Correlation between HDL2, HDL3 and serum ferritin levels with fatty liver and NAFLD activity score (NAS) in liver histology of organ donors

Abstract

Background Nonalcoholic fatty liver disease (NAFLD) is one of the most important liver diseases. High-density lipoprotein (HDL) has anti-atherogenic properties and its reduction can be associated with fatty liver. Serum ferritin levels are usually elevated in patients with NAFLD. This study aimed to evaluate the correlation between HDL subtypes and serum ferritin levels with evidence of NAFLD in liver histology of organ donors. Methods One hundred organ donor patients who were eligible for the study were included in the study and ferritin; HDL2 and HDL3 were measured in blood samples. Donated liver tissue biopsy specimens were evaluated for fatty liver and NAFLD activity score (NAS). In addition, AST and ALT were measured in recipients 24 h after transplant. All data abstracted and analyzed statistically. Results Serum HDL2 levels and HDL2/HDL3 ratio in patients with NAS > 1 were significantly lower (P < 0.05). Serum levels of HDL3 and ferritin were not significantly associated with NAS >1 (P > 0.05). In addition, serum ferritin > 1000 ng/ml in organ donors associated with increased AST and ALT levels 24 h after transplantation in the liver organ recipient. Conclusions Lower HDL2 values and HDL2/HDL3 ratio were associated with increased NAFLD activity score, but HDL3 and ferritin did not show such a relationship. In addition, higher levels of ferritin in organ donors may be associated with increased AST and ALT 24 h after liver transplantation in the organ recipient.

High-density lipoproteins and non-alcoholic fatty liver disease

“…In accordance with the notion that both the number as well as the size of HDL particles is reduced in NAFLD, Orozco Morales et al have found that the decrease in total plasma HDL-cholesterol and apoA1 levels in type II diabetics with NAFLD as compared to those without NAFLD was associated with a re-distribution within the HDL subclasses from relatively large HDL2b particles to small HDL3c particles [ 37 ]. A similar shift in the HDL sub-fractions was observed by Nikeghbalian et al in subjects with a relatively high NAFLD activity score as compared to those with a low NAFLD activity score in healthy liver donors [ 38 ]. The NAFLD-associated significant reduction in the HDL2/HDL3 ratio appears to be driven by a decrease in total HDL2 levels in the context of a minor change in total HDL3 levels [ 38 ].…”

“…A similar shift in the HDL sub-fractions was observed by Nikeghbalian et al in subjects with a relatively high NAFLD activity score as compared to those with a low NAFLD activity score in healthy liver donors [ 38 ]. The NAFLD-associated significant reduction in the HDL2/HDL3 ratio appears to be driven by a decrease in total HDL2 levels in the context of a minor change in total HDL3 levels [ 38 ]. The NAFLD-associated decrease in HDL particle size is paralleled by a change in the lipid composition, i.e.…”

Antiferroptosis therapy alleviated the development of atherosclerosis

“…NAS include individual biopsy scores for steatosis (0−3), lobular inflammation (0−3), and hepatocellular ballooning (0−2) (Table S2 ). 46 Serum ox‐LDL of mice was detected by commercial ELISA assay kit (Cat. CSB‐E07933m; CUSBIO, Wuhan, China).…”