2014
DOI: 10.1016/j.rbmo.2013.12.001
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Correlation between aneuploidy, apoptotic markers and DNA fragmentation in spermatozoa from normozoospermic patients

Abstract: Genetic and biochemical sperm integrity is essential to ensure the reproductive competence. However, spermatogenesis involves physiological changes that could endanger sperm integrity. DNA protamination and apoptosis have been studied extensively. Furthermore, elevated rates of aneuploidy and DNA injury correlate with reproductive failures. Consequently, this study applied the conventional spermiogram method in combination with molecular tests to assess genetic integrity in ejaculate from normozoospermic patie… Show more

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Cited by 35 publications
(38 citation statements)
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“…Agents that induce oxidative stress in spermatozoa cause accumulation of GSSG because the capacity of NADP-dependent GSSG-reducates becomes rate-limiting and the NADPH/NADP ratio decreases. Indeed our results showed that in moderate and severe necrospermia, the rate oxidized glutathione (GSSG) was elevated compared to the control group, and the difference was statistically significant [23][24][25]55]. This confirms the fact that the oxidized form of glutathione is in favored the production of reactive species of oxygen as well as the TGFß1.…”
Section: Discussionsupporting
confidence: 76%
See 1 more Smart Citation
“…Agents that induce oxidative stress in spermatozoa cause accumulation of GSSG because the capacity of NADP-dependent GSSG-reducates becomes rate-limiting and the NADPH/NADP ratio decreases. Indeed our results showed that in moderate and severe necrospermia, the rate oxidized glutathione (GSSG) was elevated compared to the control group, and the difference was statistically significant [23][24][25]55]. This confirms the fact that the oxidized form of glutathione is in favored the production of reactive species of oxygen as well as the TGFß1.…”
Section: Discussionsupporting
confidence: 76%
“…Slides were observed under a microscope (Zeiss, Oberkochen, Germany) equipped with a 100-magnification lens. [54][55][56][57][58][59][60] Spermatozoa with fragmented DNA had browncolored nuclei, whereas the other cells were blue-gray (counter coloration with Harris's haematoxylin) [7,[61][62][63]. On each slide, approximately 500 cells were counted, and the DNA fragmentation index (DFI) was calculated ( Figure 2).…”
Section: Measurement Of Dna Fragmentationmentioning
confidence: 99%
“…Particularly, it has been largely reported a negative correlation between elevated DNA fragmentation index (DFI) and assisted reproduction outcome [7,8]. Although the clinical relevance of sperm DNA fragmentation related to the ART outcome is well known, the causes of sperm DNA damage are still poorly understood [9].…”
Section: Introductionmentioning
confidence: 99%
“…The use of MACS in relation to aneuploidy has also been considered. In ejaculates from normozoospermic patients with implantation failure, aneuploid, apoptotic and DNA-injured spermatozoa decreased significantly after MACS [110]. However, the interactions between apoptotic markers, DNA integrity and aneuploidy and the effect of MACS on these parameters remain unknown [110].…”
Section: In Vitro Studies On the Quality Of Sperm Selected By Macsmentioning
confidence: 99%
“…In ejaculates from normozoospermic patients with implantation failure, aneuploid, apoptotic and DNA-injured spermatozoa decreased significantly after MACS [110]. However, the interactions between apoptotic markers, DNA integrity and aneuploidy and the effect of MACS on these parameters remain unknown [110]. Other study was conducted in order to determine the fertilization potential of selected sperm (n=35) by hamster oocyte penetration and hamster oocyte-intracytoplasmic sperm injection [111].…”
Section: In Vitro Studies On the Quality Of Sperm Selected By Macsmentioning
confidence: 99%