Correlation between Increased Serum sFas Levels and Microalbuminuria in Type 1 Diabetic Patients

Abstract

Objective: The aim of this study was to elucidate if apoptosis dysregulation is present in type 1 diabetic patients with microalbuminuria. Subjects and Methods: The following variables were determined in 29 type 1 diabetic patients: the duration of diabetes, soluble Fas (sFas), Bcl-2, hemoglobin A_1c levels, glomerular filtration rate (GFR) and microalbuminuria, using the urine albumin to urine creatinine ratio (ACR). Age and gender were assessed and patients were categorized into two groups, according to their ACR: the microalbuminuric (MA) group with an ACR ≧30 mg/g, and the normoalbuminuric (NA) group with an ACR <30 mg/g. Results: The differences between the two groups regarding sFas, Bcl-2 and GFR were not statistically significant. However, in the MA group, a significant positive relationship between sFas and ACR was observed (r = 0.736, p = 0.015). Dividing patients into two subgroups – mild versus severe (ACR ≧150 mg/g) microalbuminuric patients – significant differences in sFas (60.4 vs. 87.2 pg/ml; p = 0.047) and GFR (113 vs. 69.5 ml min^–1 1.73 m^–2; p = 0.021) were observed, whereas in Bcl-2, the difference was not significant (77.96 vs. 71.13 ng/ml). Conclusions: At the early stages of diabetic nephropathy in type 1 diabetic patients, there seems to be a dysregulation of apoptosis, as expressed by enhanced sFas levels, leading to the speculation that the prevalence of antiapoptotic mechanisms (sFas) may promote mesangial proliferation.

Leptin receptor interacts with rat chromosome 1 to regulate renal disease traits

“…Several genes in the donor region are functional candidates known to influence renal function and UAE including FAS (Tnfrsf6) (17) and discoidin domain receptor tyrosine kinase 1 (Ddr1) (5,8). Studies with recombinant congenics will be needed to reduce the number of positional candidates.…”

“…QTL analyses were performed using the R/qtl package from the R: Language and Environment program in 248 ZK.BN-Chr1 congenic F2 males (2,17). The genetic map for rat chromosome 1 was developed by first computing recombination frequencies between all markers using the est.rf function, then the construction of a genetic map was performed with the est.map function.…”

Extracellular BCL2 Proteins Are Danger-Associated Molecular Patterns That Reduce Tissue Damage in Murine Models of Ischemia-Reperfusion Injury

“…S7). More importantly, soluble BCL2 protein has been detected in serum in a variety of disorders [51]–[55] and cerebrospinal fluid in traumatic brain injury [56], [57]. Notably, in children following head trauma increased levels of soluble BCL2 protein in spinal fluid correlated with improved survival [57].…”

Serum levels of sFas and sFasL in subjects with type 2 diabetes — the impact of arterial hypertension

“…Another study showed increased sFas values in subjects with type 2 DM, with signifi cantly higher levels in subjects with longer duration of diabetes [16]. Protopsaltis et al have observed positive correlation of sFas with microalbuminuria [17] and diabetic nephropathy [18][19][20]. Higher values of serum sFas have been found in patients in the advanced stage of renal disease as compared to the early stages [20].…”