Correction: β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds

Dabouian, Atiyeh; Bakhshi, Hadi; Irani, Shiva; Pezeshki‐Modaress, Mohamad

doi:10.1039/c8ra90032a

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“…Their results showed that β-carotene embedded fibrous mats (polycaprolactone) were successfully providing a desirable environment for osteo-differentiation of seeded MSCs. 56 In this respect, based on the immunocytochemistry results of our study, the expression of SC-specific markers S100 and P75 ntr in ADSC/PDMS/β-carotene was elevated to roughly 84% and 75%, respectively. Moreover, q-real time PCR evaluations quantitatively confirmed their increase at a significant level (p-value<0.001).…”

Section: Immunocytochemistry Analysismentioning

confidence: 57%

P75 and S100 gene expression induced by cell‐imprinted substrate and beta‐carotene to nerve tissue engineering

Dadashkhan

Irani

Bonakdar

et al. 2021

J of Applied Polymer Sci

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Here we aimed to differentiate adipose derived stem cells (ADSCs) to Schwann cells (SCs), as one of the major and instrumental cell sources in nerve regeneration, by synergistic application of imprinting method and β-carotene. Accordingly, the topography of Schwann cells was imprinted on poly dimethyl siloxane (PDMS) substrates via mold casting and human ADSCs seeded on substrates; moreover, β-carotene was added to induce hADSCs differentiation. Physiochemical evaluations of PDMS by FTIR spectra presented its siliconmethyl bond (Si CH 3) at 1260 cm −1. Morphology analysis by crystal violet, picrosirus red staining, and SEM images illustrated that MSCs seeded on imprinted substrates have formed SC-like morphology. Furthermore, according to q-PCR and ICC evaluations, SCs specific markers; S100 and P75 in addition of 5 μl β-carotene (BC) were upregulated (p-value<0.001). Also, the expression was detected on the imprinted surfaces without β-carotene to a lesser degree. Our study revealed that Schwann cell imprinted substrates can mimic the morphology and topography of SCs and induce differentiation signals in mesenchymal stem cells (MSCs). In addition, the potency of β-carotene as an organic substance in boosting and stimulating the neural differentiation was demonstrated. Relevantly, the reports have confirmed the synergistic pivotal roles of β-carotene and patterned surfaces in directing MSCs into SC-like cells differentiation without applying expensive and less safe chemical growth factors.

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Section: Immunocytochemistry Analysismentioning

confidence: 57%

P75 and S100 gene expression induced by cell‐imprinted substrate and beta‐carotene to nerve tissue engineering

Dadashkhan

Irani

Bonakdar

et al. 2021

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

show abstract

Correction: β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds

Abstract: Correction for ‘β-Carotene: a natural osteogen to fabricate osteoinductive electrospun scaffolds’ by Atiyeh Dabouian et al., RSC Adv., 2018, 8, 9941–9945.

Cited by 1 publication

References 0 publications

P75 and S100 gene expression induced by cell‐imprinted substrate and beta‐carotene to nerve tissue engineering

P75 and S100 gene expression induced by cell‐imprinted substrate and beta‐carotene to nerve tissue engineering

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