Correlation between age and the secretions of melanocyte-stimulating cytokines in cultured keratinocytes and fibroblasts

Okazaki, Mutsumi; Yoshimura, Kotaro; Uchida, Gentaro; Harii, Kiyonori

doi:10.1111/j.1365-2133.2005.06966.x

Cited by 53 publications

(40 citation statements)

References 21 publications

(37 reference statements)

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“…To our knowledge, no correlation between age and SCF secretion by normal fibroblasts has been established [17]. The decreased number of CLS may partly be related to the decrease with age in the number of melanocytes or to SCF secretion impairment in NF1 patients [18].…”

Section: Discussionmentioning

confidence: 99%

Evolving Pattern with Age of Cutaneous Signs in Neurofibromatosis Type 1: A Cross-Sectional Study of 728 Patients

Duong

Bastuji‐Garin²,

Valeyrie‐Allanore

et al. 2011

Dermatology

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Background: Neurofibromatosis type 1 is fully penetrant by the age of 8 years, and 3 criteria of diagnosis are dermatological: café-au-lait spots (CLS), intertriginous freckling and neurofibromas (NF). Objectives: The aim of our study was to determine the evolving pattern of cutaneous manifestations during adulthood. Methods: Phenotypic data of patients seen in our center between March 2003 and December 2009 were studied. Patients were classified in 10-year groups. Following clinical characteristics, the number of CLS and the number of cutaneous and subcutaneous NF were compared according to age. Results: 728 subjects, 404 females and 324 males (mean age of 32.4 years, range 6–80 years) were studied. Four hundred eighty-nine patients were over 20 years old (67%). The number of CLS (small or large) was significantly decreased with age while the number of cutaneous and subcutaneous NF was strongly increased (p < 0.001). Conclusions: The decrease in CLS with age has not been previously reported while an increase in the number of NF is well described during puberty and pregnancy and with age.

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Section: Discussionmentioning

confidence: 99%

Evolving Pattern with Age of Cutaneous Signs in Neurofibromatosis Type 1: A Cross-Sectional Study of 728 Patients

Duong

Bastuji‐Garin²,

Valeyrie‐Allanore

et al. 2011

Dermatology

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“…It is also the general consensus of many single protein studies and functional characterizations that focus directly on keratinocytes and attempt to link the aging skin phenotype to a decline of protective biological functions. In these studies cytokine related defects in skin pigmentation could be excluded (72), as well as altered DNArepair mechanisms upon UV-radiation (73), and altered antioxidative capacity (74). Tissue integrity (75), DNA-replication (73), proliferation (76), differentiation, and expression of related cytokines (77) were also shown to be unaffected by aging in keratinocytes.…”

Section: A431 Cells Exhibit Decrease In Stromal Communication Increamentioning

confidence: 99%

Consistency of the Proteome in Primary Human Keratinocytes With Respect to Gender, Age, and Skin Localization

Sprenger

Weber

Zarai

et al. 2013

Molecular & Cellular Proteomics

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Keratinocytes account for 95% of all cells of the epidermis, the stratified squamous epithelium forming the outer layer of the skin, in which a significant number of skin diseases takes root. Immortalized keratinocyte cell lines are often used as research model systems providing standardized, reproducible, and homogenous biological material. Apart from that, primary human keratinocytes are frequently used for medical studies because the skin provides an important route for drug administration and is readily accessible for biopsies. However, comparability of these cell systems is not known. Cell lines may undergo phenotypic shifts and may differ from the in vivo situation in important aspects. Primary cells, on the other hand, may vary in biological functions depending on gender and age of the donor and localization of the biopsy specimen. Here we employed metabolic labeling in combination with quantitative mass spectrometry-based proteomics to assess A431 and HaCaT cell lines for their suitability as model systems. Compared with cell lines, comprehensive profiling of the primary human keratinocyte proteome with respect to gender, age, and skin localization identified an unexpected high proteomic consistency. The data were analyzed by an improved ontology enrichment analysis workflow designed for the study of global proteomics experiments. It enables a quick, comprehensive and unbiased overview of altered biological phenomena and links experimental data to literature. We guide through our workflow, point out its advantages compared with other methods and apply it to visualize differences of cell lines compared with primary human keratinocytes. Molecular &

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“…KGF-1, a member of the FGF family that is secreted by fibroblasts [Okazaki et al, 2002[Okazaki et al, , 2005, is a potent mediator of keratinocyte proliferation and migration [Cross and Mustoe, 2003]. Both keratinocytes and fibroblasts are important sources of bFGF (FGF-2), which stimulates proliferation, differentiation and migration of regenerated keratinocytes, aids angiogenesis by stimulating proliferation of capillary endothelial cells and promotes migration of fibroblasts [Bennet et al, 2003].…”

Section: Detection Of Selected Growth Factorsmentioning

confidence: 99%

Nonirradiated Human Fibroblasts and Irradiated 3T3-J2 Murine Fibroblasts as a Feeder Layer for Keratinocyte Growth and Differentiation in vitro on a Fibrin Substrate

Panacchia¹,

Dellambra²,

Bondanza³

et al. 2009

Cells Tissues Organs

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The standard method for producing graftable epithelia relies on the presence of a feeder layer of lethally irradiated 3T3-J2 murine fibroblasts (Rheinwald and Green technique). Here, we studied a new keratinocyte culture system, which envisages the utilization of nonirradiated human fibroblasts embedded into a fibrin substrate, in cultures destined for a future clinical application. We tested this culture system using keratinocytes grown on a fibrin gel precoated with 3T3-J2 murine fibroblasts as a control. In order to evaluate the new technology, we compared the clonogenic potential and the proliferative, differentiative and metabolic characteristics of keratinocytes cultured on the fibrin gel under the two culture conditions. The results demonstrated that the proposed technology did not impair the behavior of cultured keratinocytes and revealed that cells maintained their proliferative potential and phenotype under the experimental conditions. In particular, the demonstration of stem cell maintenance under the adopted culture conditions is very important for acute burn treatment with skin substitutes. This work is a first step in the evaluation of a new keratinocyte culture system, which has been studied in order to take advantage of an additional human cell population (i.e. nonirradiated, growing fibroblasts) for future transplantation purposes in acute and chronic wounds. Additional research will allow us to attain (1) the removal of murine cells in the initial phase of keratinocyte cultures, and (2) the removal of other potentially dangerous animal-derived materials from the entire culture system.

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Correlation between age and the secretions of melanocyte-stimulating cytokines in cultured keratinocytes and fibroblasts

Cited by 53 publications

References 21 publications

Evolving Pattern with Age of Cutaneous Signs in Neurofibromatosis Type 1: A Cross-Sectional Study of 728 Patients

Evolving Pattern with Age of Cutaneous Signs in Neurofibromatosis Type 1: A Cross-Sectional Study of 728 Patients

Consistency of the Proteome in Primary Human Keratinocytes With Respect to Gender, Age, and Skin Localization

Nonirradiated Human Fibroblasts and Irradiated 3T3-J2 Murine Fibroblasts as a Feeder Layer for Keratinocyte Growth and Differentiation in vitro on a Fibrin Substrate

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