Correlation between DNA defect and sperm-head morphology

Cassuto, Nino Guy; Hazout, A.; Hammoud, Ibrahim; Balet, R.; Bouret, Dominique; Barak, Yona; Jellad, Sonia; Plouchart, Jean Marie; Selva, J.; Yazbeck, Chadi

doi:10.1016/j.rbmo.2011.10.006

Cited by 91 publications

(66 citation statements)

References 39 publications

(42 reference statements)

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“…Alteration in sperm chromatin compaction in infertile men, (protamine deficiency or incomplete sulfhydryl oxidation) may contribute to head morphology defects as described in animal models [14,41]. Sperm morphology has been correlated with quality of sperm DNA [6,43], this is in contrast to other studies where no association of sperm morphology and DFI was found [12,49]. Spermatozoa with compromised genomic integrity may fertilize but may not sustain the pregnancy due to damaged DNA.…”

Section: Discussionmentioning

confidence: 90%

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

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Purpose Standard semen parameters are poor predictors of fertility potential. To date, apart from, paternal karyotyping sperm factors are not evaluated in recurrent pregnancy loss (RPL), only recent studies have emphasized the role of sperm factors in early embryonic development as sperm transcribes genes critical for early embryonic development. Sperm DNA integrity is useful diagnostic and prognostic marker and has clinical implications in idiopathic recurrent pregnancy loss (iRPL) following spontaneous conception. The aim of this study was to assess DNA integrity in cases experiencing iRPL following spontaneous conception. Methods Semen samples from 45 patients and 20 controls were analyzed as per WHO 1999 guidelines and sperm chromatin structure assay (SCSA) was used to measure DNA fragmentation index (DFI). Results By applying receiver operating curve (ROC) analysis, sperm DFI of approximately 26 % was found in male partner of couples experiencing iRPL. Conclusions Our data indicate that sperm from men with a history of iRPL have a higher percentage of DNA damage as compared to control group, and this can explain pregnancy loss in these patients. Men with higher DFI are infertile whereas men with lower DFI (26 %) are able to conceive but experience recurrent pregnancy loss. Thus it is important to evaluate sperm DFI in couples experiencing iRPL to understand exact aetiology of RPL and determine prognosis and management.

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Section: Discussionmentioning

confidence: 90%

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

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“…Nonetheless, there are many reports suggesting that sperm vacuoles reflect sperm chromatin packaging/DNA abnormalities [1,4,6,[10][11][12][13][14][15][16].…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that sperm vacuoles should be regarded as normal features of the sperm head that occur naturally during the process of condensation of sperm nuclei, and should not be regarded as degeneration but as physiological changes that do not compromise the sperm quality [5,6]. Meanwhile, studies suggest that the presence of sperm vacuoles is related to male sub fertility [7], lower mitochondrial membrane potential [8], higher incidence of chromosomal abnormalities [8,9], and sperm chromatin packaging/DNA abnormalities [1,4,6,[10][11][12][13][14][15][16]. It has also been suggested that sperm vacuoles reflect non-reacted acrosome [17,18].…”

Section: Introductionmentioning

confidence: 99%

The prevalence of sperm with large nuclear vacuoles is a prognostic tool in the prediction of ICSI success

Setti

Braga

Vingris

et al. 2013

J Assist Reprod Genet

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Purpose To investigate if there is a correlation between the prevalence of sperm with large nuclear vacuoles (LNV) and intracytoplasmic sperm injection (ICSI) outcomes. Methods Two hundred male patients undergoing ICSI had their sperm morphology evaluated through motile sperm organelle morphology examination (MSOME) and the percentage of LNV sperm was recorded and correlated to the ICSI outcomes.Results The percentage of sperm with LNV negatively influenced the blastocyst formation (S: 16.9, R 2 : 20.5 %, p=0.004) and implantation (S: 34.7, R 2 : 26.2 %, p=0.001). There were significant differences in the percentage of sperm with LNV between patients in which pregnancy was achieved or not (22.2 % vs. 28.4 %, p<0.001) and in patients with ongoing pregnancy or not (22.4 % vs. 28.5 %, p<0.001). The incidence of sperm with LNV was determinant to the decreased odds of pregnancy (OR: 0.74, p<0.001) and increased odds of miscarriage (OR: 1.46, p<0.001). The area under the curve (AUC) was sufficient to distinguish between couples which did achieve pregnancy or not (AUC: 0.922, p<0.001).Conclusions The MSOME is a prognostic tool in the prediction of ICSI success and could be used to select patients that should have their sperm selected by MSOME for ICSI.

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“…We can predict that the injection of these compromised spermatozoa may affect the outcome of assisted reproduction in multiple ways, including effects on the fertilization rates, embryo cleavage, and pregnancy rates. In addition, our data show that high level of seminal TGFß1 (>100 ng/ml) which is a critical semen parameter associated with infertility, is a predictive factor for an increased risk of sperm DNA damage [14][15][16][17].…”

Section: Resultsmentioning

confidence: 66%

“…Subjects currently on any medication or antioxidant supplementation were not included [16][17][18][19][20][21][22][23][24]. In addition, subjects with testicular varicocele, genital infection, leukocytespermia, chronic illness and serious systemic diseases, smokers and alcoholic men were excluded from the study because of their well-known high seminal ROS levels and decreased antioxidant activity.…”

Section: Exclusion Criteriamentioning

confidence: 99%

Effect of Seminal Transforming Growth Factorß1 (TGFß1) and Glutathione on Apoptosis in Spermatozoa from Tunisian Infertile Men

Ben Ali

2017

Andrology

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We aimed to compare the effect of TGFB1 and glutathione on sperm necrosis in three groups of patients with increased necrospermia. The study included 120 men aged 37.6 ± 4.6 years consulting for sterility of the couple. Patients were subdivided into three groups according to the percentage of necrotic spermatozoa: necrospermia<30%; N=40), moderate necrozoospermia (50-80%, n=45) and severe necrozoospermia (>80%) For each patient, the sperm parameters were evaluated according to WHO standards, TGFB1 and glutathione Oxidized and reduced was carried out by the ELISA technique and by spectrophotometry respectively. We found a significant increase in the levels of TGFβ1 and DFI in moderate and severe necrospermia groups and positive correlations between these two factors and sperm parameters (numeration, mobility and morphology). In addition, a significant decrease in seminal GHSt was observed in the necrozoospermic groups compared to the control group. A strong correlation was observed between the degree of necrozoospermia and the fragmentation of sperm DNA (r=0.878, p=0.001). In addition, a significant positive correlation between TGFβ1 and DFI in the two groups of patients with moderate necrospermia (r=0.43, p<0.05) and severe necrospermia (r=0.52, p<0.05). This confirms that seminal TGFβ1 is a factor in the fragmentation of spermatozoa DNA. These results suggest that decreased glutathione and increased seminal TGFβ1 are important risk factors that can lead to a succession of morphological and functional alterations of spermatozoa resulting in necrozoospermia. Their perturbation in seminal plasma can lead to mediocre results of medically assisted procreation.

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Correlation between DNA defect and sperm-head morphology

Cited by 91 publications

References 39 publications

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

The prevalence of sperm with large nuclear vacuoles is a prognostic tool in the prediction of ICSI success

Effect of Seminal Transforming Growth Factorß1 (TGFß1) and Glutathione on Apoptosis in Spermatozoa from Tunisian Infertile Men

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