Correlation between epitopes on hemagglutinin of measles virus and biological activities: passive protection by monoclonal antibodies is related to their hemagglutination inhibiting activity

Inhibition of measles virus infection and fusion with peptides corresponding to the leucine zipper region of the fusion protein.

“…strain (Buckland et al, 1987). Malvoisin & Wild, 1990) or anti-HA (MAb 55 ; Giraudon & Wild, 1985) for 30 min at 4 mC (ascites fluids diluted 1 : 500). After washing three times in PBS the cells were incubated with a 1 : 100 dilution of fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse Ig (Dako) for 30 min at 4 mC.…”

“…In order to characterize the active region, two overlapping peptides, F455-470 and F468-487, were tested for fusion inhibition. F455-470 failed to inhibit MV-induced fusion in Vero cells at concentrations up to 400 µM, whereas F468-487 inhibited fusion at 400 µM and (Malvoisin & Wild, 1990) or anti-HA MAb 55 (Giraudon & Wild, 1985) for 30 min at 4 mC (ascites fluids diluted 1 : 500). After washing three times in PBS the cells were incubated with a 1 : 100 dilution of FITC-conjugated goat antimouse Ig (Dako) for 30 min at 4 mC.…”

Haemagglutinin of Measles Virus: Purification and Storage with Preservation of Biological and Immunological Properties

“…An apparently normal anti-H antibody response observed after immunization with formalin-inactivated measles virus was found to be unable to protect efficiently against the virus-induced disease (Norrby et al, 1975). By contrast, full passive protection of mice against intracerebrally inoculated measles virus could be observed with several anti-H antibodies that exhibited anti-haemagglutination activity (Giraudon & Wild, 1985). However, one of these anti-H monoclonal antibodies, CL55, although protecting mice against acute measles virus-induced encephalitis, instead induced a delayed encephalitis.…”

“…It has been suggested that important antigenic sites on the glycoproteins are lost during virus inactivation . More recently, a monoclonal antibody recognizing a specific epitope of the H glycoprotein while preventing acute infection was found to induce a delayed encephalitis when administered simultaneously with the intracerebral inoculation of a neuro-adapted measles virus strain in mice (Giraudon & Wild, 1985). The use of purified viral surface components with well-defined biological properties as subunit vaccines should further help in clarifying the role of antigenic epitopes of the purified protein in virus-host interactions.…”

“…To 50 lal of crude soluble [35S]methionine-radiolabelled viral antigen, 3 ~tl of mouse sc, um or monoclonal antibodies directed against H, the nucleoprotein (NP), the membrane protein (M) (kindly provided by P. Giraudon) (Giraudon & Wild, 1985) and F (provided through the courtesy of E. Norrby) were added and incubated in a final volume of 500 ~tl of LB on ice for 2 h. The immune complexes were precipitated by adding Protein A-Sepharose CL-4B (Pharmacia) according to the procedure described by Sheshberadaran et al (1983). After dissociation, the radioactive material precipitated was analysed by SDS-PAGE, followed by gel soaking in Enlightning (New England Nuclear) and autoradiography using Agfa Gevaert Curix RP2 100AFW films with a Dupont Cronex lightning plus screen.…”