Correlating two methods of quantifying fungal activity: Heat production by isothermal calorimetry and ergosterol amount by gas chromatography–tandem mass spectrometry

Li, Yujing; Wadsö, Lars; Larsson, Lars; Bjurman, Jonny

doi:10.1016/j.tca.2007.01.005

Cited by 20 publications

(16 citation statements)

References 43 publications

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“…In the present measurements, different amounts of heat were produced to make biomass at different temperatures. In an earlier study (Li et al 2007), we found four times higher heat production per unit ergosterol at 15°C compared with 20°C. In this study with a much improved methodology, the same ratio is about 1AE6.…”

Section: Discussionmentioning

confidence: 50%

“…Heat production by mould growth was measured by isothermal calorimetry as in our previous studies (Wadsö et al 2004;Li et al 2007). The thermal power measured from biological samples is produced by the biochemical reactions of their metabolism.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we studied the impact of temperature on Penicillium roqueforti by comparing its produced heat, ergosterol and biomass during its growth phase at different temperature levels. Heat production by mould growth was measured by isothermal calorimetry as in our previous studies (Wadsö et al 2004;Li et al 2007). The thermal power measured from biological samples is produced by the biochemical reactions of their metabolism.…”

Section: Introductionmentioning

confidence: 99%

“…Over the years, more and more sensitive calorimeters have been used in the biological field to study 'from macromolecules to man' (Kemp 1999). Except for measurements on yeasts and complex samples such as soils and foodstuffs that may contain fungi, there have been only a few calorimetric studies conducted on fungi (Wadsö 1997;Xie et al 1997;Markova and Wadsö 1998;Bjurman and Wadsö 2000;Wadsö et al 2004;Li et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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Impact of temperature on growth and metabolic efficiency ofPenicillium roqueforti- correlations between produced heat, ergosterol content and biomass

Wadsö

Larsson

2009

Journal of Applied Microbiology

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The influence of temperature on the growth of the mould Penicillium roqueforti growing on malt extract agar was studied by correlating the produced heat (measured by isothermal calorimetry), ergosterol content (quantified by GC‐MS/MS) and biomass of the mould at 10, 15, 20, 25 and 30°C. The results were analysed with a simple metabolic model from which the metabolic efficiency was calculated. The results show that the impact of temperature on growth rate and metabolic efficiency are different: although the mould fungus had the highest growth rate (in terms of thermal power, which was continuously measured) at 25°C, the substrate carbon conversion efficiency (biomass production divided by substrate consumption, both counted as moles carbon) was the highest at 20°C. The temperature of the most rapid growth did not therefore equal the temperature of the most efficient growth.

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Section: Discussionmentioning

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of temperature on growth and metabolic efficiency ofPenicillium roqueforti- correlations between produced heat, ergosterol content and biomass

Wadsö

Larsson

2009

Journal of Applied Microbiology

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“…During the exponential growth, the measured thermal power is proportional to the rate of increase in the biomass, but the thermal power can also-in later growth phases-come mainly from maintenance processes (Li, Wadsö, Larsson, & Bjurman, 2007). Generally speaking, the thermal power from an organism can be measured and used as an indicator of the organism's activity level (Wadsö & Gómez Galindo, 2009).…”

Section: Ph Dough Volume and Heat Productionmentioning

confidence: 99%

Physico-chemical and metabolomic characterization of KAMUT® Khorasan and durum wheat fermented dough

et al. 2015

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The relative importance of metabolic rate and body size to space use behavior in aquatic invertebrates

Shokri,

Marrocco,

Cozzoli

et al. 2024

Ecology and Evolution

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Elucidating the underlying mechanisms behind variations of animal space and resource use is crucial to pinpoint relevant ecological phenomena. Organism's traits related to its energy requirements might be central in explaining behavioral variation, as the ultimate goal of a forager is to fulfill its energy requirements. However, it has remained poorly understood how energy requirements and behavioral patterns are functionally connected. Here we aimed to assess how body mass and standard metabolic rate (SMR) influence behavioral patterns in terms of cumulative space use and time spent in an experimental patchy environment, both within species and among individuals irrespective of species identity. We measured the behavioral patterns and SMR of two invertebrate species, that is, amphipod Gammarus insensibilis, and isopod Lekanesphaera monodi, individually across a range of body masses. We found that species of G. insensibilis have higher SMR level, in addition to cumulatively exploring a larger space than L. monodi. Cumulative space use scaled allometrically with body mass, and it scaled isometrically with SMR in both species. While time spent similarly in both species was characterized by negative body mass and SMR dependence, it was observed that L. monodi individuals tended to stay longer in resource patches compared to G. insensibilis individuals. Our results further showed that within species, body mass and metabolic rate explained a similar amount of variation in behavior modes. However, among individuals, regardless of species identity, SMR had stronger predictive power for behavioral modes compared to body mass. This suggests that SMR might offer a more generalized and holistic description of behavioral patterns that extend beyond species identity. Our study on the metabolic and body mass scaling of space and resource use behavior sheds light on higher‐order ecological processes such as species' competitive coexistence along the spatial and trophic dimensions.

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Correlating two methods of quantifying fungal activity: Heat production by isothermal calorimetry and ergosterol amount by gas chromatography–tandem mass spectrometry

Cited by 20 publications

References 43 publications

Impact of temperature on growth and metabolic efficiency ofPenicillium roqueforti- correlations between produced heat, ergosterol content and biomass

Impact of temperature on growth and metabolic efficiency ofPenicillium roqueforti- correlations between produced heat, ergosterol content and biomass

Physico-chemical and metabolomic characterization of KAMUT® Khorasan and durum wheat fermented dough

The relative importance of metabolic rate and body size to space use behavior in aquatic invertebrates

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