Correlation between aneuploidy, standard morphology evaluation and morphokinetic development in 1730 biopsied blastocysts: a consecutive case series study

Minasi, Maria Giulia; Colasante, A.; Riccio, Teresa; Ruberti, Alessandra; Casciani, Valentina; Scarselli, Filomena; Spinella, Francesca; Fiorentino, Francesco; Varricchio, Maria Teresa; Greco, Ermanno

doi:10.1093/humrep/dew183

Cited by 284 publications

(228 citation statements)

References 59 publications

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“…Curiously, t2 is shorter when embryo was incubated in single culture medium than sequential culture medium (27.36 ± 4.12 vs. 29.09 ± 4.86 h) [7]. The mean time of t2 is no significant between euploid (28 h) and aneuploid embryos (28.4 h) [2]. The development of the 2-cell stage may be faster in implanted embryos but no significant in chromosome status.…”

Section: Morphokinetic Markersmentioning

confidence: 95%

“…In euploid embryos, the mean time of tPNa is 10.2 h and 10.1 h in aneuploid embryos [2]. Therefore, the timing of pronuclear appearance has no significant effect on implantation potential and chromosome status.…”

Section: Morphokinetic Markersmentioning

confidence: 96%

“…Azzarello et al [4] claimed that the timing of tPNf was longer in live birth group (24.9 ± 0.6 vs. 23.3 ± 0.4 h), and there was no live birth if the timing of PN breakdown was less than 20 h. The timing of PN breakdown was equal between implanted and non-implanted embryos [3,5]. The mean time of tPNf is 24.4 h in euploid embryos and 24.8 h in aneuploid embryos [2]. The timing of pronuclear fading has no significant difference in embryo implantation and chromosome status but no live birth when tPNf is less than 20 h. The milestones of embryo development.…”

Section: Morphokinetic Markersmentioning

confidence: 99%

“…The mean time of tPB2 is 3.9 h in euploid and 4.0 h in aneuploid embryos, respectively. The chromosome integrity of embryos is irrelevant to the timing of second body extrusion [2].…”

Section: Morphokinetic Markersmentioning

confidence: 99%

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Timing of Embryo Cleavage

Lee¹

2017

Embryo Cleavage

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Time-lapse system can provide a culture environment to observe the development of embryos continuously. There are many morphokinetic markers to help us to find out the best quality of embryos. We review the studies to clarify the relationship of markers between implantation potential and embryo chromosome status. Surprisingly, most of markers are controversial or no significant effect on implantation potential and pregnancy rate. We suppose that some uncertain factors may influence embryonic implantation and pregnancy. Here we provide a new method for selecting optimal quality of embryos by many morphokinetic markers in the time-lapse system. Therefore, we can expect that the time-lapse system helps us to choose the good quality embryos for subsequent embryos transfer to improve implantation potential, euploid chromosome and pregnancy rate. Furthermore, studies need to understand the other maternal physical conditions correlation with embryos implantation.

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Section: Morphokinetic Markersmentioning

confidence: 95%

Section: Morphokinetic Markersmentioning

confidence: 96%

Section: Morphokinetic Markersmentioning

confidence: 99%

“…The mean time of tPB2 is 3.9 h in euploid and 4.0 h in aneuploid embryos, respectively. The chromosome integrity of embryos is irrelevant to the timing of second body extrusion [2].…”

Section: Morphokinetic Markersmentioning

confidence: 99%

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Timing of Embryo Cleavage

Lee¹

2017

Embryo Cleavage

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“…They reported no signiicant diference among euploid and aneuploid embryos regarding time-lapse morphokinetics and concluded that morphokinetic parameters can be used in combination with, not instead of, PGS for detecting embryo ploidy status [50]. Conversely, in a retrospective cohort study, a total of 460 embryos were cultured in TLS and biopsied on Day 3.…”

Section: Diferent Studies Based On Time-lapse Observationmentioning

confidence: 99%

Embryo Morphokinetics Based on Time-Lapse Observation

Tabibnejad¹

2017

Embryo Cleavage

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Embryo incubation and evaluation are critical steps in assisted reproductive technology (ART). Conventionally, embryo assessment has been done by embryologists through removing embryos from a conventional incubator during the culture period. Over recent years, time-lapse systems (TLS) have been established which can take digital images of embryos at key points and time intervals. This technique allows embryologists to assess the embryo quality in the steady culture environment. According to TLS studies and prepared algorithm models, it seems that TLS alone or in combination with conventional morphology can be considered as a useful diagnostic tool to determine high-quality embryos and improve embryonic implantation and pregnancy rates. In addition, there were remarkable diferences between embryo developmental time points and intervals regarding embryo gender, embryo fragmentation, and type of ovarian stimulation protocol. For conident conclusion, time-lapse imaging should be evaluated in further studies, and the system should be evaluated for cost/beneit ratio efectiveness in individual laboratory.Keywords: embryo cleavage, embryo morphokinetics, embryoscope, time-lapse imaging IntroductionAssisted reproductive technology (ART) may help infertile couples to realize their dream to have a child in their family, but pregnancy and live birth rates following in vitro fertilization (IVF) still remain low. It is ideal to identify viable embryos with the highest implantation potential to raise IVF success rates. In the traditional IVF practice, embryo assessments are mainly based on the morphologic observation and grade of embryologists at each stage of oocyte and embryonic development. Some features including oocyte and embryo quality, blastomere numbers and regularity, the percentage of fragmentation, and cytoplasmic granularity have been deined as prognostic indicators of successful pregnancy. This traditional embryo assessment method may have some detrimental efects on embryo growth because frequently opening and closing of incubators often cause to the change of embryo culture environmental steadiness. In order to reduce the inter-and intra-observer diference change, the time-lapse imaging (TLI) has been introduced into in vitro fertilization (IVF) laboratory. The application of time-lapse technology to the clinical IVF laboratory has supported more detailed observations on the embryo development researches quickly.The aim of this chapter is to determine whether TLI is useful for selection of "top quality" embryos for transfer to improve ART outcome rather than conventional morphological evaluation. The possible correlation between embryos' sex, embryo fragmentation, treatment protocols, diferent culture media, and embryo morphokinetics will be examined based on some new researches of TLI facilities. Furthermore, various algorithms and predictive models designed in ART cycles with TLI will be discussed.

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