Correlation between in vivo hemolysis and the amount of red cell‐bound IgG measured by flow cytometry

Garratty, George; Nance, Sandra

doi:10.1046/j.1537-2995.1990.30790385519.x

Cited by 75 publications

(65 citation statements)

References 9 publications

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“…With two exceptions, the results of van der Meulen et al [4] indicated that there was a distinct difference in the number of RBC-bound IgG1 molecules in patients with and without IHA. Garratty and Nance [5] later studied 103 DAT-positive patients and blood donors with and without IHA, but were unable to confirm the distinct difference seen by van der Meulen et al [4]. Using FITC antiIgG to study the RBCs, Garratty and Nance [5] were able to show that, although the mean percentage of cells with increased fluorescence was higher in the group with IHA, there was considerable overlap in the results from both groups (with and without IHA).…”

Section: Detection and Quantitation Of Globulins Red Blood Cell-boundmentioning

confidence: 92%

Flow Cytofluorometric Analysis in Red Blood Cell Immunology

Arndt¹,

Garratty²

2004

Transfus Med Hemother

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Flow cytofluorometry (FC) is primarily used in the clinical laboratory for the analysis of white blood cells but also has applications in the study of red blood cell (RBC) immunology. When studying RBCs by FC it is important to avoid agglutination of the RBCs, which ironically is the endpoint for most immunohematologic assays. When studying low levels of RBC-bound antibody or RBC antigens with low copy number, background noise due to autofluorescence and nonspecific binding need to be taken into account. It is also important to ensure that reagents (e.g. antisera) used by FC have been standardized for use by FC. Applications of FC in RBC immunology include i) detection and quantitation of globulin (RBC-bound or free in serum), ii) detection and quantitation of RBC antigens, and iii) detection and quantitation of mixed cell populations. FC can be used to differentiate the amount of IgG on strongly IgG-coated RBCs, as well as to detect small amounts of RBC-bound IgG. FC has been used to quantitate antibody that is free in serum, e.g. anti-D in a pregnant woman or in immune globulin preparations. Many RBC antigens have been studied by FC to determine the number of antigen sites, the density distribution, and phenotype-related differences (e.g. zygosity). FC has been found to be very useful for studying mixed cell populations; applications include fetomaternal hemorrhage quantitation, determination of survival/ clearance of transfused RBCs, phenotyping of transfused patients’ RBCs, evaluation of bone marrow transplant engraftment, and study of RBC chimerism and mosaicism.

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Section: Detection and Quantitation Of Globulins Red Blood Cell-boundmentioning

confidence: 92%

Flow Cytofluorometric Analysis in Red Blood Cell Immunology

Arndt¹,

Garratty²

2004

Transfus Med Hemother

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“…It allows the selective quantitation of immunoglobulin subtypes [1]. However, the practical importance of this enhanced precision is controversial [16], because the correlation of disease severity and red blood cell-bound immunoglobulin is limited.…”

Section: Quantitation Of Red Blood Cell-bound Immunoglobulinmentioning

confidence: 99%

Principles and Applications of Red Blood Cell Flow Cytometry

Wagner¹,

Flegel²

1998

Transfus Med Hemother

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Objective: In this review we address principles and important applications of flow cytometry in immunohematology. Sources and Criteria: Some studies of the theoretical back-ground and recent publications of current applications were selected. Results: The direct, indirect and two-color fluorescence as well as the ‘one-antigen-one-antibody’ model are addressed. Possible standardizations and quality controls are discussed. Flow cytometric analyses with erythrocytes bear special problems such as agglutination, which is of lesser im-portance with other cells types. Possible applications include the quantitation of antigen density, antibody concentration, red blood cell bound immunoglobulin, and red blood cell sub-populations. Conclusion: Red blood cell flow cytometry is very sensitive, has a high potential for automatization, and hence may gain importance especially for many routine tasks in the future.

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“…Flow cytometry (FC) has been previously utilized for antibody detection, including the detection of red cell autoantibodies (4,5), HLA alloantibodies (6,7), platelet crossmatching (8)(9)(10), and other immmunohematological applications (11,12). Recently, FC was also introduced for the screening of antibodies to red cells for automation by Roback et al (3,13).…”

Section: Introductionmentioning

confidence: 99%