Correlation between biofilm formation and production of polysaccharide intercellular adhesin in clinical isolates of coagulase-negative staphylococci

Chokr, Ali; Watier, Denis; Eleaume, Heïdy; Pangon, B.; Ghnassia, J. C.; Mack, Dietrich; Jabbouri, Saı̈d

doi:10.1016/j.ijmm.2006.02.018

Cited by 77 publications

(75 citation statements)

References 33 publications

(24 reference statements)

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“…Chromosomal DNA was extracted using a Genomic DNA prep Plus kit (A&A Biotechnology). PCR was applied to detect the presence of icaADBC (Chokr et al, 2006).…”

Section: Methodsmentioning

confidence: 99%

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Clonality, virulence and the occurrence of genes encoding antibiotic resistance among Staphylococcus warneri isolates from bloodstream infections

Szczuka

Krzymińska

Kaznowski

2016

Journal of Medical Microbiology

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Coagulase-negative Staphylococcus warneri is an opportunistic pathogen capable of causing several infections, especially in patients with indwelling medical devices. We evaluated the virulence-associated properties of 23 clinical isolates recovered from blood specimens. In addition, the carriage of biofilm-associated genes, as well as antibiotic-resistant genes, was identified. S. warneri isolates appeared to be clonally unrelated and revealed a high degree of genetic diversity. All isolates revealed adhesion to epithelial cells, and 43.5 % of strains invaded the cells. Moreover, 52 % of isolates formed biofilm in vitro. PCR analysis demonstrated the presence of the ica operon, in two of the 12 biofilm-positive isolates. This indicated that biofilm formation, in this species, is not restricted to strains harbouring icaADBC genes, encoding polysaccharide intercellular adhesion. Analysis by confocal laser scanning microscopy revealed that biofilm-forming strains formed a three-dimensional structure, composed of mainly living cells. All strains revealed cell-contact cytotoxicity that was strongly associated with biofilm formation. Moreover, cell-free supernatants, of 95 % of the isolates, expressed a cytotoxic activity which caused the destruction of HeLa cells. S. warneri capable of forming biofilm carried significantly more genes encoding resistance to beta-lactams, aminoglicosides and macrolide-lincosamide streptogramin B antibiotics than biofilm-negative isolates. We have shown that tigecycline/ rifampicin is effective against bacteria growing as a biofilm. The biofilm inhibitory concentration range of tigecycline/rifampicin was 1 µg ml À1 . Results indicated that S. warneri have the ability to adhere, form biofilm, invade and destroy epithelial cells, which could be important mechanisms contributing to the development of diseases.

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“…Chromosomal DNA was extracted using a Genomic DNA prep Plus kit (A&A Biotechnology). PCR was applied to detect the presence of icaADBC (Chokr et al, 2006).…”

Section: Methodsmentioning

confidence: 99%

“…It should be emphasised that most S. warneri strains form biofilm via ica-independent mechanisms. Previously, Chokr et al (2006) reported that four S. warneri strains carried icaADBC genes in their genomes. However, only one of those strains had the ability to produce biofilm in vitro.…”

Section: Biofilm Formation and In Vitro Activity Of Rifampicin Combinmentioning

confidence: 99%

Clonality, virulence and the occurrence of genes encoding antibiotic resistance among Staphylococcus warneri isolates from bloodstream infections

Szczuka

Krzymińska

Kaznowski

2016

Journal of Medical Microbiology

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“…A biofilm is defined as a microbially derived sessile community surrounded by a self-producing extracellular polymer matrix. The biofilm matrix, a homoglycan composed of β-1,6-linked N-acetylglucosamine residues, is involved in intercellular adhesion and is referred to as polysaccharide intercellular adhesion (PIA) [23]. Biofilm formation includes several sequential steps in which planktonic bacteria initially attach to a solid surface, that may be either unmodified or coated with host plasma proteins, followed by cell proliferation, cell-cell interaction, and production of an extracellular polymeric matrix, where bacteria accumulate in multilayered clusters (Figure 2).…”

Section: Pdt For Inactivate Biofilm Formationmentioning

confidence: 99%

Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?

Bloise¹,

Minzioni²,

Imbriani³

et al. 2017

Photomedicine - Advances in Clinical Practice

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Recent developments in light-controlled therapies (e.g., photodynamic and photothermal therapies) provide promising strategies to prevent and suppress bacterial infections, which are a leading cause of morbidity and mortality. Antibacterial photodynamic therapy (aPDT) has drawn increasing attention from the scientific society for its potential to kill multidrug-resistant pathogenic bacteria and for its low tendency to induce drug resistance. In this chapter, we summarize the mechanism of action of aPDT, the photosensitizers, as well the current developments in terms of treating Gram-positive and Gram-negative bacteria. The chapter also describes the recent progress relating to photomedicine for preventing bacterial infections and biofilm formation. We focus on the laser device used in aPDT and on the light-treatment parameters that may have a strong impact on the results of aPDT experiments. In the last part of this chapter, we survey on the various nanoparticles delivering photoactive molecules, and photoactive-nanoparticles that can potentially enhance the antimicrobial action of aPDT.

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“…Finally, the absorbance was measured with a spectrophotometer at 570 nm. This quantity is directly proportional to the number of cells with an intact membrane Ali Chokr has identified and characterized the properties of these strains and deposited it to be enclosed within the collection of microorganisms of Pasteur Institute in 2007 [19][20][21] . The other strains were ATCC that were stored in glycerol stocks at -80°C and used as required.…”

Section: Evaluation Of the Anti-proliferative Activitymentioning

confidence: 99%

Antibacterial, Antioxidant and Antiproliferative Activities of the Hydroalcoholic Extract of the Lebanese Annona Squamosa L. Seeds

Nasser¹,

Majid²,

Falah³

et al. 2017

Int. Res. J. Pharm.

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To determine the chemical composition of the seeds of the Lebanese Annona squamosa L., a phytochemical screening was performed on a water/methanol (v/v) extract. Following that, several quantitative tests were carried out to determine the content of the seeds in the secondary metabolites. Also, the nutritive and medical importance was evaluated in order to realize some biological activities such as antibacterial, antioxidant and anti-proliferative activities. The obtained results showed that the hydroalcoholic extract from the seeds of this fruit contains a variety of primary and secondary metabolites like alkaloids, resins, saponin, phenols, terpenoids, flavonoids, carbohydrates and quinones. In addition, it has a low antioxidant activity and a bacteriostatic activity that is greater than a bactericidal agent. Finally, the seeds' extract presented an anti-proliferative activity that is more effective on HCT-116 colon cells than on HT-29 epithelial cells. Further research studies need to be conducted to confirm the antibacterial, antioxidant and anti-proliferative effects of the seeds found in this fruit.

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Correlation between biofilm formation and production of polysaccharide intercellular adhesin in clinical isolates of coagulase-negative staphylococci

Cited by 77 publications

References 33 publications

Clonality, virulence and the occurrence of genes encoding antibiotic resistance among Staphylococcus warneri isolates from bloodstream infections

Clonality, virulence and the occurrence of genes encoding antibiotic resistance among Staphylococcus warneri isolates from bloodstream infections

Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?

Antibacterial, Antioxidant and Antiproliferative Activities of the Hydroalcoholic Extract of the Lebanese Annona Squamosa L. Seeds

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