Correlation analysis between serum NETs, FIB, miR-374a-5p levels and deep vein thrombosis in the lower limb after knee arthroplasty

Abstract

Objective To investigate the changes in the levels of serum neutrophil extracellular traps (NETs), fibrinogen (FIB), and micro RNA-374a-5p (miR-374a-5p) after knee arthroplasty, and to analyze their correlation with postoperative deep vein thrombosis (DVT) and their predictive efficacy. Methods 108 patients who underwent knee arthroplasty in Nanjing Pukou People's Hospital from September 2020 to May 2023 were selected for the study, and 32 patients in the DVT group and 76 patients in the non-DVT group were categorized according to the occurrence of lower limb DVT at 7 days after surgery. The clinical data of the two groups and the levels of serum NETs, FIB and miR-374a-5p in the preoperative period, 1 day postoperative period and 3 days postoperative period were compared and analyzed. Pearson's method was used to analyze the correlation between preoperative serum NETs, FIB, miR-374a-5p levels and the venous thromboembolism risk assessment scale (Caprini) score. Multifactorial logistic regression was used to analyze the factors influencing the occurrence of lower limb DVT after surgery. The predictive value of serum NETs, FIB, and miR-374a-5p levels on the occurrence of postoperative lower limb DVT was evaluated by using receiver operating characteristic curve(ROC) and area under the curve (AUC). Results The levels of serum NETs, FIB, and miR-374a-5p were higher in the DVT group than in the non-DVT group at 1 day and 3 days postoperatively (P<0.05); the levels of preoperative serum NETs, FIB, and miR- 374a-5p were positively correlated with the Caprini score (P<0.05); The Caprini score and elevated levels of serum NETs,FIB,and miR-374a-5p at 3 days after surgery were independent risk factors for postoperative lower limb DVT (P<0.05);The combined prediction of serum NETs,FIB,and miR-374a-5p levels at 3 days after surgery for AUC in lower limb DVT was greater than that predicted by single indicators ( P<0.05). Conclusion The levels of serum NETs,FIB, and miR-374a-5p in patients with DVT after knee arthroplasty increase.Combined detection of their levels has certain predictive value for the occurrence of postoperative lower limb DVT.