Correlation analysis between the karyotypes and phenotypic traits of Chinese cymbidium cultivars

Huijuan, Ning; Ao, Suyan; Fan, Yining; Fu, Jianxin; Xu, Chao

doi:10.1007/s13580-018-0010-6

Cited by 7 publications

(7 citation statements)

References 17 publications

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“…The flipside of horticultural popularity is that C . goeringii is currently threatened by overexploitation and habitat disturbance and the fragmentation of its natural habitats in China, Japan, and Korea (Leitch et al, 2009; Ning et al, 2018). Generating high‐quality genomic resources in C .…”

Section: Introductionmentioning

confidence: 99%

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A chromosome‐scale genome assembly and annotation of the spring orchid (Cymbidium goeringii)

Chung

Kim

Bolser

et al. 2021

Molecular Ecology Resources

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Cymbidium goeringii, commonly known as the spring orchid, has long been favoured for horticultural purposes in Asian countries. It is a popular orchid with much demand for improvement and development for its valuable varieties. Until now, its reference genome has not been published despite its popularity and conservation efforts. Here, we report the de novo assembly of the C. goeringii genome, which is the largest among the orchids published to date, using a strategy that combines short‐ and long‐read sequencing and chromosome conformation capture (Hi‐C) information. The total length of all scaffolds is 3.99 Gb, with an N50 scaffold size of 178.2 Mb. A total of 29,556 protein‐coding genes were annotated and 3.55 Gb (88.87% of genome) repetitive sequences were identified. We constructed pseudomolecular chromosomes using Hi‐C, incorporating 89.4% of the scaffolds in 20 chromosomes. We identified 220 expanded and 106 contracted genes families in C. goeringii after divergence from its close relative. We also identified new gene families, resistance gene analogues and changes within the MADS‐box genes, which control a diverse set of developmental processes during orchid evolution. Our high quality chromosomal‐level assembly of C. goeringii can provide a platform for elucidating the genomic evolution of orchids, mining functional genes for agronomic traits and for developing molecular markers for accelerated breeding as well as accelerating conservation efforts.

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Section: Introductionmentioning

confidence: 99%

“…The estimated C . goeringii genome size is 4 Gb, consisting of 20 chromosomes (Ning et al, 2018). We found out that the spring orchid genome possesses a large number of repeat sequences compared to other orchid genomes.…”

Section: Introductionmentioning

confidence: 99%

A chromosome‐scale genome assembly and annotation of the spring orchid (Cymbidium goeringii)

Chung

Kim

Bolser

et al. 2021

Molecular Ecology Resources

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“…Cymbidium Sw. is one of the most important orchids consisting of 74 species that are epiphytic, lithophytic, terrestrial or sometimes rarely leafless saprophytic (Ning et al, 2018;Thakur and Dutt, 2021). Among the terrestrial species, C. sinense, C. ensifolium, C. goeringi, and C. kanran are the most popular flowering ornamental plants and widely cultivated for their beauty and fragrance (Huang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants

Su²,

et al. 2022

Front. Plant Sci.

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Triploid is considered a reproductive barrier and also a bridge in the formation of polyploids. However, few reports are available in Cymbidium. In this study, diploid ‘Xiaofeng’, sexual triploid ‘Yuchan’ and ‘Huanghe’ of Cymbidium were used to evaluate hybridization compatibility of the triploids. Results showed that the sexual triploids were fertile whether they were used as male or female parents. ‘Yuchan’ produced male gametes of 1x, 1x~2x, 2x, 2x~3x, and 3x at frequencies of 8.89%, 77.78%, 6.67%, 3.33%, and 3.33%, respectively; while ‘Huanghe’ produced 3.33% 1x, 80.00% 1x~2x, 8.89% 2x, 5.56% 2x~3x, and 2.22% 3x male gametes. The cross of ‘Xiaofeng’ with ‘Yuchan’ produced progenies with a wide range of ploidy levels, including one diploid, 34 2×~3× aneuploids, 12 triploids, and one tetraploid, indicating that male gametes produced by sexual triploid were fertile and could be transmitted and fused with egg cells. On the other hand, 10 progenies obtained from the cross of ‘Yuchan’ × ‘Xiaofeng’ were all aneuploids. The cross of ‘Yuchan’ with ‘Huanghe’ produced 40 progenies including three 2×~3× aneuploids, nine 3×~4× aneuploids, 21 tetraploids, six 4×~5× aneuploids, and one pentaploid, suggesting that 2x gametes, instead of the unreduced ones played a more important role in the formation of tetraploids. The survival rates of the hybrids were all above 80.00%, with the tetraploids at 96.67%. Cytological analysis revealed that during meiosis of sexual polyploids, two chromosome sets of the 2n gamete were inclined to enter into the same daughter cell, resulting in the production of 2x gametes. Our results indicate that the triploid cymbidiums are not reproductive barrier but serve as a bridge in the formation of polyploid plants.

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“…Due to the natural hybridization of the Cymbidium species in the nature, there are many intermediate types, making the boundaries of this species unclear (Ning et al. 2018 ). Today, the classification of Cymbidium species is mainly based on morphological indicators (Jiaping and Silan 1998 ).…”

mentioning

confidence: 99%

“…Meanwhile, there is also a lack of DNA data of the Cymbidium species, which is one of the most important tools in taxonomy (Ning et al. 2018 ). Therefore, it is urgent to provide valuable genetic information for this species.…”

mentioning

confidence: 99%

The complete chloroplast genome of Cymbidium longibracteatum (Orchidaceae) and phylogenetic analysis

Huijuan

Shao²,

Dai

2020

Mitochondrial DNA Part B

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Correlation analysis between the karyotypes and phenotypic traits of Chinese cymbidium cultivars

Cited by 7 publications

References 17 publications

A chromosome‐scale genome assembly and annotation of the spring orchid (Cymbidium goeringii)

A chromosome‐scale genome assembly and annotation of the spring orchid (Cymbidium goeringii)

Triploid cultivars of Cymbidium act as a bridge in the formation of polyploid plants

The complete chloroplast genome of Cymbidium longibracteatum (Orchidaceae) and phylogenetic analysis

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