Correlation between diverse cyclic lipopeptides production and regulation of growth and substrate utilization by Bacillus subtilis strains in a particular habitat

Abstract

The two Bacillus subtilis strains (DM-03 and DM-04) were isolated from two extremely different habitats; one from the traditional fermented food and another one from a petroleum contaminated soil sample. These strains produced quantitatively and qualitatively different cyclic lipopeptides isoforms under laboratory culture conditions. MALDI-TOF mass spectral analysis revealed that lipopeptide profile varied according to the producing B. subtilis strains; iturins and surfactins isoforms were pre-dominant cyclic lipopeptides produced by B. subtilis DM-03 and DM-04 strains, respectively. A comparative study showed that these strains possessed distinct preferences for the carbon and nitrogen substrates, temperature and pH for optimal growth and biosurfactant production. Our study documented that the cyclic lipopeptide isoforms produced by the respective strains played an important role in the utilization of available hydrophobic substrate(s) from their natural habitats and conferred some kind of competitive advantage to the producing B. subtilis strains in their parent ecological niche.

Characterization of Bacillus mojavensis KJS-3 for the Promotion of Plant Growth

“…These findings therefore indicate that fengycin, surfactin, and iturin A can be produced by B. mojavensis KJS-3. Cyclic lipopeptides including surfactin, iturin, and fengycin are the major classes of biosurfactants which are known to be produced by Bacillus species [15]. In the LC-MS/MS spectrum, well-resolved groups with peaks between 1,000 and 1060 m/z were observed, and these peaks can be attributed to the isoform ensembles of surfactins, iturins, and fengycins, which are included in the well-known surfactin products produced by…”

The Potentials of Chicken Drops in the Remediation of Petroleum Contaminated Agricultural Soil

“…However, bacterial biosurfactant has been proven effective in enhancing the solubility and biodegradation of petroleum hydrocarbons including Polycyclic aromatic hydrocarbons [14,15]. Microbial production of biosurfactant has been linked to the utilization of available hydrophobic substrates by the producing microbes from their natural habitat, presumably by increasing the surface area of substrates and increasing their apparent solubility [16,17].Therefore the presence of high microbial population in the chicken drops and their subsequent production of biosurfactant may be responsible for the percentage increase polycyclic aromatic degradation of the bioaugmented site as compared to the natural attenuated site ( Figure 2). …”

Surface activity of salt-tolerant Serratia spp. and crude oil biodegradation in saline soil

“…The addition of biosurfactant secreted by microbe can enhance the biodegradation by increasing the bioavailability of the hydrophobic organic pollutants involved crude oil (Mukherjee and Das 2005). Inoculation with biosurfactant producers likely offers the advantage of a continuous supply of surfactant to enhance crude oil degradation in soil.…”