Correlating sea lamprey density with environmental DNA detections in the lab

Schloesser, Nicholas A.; Merkes, Christopher M.; Rees, Christopher B.; Amberg, Jon J.; Steeves, Todd B.; Docker, Margaret F.

doi:10.3391/mbi.2018.9.4.11

Cited by 20 publications

(15 citation statements)

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“…Usually, eDNA shedding rate is presumed to be highest during the time the organisms occupy the site, but not always; see Schloesser et al. (). Schloesser et al.…”

Section: Discussionmentioning

confidence: 99%

“…Schloesser et al. () found eDNA concentrations increased in the spring when Sea Lamprey began migrating into streams to mate and remained high after spawning was done, likely due to carcasses shedding large amounts of DNA over time. Additionally, Schloesser et al.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, Schloesser et al. () found significant eDNA concentrations at a site with no Sea Lamprey present likely due to a population located somewhere upstream of the site. If less is known about habitat use and timing of habitat use, more visits to a site over time would be more informative for sites that are not occupied regularly.…”

Section: Discussionmentioning

confidence: 99%

“…eDNA occurrence at a sampling site is dependent upon the eDNA accumulation rate, which is linked to organisms shedding rate vs. the rates of eDNA degradation, dilution, or flow out of the site. Usually, eDNA shedding rate is presumed to be highest during the time the organisms occupy the site, but not always; see Schloesser et al (2018). Schloesser et al (2018) found eDNA concentrations increased in the spring when Sea Lamprey began migrating into streams to mate and remained high after spawning was done, likely due to carcasses shedding large amounts of DNA over time.…”

Section: Discussionmentioning

confidence: 99%

“…As part of ongoing management, these programs seek to improve and innovate sampling strategies by incorporating new tools and methods as they become available. For example, the GLFC continuously investigates the use of new technologies (Jones 2007) such as environmental DNA (eDNA)-based monitoring approaches (Gingera et al 2016, Schloesser et al 2018.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Refinement of eDNA as an early monitoring tool at the landscape‐level: study design considerations

Mize

Erickson²,

Merkes³

et al. 2019

Ecological Applications

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Natural resource managers use data on the spatial range of species to guide management decisions. These data come from survey or monitoring efforts that use a wide variety of tools. Environmental DNA (eDNA) is a surveillance tool that uses genetic markers for detecting species and holds potential as a tool for large‐scale monitoring programs. Two challenges of eDNA‐based studies are uncertainties created by imperfect capture of eDNA in collection samples (e.g., water field samples) and imperfect detection of eDNA using molecular methods (e.g., quantitative PCR). Occurrence models can be used to address these challenges, thus we use an occurrence model to address two objectives: first, to determine how many samples were required to detect species using eDNA; second, to examine when and where to take samples. We collected water samples from three different habitat types in the Upper Mississippi River when both Bighead Carp and Silver Carp were known to be present based on telemetry detections. Each habitat type (backwater, tributary, and impoundment) was sampled during April, May, and November. Detections of eDNA for both species varied across sites and months, but were generally low, 0–19.3% of samples were positive for eDNA. Overall, we found that eDNA‐based sampling holds promise to be a powerful monitoring tool for resource managers; however, limitations of eDNA‐based sampling include different biological and ecological characteristics of target species such as seasonal habitat usage patterns as well as aspects of different physical environments that impact the implementation of these methods such as water temperature.

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“…Usually, eDNA shedding rate is presumed to be highest during the time the organisms occupy the site, but not always; see Schloesser et al. (). Schloesser et al.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Refinement of eDNA as an early monitoring tool at the landscape‐level: study design considerations

Mize

Erickson²,

Merkes³

et al. 2019

Ecological Applications

View full text Add to dashboard Cite

show abstract

Evaluation of environmental DNA surveys for identifying occupancy and spatial distribution of Pacific Lamprey (Entosphenus tridentatus) and Lampetra spp. in a Washington coast watershed

et al. 2019

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Surveys of environmental DNA (eDNA) have become an important and multifaceted tool for monitoring and identifying distributions and occupancy of aquatic species. This tool is attractive because it is powerful, easy to apply, and provides an alternative to traditional field survey methods. However, validating eDNA survey methods against traditional field survey methods is warranted prior to their application. We used eDNA and electrofishing to survey 10 sites in 3 tributaries of the Chehalis River, Washington, to infer distribution and occupancy of Entosphenus tridentatus and Lampetra spp. Both methods produced similar detection rates for E. tridentatus, and Lampetra spp. were detected at slightly greater frequency with eDNA in the Black River and Skookumchuck River. Within each of the three tributaries, eDNA concentration was negatively related to sample distance from the Chehalis River mainstem for E. tridentatus but not for Lampetra spp., which indicates E. tridentatus and Lampetra spp. may be distributed differently within tributaries. Application of lamprey eDNA data to a multiscale occupancy model indicated high probability of detecting eDNA in water samples and quantitative PCR (qPCR) assays. Broad distribution and high detection of E. tridentatus and Lampetra spp. suggest robust populations inhabit the Chehalis River basin. Our findings suggest eDNA surveys may be comparable to electrofishing for informing lamprey occupancy and distributions. Such sampling is efficient and cost‐effective and we anticipate that eDNA surveys will become a valuable tool in addressing key research and monitoring needs for conservation and restoration of lampreys in general.

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Environmental DNA (eDNA) as a tool for assessing fish biomass: A review of approaches and future considerations for resource surveys

et al. 2021

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Environmental DNA (eDNA) has revolutionized our ability to identify the presence and distributions of terrestrial and aquatic organisms. Recent evidence suggests the concentration of eDNA could also provide a rapid, cost-effective indicator of abundance and/or biomass for fisheries stock assessments. Globally, fisheries resources are under immense pressure, and their sustainable harvest requires accurate information on the sizes of fished stocks. However, in many cases the required information remains elusive because of a reliance on imprecise or costly fishery-dependent and independent data. Here, we review the literature describing relationships between eDNA concentrations and fish abundance and/or biomass, as well as key influencing factors, as a precursor to determining the broader utility of eDNA for monitoring fish populations.We reviewed 63 studies published between 2012 and 2020 and found 90% identified positive relationships between eDNA concentrations and the abundance and/or biomass of focal species. Key influencing biotic factors included the taxon examined as well as their body size, distribution, reproduction, and migration. Key abiotic factors mostly comprised hydrological processes affecting the dispersal and persistence of eDNA, especially water flow and temperature, although eDNA collection methods were also influential. The cumulative influence of these different factors likely explains the substantial variability observed in eDNA concentrations, both within and among studies. Nevertheless, there is considerable evidence to support using eDNA as an ancillary tool for assessing fish population abundance and/or biomass across discrete spatio-temporal scales, following preliminary investigations to determine speciesand context-specific factors influencing the eDNA abundance/biomass relationship.Advantages of eDNA monitoring relative to other approaches include reduced costs, increased efficiencies, and nonlethal sampling.

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Correlating sea lamprey density with environmental DNA detections in the lab

Cited by 20 publications

References 0 publications

Refinement of eDNA as an early monitoring tool at the landscape‐level: study design considerations

Refinement of eDNA as an early monitoring tool at the landscape‐level: study design considerations

Evaluation of environmental DNA surveys for identifying occupancy and spatial distribution of Pacific Lamprey (Entosphenus tridentatus) and Lampetra spp. in a Washington coast watershed

Environmental DNA (eDNA) as a tool for assessing fish biomass: A review of approaches and future considerations for resource surveys

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