Correlation between bacterial biofilms and osteitis in patients with chronic rhinosinusitis

Dong, Dong; Zhao, Yue; Zhang, Hongyan; Liu, Jia; Xie, Yan; Wang, Jia

doi:10.1002/lary.24424

Cited by 24 publications

(21 citation statements)

References 32 publications

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“…Similarly, Kaya et al (2013) also detected poly-microbial biofilms of S. aureus and PA in the middle ear (Saunders et al, 2011; Kaya et al, 2013). In CRS patients, multi-species biofilms have been associated with increased mucosal inflammation, more severe osteitis, higher incidence of recurrent infection (Li et al, 2011; Dong et al, 2014) and postoperative outcome (Singhal et al, 2011), and post-surgery progressions (Bendouah et al, 2006). …”

Section: Discussionmentioning

confidence: 99%

“…S. aureus and PA have been isolated from upper respiratory tract infections including several chronic diseases such as chronic otitis media, cholesteatoma, chronic adenoiditis, chronic sinusitis, post-operative trampansomay, and nasal polyposis (Post et al, 2004; Bendouah et al, 2006; Boase et al, 2013). In chronic rhinosinusitis (CRS) patients, multi-species biofilms have been associated with enhanced mucosal inflammation, more severe osteitis, higher incidence of recurrent infection (Li et al, 2011; Dong et al, 2014), and postoperative outcomes (Singhal et al, 2011), and post-surgery progression (Bendouah et al, 2006). Furthermore, S. aureus and PA have been isolated from multi-species biofilms that are frequently found on indwelling medical devices such as prostheses, stents, implants, catheters, and endotracheal tube (Percival et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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In vitro Multi-Species Biofilms of Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa and Their Host Interaction during In vivo Colonization of an Otitis Media Rat Model

Yadav

Chae

et al. 2017

Front. Cell. Infect. Microbiol.

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Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) are known to cause biofilm-related infections. MRSA and PA have been frequently isolated from chronically infected wounds, cystic fibrosis, chronic suppurative otitis media (CSOM), and from indwelling medical devices, and these bacteria co-exist; however, their interaction with each-other or with the host is not well known. In this study, we investigated MRSA and PA multi-species biofilm communities in vitro and their interaction with the host during in vivo colonization using an OM rat-model. In-vitro biofilm formation and in-vivo colonization were studied using CV-microtiter plate assay and OM rat-model respectively. The biofilms were viewed under scanning electron microscope and bacteria were enumerated using cfu counts. The differential gene expressions of rat mucosa colonized with single or multi-species of MRSA or PA were studied using RNA-sequencing of total transcriptome. In multi-species in-vitro biofilms PA partially inhibited SA growth. However, no significant inhibition of MRSA was detected during in-vivo colonization of multi-species in rat bullae. A total of 1,797 genes were significantly (p < 0.05) differentially expressed in MRSA or PA or MRSA + PA colonized rat middle ear mucosa with respect to the control. The poly-microbial colonization of MRSA and PA induced the differential expression of a significant number of genes that are involved in immune response, inflammation, signaling, development, and defense; these were not expressed with single species colonization by either MRSA or PA. Genes involved in defense, immune response, inflammatory response, and developmental process were exclusively up-regulated, and genes that are involved in nervous system signaling, development and transmission, regulation of cell growth and development, anatomical and system development, and cell differentiation were down-regulated after multi-species inoculation. These results indicate that poly-microbial colonization induces a host response that is different from that induced by single species infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro Multi-Species Biofilms of Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa and Their Host Interaction during In vivo Colonization of an Otitis Media Rat Model

Yadav

Chae

et al. 2017

Front. Cell. Infect. Microbiol.

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“…Bacterial microcolonies were found in 1 CRSsNP and 2 CRSwNP cases, as well as in 2 of 6 control cases. 405 Dong et al 406 demonstrated that 85% of CRS patients with mucosa with bacterial biofilms had some form of osteitis present. Additionally, the histopathologic grade, GOSS score, and HU value in patients with bacterial biofilms were greater than those without biofilms.…”

Section: S55mentioning

confidence: 99%

International Consensus Statement on Allergy and Rhinology: Rhinosinusitis

Orlandi

Kingdom

Hwang

et al. 2016

Int Forum Allergy Rhinol

540

867

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Background:The body of knowledge regarding rhinosinusitis (RS) continues to expand, with rapid growth in number of publications, yet substantial variability in the quality of those presentations. In an effort to both consolidate and critically appraise this information, rhinologic experts from around the world have produced the International Consensus Statement on Allergy and Rhinology: Rhinosinusitis (ICAR:RS). Methods:Evidence-based reviews with recommendations (EBRRs) were developed for scores of topics, using previously reported methodology. Where existing evidence was insufficient for an EBRR, an evidence-based review (EBR) was produced. The sections were then synthesized and the entire manuscript was then reviewed by all authors for consensus. Results:The resulting ICAR:RS document addresses multiple topics in RS, including acute RS (ARS), chronic RS (CRS) with and without nasal polyps (CRSwNP and CRSsNP), recurrent acute RS (RARS), acute exacerbation of CRS (AE-CRS), and pediatric RS. Conclusion:As a critical review of the RS literature, ICAR:RS provides a thorough review of pathophysiology and evidence-based recommendations for medical and surgical treatment. It also demonstrates the significant gaps in our understanding of the pathophysiology and optimal management of RS. Too o en the foundation upon which these recommendations are based is comprised of lowerlevel evidence. It is our hope that this summary of the evidence in RS will point out where additional research efforts may be directed. C 2016 ARS-AAOA, LLC. Key Words:rhinosinusitis; chronic rhinosinusitis; acute rhinosinusitis; recurrent acute rhinosinusitis; evidence-based medicine; systematic review; endoscopic sinus surgery List of Abbreviations Used

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“…Despite the frequent co‐occurrence of bacteria and CRS, the role of bacteria in promoting neo‐osteogenesis is uncertain . Neo‐osteogenesis has been correlated with mucosal biofilms in CRS, and biofilm‐forming pathogens such as coagulase‐negative Staphylococcus aureus , Staphylococcus aureus , and Pseudomonas aeruginosa are among the most common organisms cultured from CRS patients . However, no evidence has demonstrated that bacterial organisms exist in neo‐osteogenic bone in either humans or animal models of CRS.…”

mentioning

confidence: 99%

Clinical predictors of neo‐osteogenesis in patients with chronic rhinosinusitis

Huang

Hajjij

et al. 2015

Int Forum Allergy Rhinol

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Correlation between bacterial biofilms and osteitis in patients with chronic rhinosinusitis

Abstract: A total of 84.8% of the bone underlying mucosa with BBF had some form of osteitis in ethmoid sinus, and approximately 46.4% of CRS patients were from a subgroup with both BBF and osteitis. The volume of BBF correlated well with severity of osteitis in CRS patients.

Cited by 24 publications

References 32 publications

In vitro Multi-Species Biofilms of Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa and Their Host Interaction during In vivo Colonization of an Otitis Media Rat Model

In vitro Multi-Species Biofilms of Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa and Their Host Interaction during In vivo Colonization of an Otitis Media Rat Model

International Consensus Statement on Allergy and Rhinology: Rhinosinusitis

Clinical predictors of neo‐osteogenesis in patients with chronic rhinosinusitis

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