Correlation between cell survival and micronuclei-induction in HeLa cells treated with adriamycin after exposure to various doses of γ-radiation

Jagetia, Ganesh Chandra; Aruna, R

doi:10.1016/s0378-4274(00)00185-5

Cited by 12 publications

(13 citation statements)

References 45 publications

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“…DOX has been reported to induce single and double strand breaks of DNA, micronuclei, chromatid and chromosome aberrations in vitro and in vivo (Bean et al, 1992;Al-Harbi, 1993;Al-Shabanah, 1993;Delvaeye et al, 1993;Jagetia and Nayak, 1996;Jagetia and Aruna, 2000;Jagetia and Nayak, 2000;Dhawan et al, 2003. An identical effect has been observed in the present study, where DOX induced a concentration dependent rise in the MNBNCs in V79 cells exposed to various concentrations of DOX and the dose response was linear quadratic.…”

Section: Discussionsupporting

confidence: 85%

“…A concentration dependent elevation in the cytotoxicity after DOX-treatment has been observed earlier in V79 cells (Suter et al, 1980;Bhuyan et al, 1983;Babudri et al, 1984). Similarly, our earlier studies have shown a concentration dependent decline the survival of HeLa cells exposed to DOX (Jagetia and Nayak, 1996;Jagetia and Aruna, 2000). Likewise, DOX has been also reported to induce dose-dependent cell killing and growth inhibition in various cell lines (Helbig and Speit, 1995;Bogdanović et al, 2004;Gumulec et al, 2014).…”

Section: Discussionsupporting

confidence: 78%

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Bael, Aegle marmelos (L.) Correa, an Indian medicinal plant protects V79 cells against the genotoxic effect of doxorubicin

Jagetia¹,

Venkatesh²

2015

Int. J. Genet. Mol. Biol.

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Alleviation of doxorubicin (DOX)-induced cytotoxicity and genotoxicity by leaf extract of bael also known as Aegle marmelos (AME) was studied in cultured Chinese hamster V79 cells. The optimum protective dose of AME was determined by treating V79 cells with different concentrations of AME before exposure to 10 µg/ml DOX and then by evaluating the cell survival and micronuclei frequency in the cytokinesis blocked V79 cells. MTT assay results revealed that AME pretreatment resulted in a concentration dependent elevation in the cell survival up to 25 μg/ml, whereas a further increase in AME concentration reduced the cell survival. Assessment of DNA damage by micronuclei assay showed that 25 µg/ml AME reduced the micronuclei frequency to a maximum extent. Therefore, 25 μg/ml AME was considered as an optimum chemo-protective concentration and further studies were carried out using this concentration, where V79 cells were treated with 25 μg/ml AME before exposure to different concentrations of DOX. The results of MTT assay at various post-DOX treatment times showed a time and concentration dependent decline in the cell survival with a maximum decline at 72 h post-DOX treatment. The IC 50 values of 122, 108, 88 and 47 µg/ml DOX was observed at 12, 24, 48 and 72 h post-DOX treatment, respectively. Treatment of V79 cells with 25 μg/ml AME before DOX exposure to different concentrations of resulted in a rise in the IC 50 by 60, 24, 44 and 41 µg/ml at 12, 24, 48 and 72 h, respectively. These results were corroborated by clonogenic assay where DOX-treatment caused a concentration dependent decline in the cell survival; whereas treatment of V79 cells with 25 µg/ml AME before DOX exposure arrested the DOX-induced decline in the cell survival. The micronuclei frequency increased in a concentration dependent manner in cells exposed to DOX, whereas AME pretreatment significantly reduced the DOX-induced micronuclei formation. Our results suggest that AME did reduce the cytotoxic and genotoxic effects of DOX and may be useful in clinical setup to reduce DOX-induced toxicity.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 78%

Bael, Aegle marmelos (L.) Correa, an Indian medicinal plant protects V79 cells against the genotoxic effect of doxorubicin

Jagetia¹,

Venkatesh²

2015

Int. J. Genet. Mol. Biol.

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“…Decreases in the ratio of PCE to mature erythrocytes are considered an indicator of mutagen-induced bone marrow cytotoxicity (Criswell et al, 1998). It has been reported that the DNA double strand breaks may subsequently be converted to chromosome breaks that may finally be expressed as micronuclei after one cell division (Jagetia and Aruna, 2000). The increased frequency of CA and micronuclei in ADR administered animals observed in this study might be due to the increased DNA strand breaks.…”

Section: Groupsmentioning

confidence: 49%

Salubrious effects of lipoic acid against adriamycin-induced clastogenesis and apoptosis in Wistar rat bone marrow cells

et al. 2006

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“…, betulinic acid, alphitolic acid, ursolic acid, ester derivatives of betulinic acid such as 7β-(4-hydroxy-benzoyloxy)-betulinic acid, 7β-(4-hydro-3-methoxy-benzoyloxy)-betulinic acid and 27-(4-hydroxy-3-methoxy-benzoyloxy)-betulinic acid] (Oliveira et al , 2003; Schuhly et al , 1999) and saponins (Alviano et al , 2008). DXR has been reported to induce micronuclei, chromatid and chromosomal aberrations, and DNA single- and double-strand breaks in vitro and in vivo (Bean et al , 1992; Al-Harbi, 1993; Al-Shabanah, 1993; Delvaeye et al , 1993; Jagetia and Nayak, 1996, 2000; Shan et al , 1996; Dhawan et al , 2003; Jagetia and Aruna, 2000). In addition, the major acute toxicity induced by DXR is bone marrow suppression, while the long-term clinical usefulness is limited by a cumulative, dose-dependent, irreversible, chronic cardiotoxicity that manifests itself as congestive heart failure or cardiomyopathy (Van Acker et al , 1995, 2000).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay

et al. 2014

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The aim of this study was to evaluate the mutagenicity (clastogenicity/aneugenicity) of a glycolic extract of Ziziphus joazeiro bark (GEZJ) by the micronucleus assay in mice bone marrow. Antimutagenic activity was also assessed using treatments associated with GEZJ and doxorubicin (DXR). Mice were evaluated 24–48 h after exposure to positive (N-nitroso-N-ethylurea, NEU - 50 mg.kg−1 and DXR - 5 mg.kg−1) and negative (150 mM NaCl) controls, as well as treatment with GEZJ (0.5–2 g.kg−1), GEZJ (2 g.kg−1) + NEU and GEZJ (2 g.kg−1) + DXR. There were no significant differences in the frequencies of micronucleated polychromatic erythrocytes in mice treated with GEJZ and GEJZ + DXR compared to the negative controls, indicating that GEZJ was not mutagenic. Analysis of the polychromatic:normochromatic erythrocyte ratio revealed significant differences in the responses to doses of 0.5 g.kg−1 and 1–2 g.kg−1 and the positive control (NEU). These results indicated no systemic toxicity and moderate toxicity at lower and higher doses of GEZJ. The lack of mutagenicity and systemic toxicity in the antimutagenic assays, especially for treatment with GEZJ + DXR, suggested that phytochemical compounds in Z. joazeiro bark attenuated DXR-induced mutagenicity and the moderate systemic toxicity of a high dose of Z. joazeiro bark (2 g.kg−1). Further studies on the genotoxicity of Z. joazeiro extracts are necessary to establish the possible health risk in humans and to determine the potential as a chemopreventive agent for therapeutic use.

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Correlation between cell survival and micronuclei-induction in HeLa cells treated with adriamycin after exposure to various doses of γ-radiation

Cited by 12 publications

References 45 publications

Bael, Aegle marmelos (L.) Correa, an Indian medicinal plant protects V79 cells against the genotoxic effect of doxorubicin

Bael, Aegle marmelos (L.) Correa, an Indian medicinal plant protects V79 cells against the genotoxic effect of doxorubicin

Salubrious effects of lipoic acid against adriamycin-induced clastogenesis and apoptosis in Wistar rat bone marrow cells

Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay

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