Correlation between cell attachment areas after 2h of culture and osteogenic differentiation activity of rat mesenchymal stem cells on hydroxyapatite substrates with various surface properties

Cheng, Kan; Hirose, Motohiro; Wang, Xiupeng; Sogo, Yu; Yamazaki, Atsushi; Ito, Atsuo

doi:10.1016/j.bbrc.2012.11.054

Cited by 5 publications

(1 citation statement)

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“…As an osteoconductive ceramic which resembles the inorganic component of bone, hydroxyapatite (HA; Ca 10 (PO 4 ) 6 (OH) 2 ) is interesting to biologists and biomaterial scientists alike. To date, HA has found use in applications such as a bone filler (Frame et al, 1981), orthopedic implant coating (Liu et al, 2009), composite filler (Liu et al, 2013), and cell culture substrate (Cheng et al, 2013). In many of these cases, the ability to control or manipulate the surface properties of HA is very important and informs how successful the application will be.…”

Section: Introductionmentioning

confidence: 99%

An Alternative Approach to the Surface Methacrylation of Non-stoichiometric Hydroxyapatite Nanoparticles for Use in Bone-Inspired Composites

Comeau

Willett

2019

Front. Mater.

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Modification of the inorganic particle surface is one approach toward enhancing the mechanical and swelling properties of a bone-inspired composite. In this study we sought to manipulate the surface of non-stoichiometric nHA particles by utilizing a gentler methacrylation approach with two different methacrylates-methacrylic anhydride (MAh) and glycidyl methacrylate (GMA). While silanization of nHA (Si-nHA) is a recognized functionalization approach, one notable disadvantage is the need for relatively harsh reaction conditions. Here, ATR-FTIR and liquid state 13 C-NMR provided evidence for a stronger affinity of the nHA surface for methacrylic anhydride compared to glycidyl methacrylate under the same reaction conditions; fewer GMA molecules reacted with the nHA surface. However, the affinity of MAh is more electrostatic in nature, whereas GMA attachment involves some covalent bond formation. In addition, while in water, there was no detectable dependence of particle settling on methacrylate choice, in ethanol, native nHA and nHA methacrylated with GMA each had detectably greater stability in suspension than the other sample groups. Lastly, the addition of GMA-nHA detectably increased the dynamic stiffness and did not impact the swelling in 37 • C water of a GelMA-based composite compared to that observed upon adding native nHA. Overall, the GMA-based methacrylation approach was found to be a viable alternative to silanization and offers possibilities for future fabrication of bone-inspired composites.

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