mice [ 21 ]. Subsequently, we have successfully constructed and validated a mouse model of diarrhea using the same modeling method as described above, thus confirming the reliability of the model [ 22 ].…”