Correlation between aneuploidy, apoptotic markers and DNA fragmentation in spermatozoa from normozoospermic patients

Vendrell, Xavier; Ferrer, Minerva; García-Mengual, Elena; Muñoz, Patricia Martín; Triviño, Juan Carlos; Calatayud, Carmen Ródenas; Rawe, Vanesa Y.; Ruiz-Jorro, Miguel

doi:10.1016/j.rbmo.2013.12.001

Cited by 35 publications

(38 citation statements)

References 81 publications

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“…Agents that induce oxidative stress in spermatozoa cause accumulation of GSSG because the capacity of NADP-dependent GSSG-reducates becomes rate-limiting and the NADPH/NADP ratio decreases. Indeed our results showed that in moderate and severe necrospermia, the rate oxidized glutathione (GSSG) was elevated compared to the control group, and the difference was statistically significant [23][24][25]55]. This confirms the fact that the oxidized form of glutathione is in favored the production of reactive species of oxygen as well as the TGFß1.…”

Section: Discussionsupporting

confidence: 76%

“…Slides were observed under a microscope (Zeiss, Oberkochen, Germany) equipped with a 100-magnification lens. [54][55][56][57][58][59][60] Spermatozoa with fragmented DNA had browncolored nuclei, whereas the other cells were blue-gray (counter coloration with Harris's haematoxylin) [7,[61][62][63]. On each slide, approximately 500 cells were counted, and the DNA fragmentation index (DFI) was calculated ( Figure 2).…”

Section: Measurement Of Dna Fragmentationmentioning

confidence: 99%

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Effect of Seminal Transforming Growth Factorß1 (TGFß1) and Glutathione on Apoptosis in Spermatozoa from Tunisian Infertile Men

Ben Ali

2017

Andrology

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We aimed to compare the effect of TGFB1 and glutathione on sperm necrosis in three groups of patients with increased necrospermia. The study included 120 men aged 37.6 ± 4.6 years consulting for sterility of the couple. Patients were subdivided into three groups according to the percentage of necrotic spermatozoa: necrospermia<30%; N=40), moderate necrozoospermia (50-80%, n=45) and severe necrozoospermia (>80%) For each patient, the sperm parameters were evaluated according to WHO standards, TGFB1 and glutathione Oxidized and reduced was carried out by the ELISA technique and by spectrophotometry respectively. We found a significant increase in the levels of TGFβ1 and DFI in moderate and severe necrospermia groups and positive correlations between these two factors and sperm parameters (numeration, mobility and morphology). In addition, a significant decrease in seminal GHSt was observed in the necrozoospermic groups compared to the control group. A strong correlation was observed between the degree of necrozoospermia and the fragmentation of sperm DNA (r=0.878, p=0.001). In addition, a significant positive correlation between TGFβ1 and DFI in the two groups of patients with moderate necrospermia (r=0.43, p<0.05) and severe necrospermia (r=0.52, p<0.05). This confirms that seminal TGFβ1 is a factor in the fragmentation of spermatozoa DNA. These results suggest that decreased glutathione and increased seminal TGFβ1 are important risk factors that can lead to a succession of morphological and functional alterations of spermatozoa resulting in necrozoospermia. Their perturbation in seminal plasma can lead to mediocre results of medically assisted procreation.

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Section: Discussionsupporting

confidence: 76%

Section: Measurement Of Dna Fragmentationmentioning

confidence: 99%

Effect of Seminal Transforming Growth Factorß1 (TGFß1) and Glutathione on Apoptosis in Spermatozoa from Tunisian Infertile Men

Ben Ali

2017

Andrology

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“…Particularly, it has been largely reported a negative correlation between elevated DNA fragmentation index (DFI) and assisted reproduction outcome [7,8]. Although the clinical relevance of sperm DNA fragmentation related to the ART outcome is well known, the causes of sperm DNA damage are still poorly understood [9].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Sperm DNA Fragmentation and Aneuploidy in 109 Infertile Patients: Are the Two Parameters Correlated?

Santo¹,

Tarozzi²

2016

Gynecol Obstet Case Rep

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Purpose: To evaluate the association between sperm DNA integrity and chromosomes aneuploidies in a considerable population of infertile patients undergoing assisted reproductive treatment (109 male) characterized by both normal and abnormal semen parameters. Methods:In 109 infertile patients with normal and abnormal semen parameters, the assessments of sperm DNA fragmentation by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) and chromosome aneuploidy by fluorescence in situ hybridization (FISH) have been performed on the same semen sample. Sperm samples were collected by masturbation into sterile cups after 3-5 days of sexual abstinence, allowed to liquefy for 30 min at room temperature and then divided in two aliquots, one processed by double gradient centrifugation (DGC) and used for TUNEL assay, whereas the remained aliquot was left unprocessed and used for FISH test. Results:The main results indicate a significant positive correlation between sperm chromosome aneuploidies and DNA integrity in patients with abnormal semen parameters (R=0.516 p=0,000) and negative not significant correlation in patients with normal seminal parameters (R=-0.105, p=0.476). Conclusions:Assuming the positive correlation between the two biomarkers in patients with defective spermatogenesis, high DNA fragmentation index (DFI) could perhaps be indicator of high chromosome aneuploidies level, thus increasing the risk of generating aneuploid embryos. Therefore, a clinical strategy could probably be to integrate also the preimplantation genetic screening (PGS) in ART treatment plan, in addition to the evaluation of sperm DNA fragmentation and/or sperm chromosome aneuploidy, to avoid transfer of chromosomally abnormal embryos.

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“…The use of MACS in relation to aneuploidy has also been considered. In ejaculates from normozoospermic patients with implantation failure, aneuploid, apoptotic and DNA-injured spermatozoa decreased significantly after MACS [110]. However, the interactions between apoptotic markers, DNA integrity and aneuploidy and the effect of MACS on these parameters remain unknown [110].…”

Section: In Vitro Studies On the Quality Of Sperm Selected By Macsmentioning

confidence: 99%

“…In ejaculates from normozoospermic patients with implantation failure, aneuploid, apoptotic and DNA-injured spermatozoa decreased significantly after MACS [110]. However, the interactions between apoptotic markers, DNA integrity and aneuploidy and the effect of MACS on these parameters remain unknown [110]. Other study was conducted in order to determine the fertilization potential of selected sperm (n=35) by hamster oocyte penetration and hamster oocyte-intracytoplasmic sperm injection [111].…”

Section: In Vitro Studies On the Quality Of Sperm Selected By Macsmentioning

confidence: 99%

Use of Annexin V based Sperm Selection in Assisted Reproduction

Teijeiro¹,

Munuce²,

Caille³

et al. 2017

Andrology

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Innovative methods to select sperm subpopulations with the best fertilizing ability are needed in assisted reproductive techniques (ART) in order to improve fertilization and pregnancy rates, while also considering possible epigenetic effects on the offspring. Molecular based selection methods are searched for, under the premise that they could be an improvement over classical selection by morphology and movement. One of these methods sustains the elimination of sperm that can bind to annexin A5 (ANX V), coupled to paramagnetic beads, through the phosphatidyl-serine exposed on their membranes upon apoptosis. Although reports accumulate about the use of this method, controversy persists as to the benefits of ANX V based sperm selection in ART. In this review we consider the arguments in favour and against this method and conclude that to the moment the evidence does not support MACS regular use in ART.

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Correlation between aneuploidy, apoptotic markers and DNA fragmentation in spermatozoa from normozoospermic patients

Cited by 35 publications

References 81 publications

Effect of Seminal Transforming Growth Factorß1 (TGFß1) and Glutathione on Apoptosis in Spermatozoa from Tunisian Infertile Men

Effect of Seminal Transforming Growth Factorß1 (TGFß1) and Glutathione on Apoptosis in Spermatozoa from Tunisian Infertile Men

Analysis of Sperm DNA Fragmentation and Aneuploidy in 109 Infertile Patients: Are the Two Parameters Correlated?

Use of Annexin V based Sperm Selection in Assisted Reproduction

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