Correlating Cell Morphology and Osteoid Mineralization Relative to Strain Profile for Bone Tissue Engineering Applications

Wood, Mairead A.; Yang, Ying; Baas, Elbert; Meredith, D.O.; Richards, Rebecca; Kuiper, Jan Herman; Haj, Alicia J. El

doi:10.1016/s0021-9290(08)70160-x

Cited by 1 publication

(2 citation statements)

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“…These cell morphologies indicate the fully differentiated osteoblasts embedded in the bone matrix. 35 Previously, we have reported that presence of small amount of BGN in coatings significantly improved the in vitro apatite-forming ability and osteogenic differentiation. 32 To further understand the impact of nanostructured surface topography on the cells, it was evaluated using fluorescence microscopy (Figure 8c).…”

Section: Characterization Of Mbgnmentioning

confidence: 94%

“…This result indicates that MBGN-Chi coatings are favorable coating surface to the growth of MC3T3-E1 cells without any cytotoxic effect. 35 . Previously, we have reported that presence of small amount of BGN in coatings significantly improved the in vitro apatite-forming ability and osteogenic differentiation 32 .…”

Section: Cell Adhesion Spreading and Proliferationmentioning

confidence: 99%

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Combined Effects of Nanoroughness and Ions Produced by Electrodeposition of Mesoporous Bioglass Nanoparticle for Bone Regeneration

Patel

Buitrago

Parthiban

et al. 2019

ACS Appl. Bio Mater.

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Providing appropriate biophysical and biochemical cues to the interface is a facile strategy to enhance the osteogenic ability of metallic implants. Here we exploited this through the incorporation of mesoporous bioactive glass nanoparticles (MBGN) at a high content (1:1 by weight) to a biopolymer chitosan in the electrodeposition process of titanium. The MGBN/chitosan layer thickness, tunable by electrodeposition parameters, exhibited an accelerated ability of apatite mineral induction in a body simulating medium. Of note, the involvement of MBGN could generate nanoscale roughness in a unique range of 10 ~ 25 nm.Moreover, the layer showed a slowly releasing profile of ions (calcium and silicate) over weeks at therapeutically relevant doses. The ion-releasing nanotopological surface was demonstrated to alter the pre-osteoblasts responses in a way favorable for the osteogenic differentiation. The combinatory cues of nanotopology (25 nm roughness) and ion release enabled highly accelerated cellular anchorage with somewhat limited spreading area at initial periods. The subsequent osteoblastic differentiation behaviors on the engineered surface, as examined up to 21 days, showed significantly enhanced alkaline phosphate activity and up-regulated expression of bone-associated genes (ALP, Col I, OPN, and OCN).These results indicate that the combinatory cues provided by nanotopology (25 nm roughness) and ions released from MBGN are highly effective in stimulating osteoblastic differentiation and that suggest the MBGN/chitosan may serve as a potential composition for bone implant coatings.

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Section: Characterization Of Mbgnmentioning

confidence: 94%

Section: Cell Adhesion Spreading and Proliferationmentioning

confidence: 99%