Correction: Variations of Plasmid Content in Rickettsia felis

Fournier, Pierre-Édouard; Belghazi, Lokmane; Robert, Caroline; Elkarkouri, Khalid; Richards, Allen L.; Greub, Gilbert; Collyn, François; Ogawa, Motohiko; Portillo, Arantxa; Oteo, José Antonio; Psaroulaki, Anna; Bitam, Idir; Raoult, Didier

doi:10.1371/annotation/6870e918-77d7-4ef5-ae5e-6cf994f9c169

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, we could not detect the pRFδ plasmid sequence by PCR (primers pRFa and pRFd) in any of our R. felis Danube samples. Similar findings were described for the R. felis strains LSU and LSU-Lb [ 55 , 56 ], and the R. felis genotype RF2125 endemic in Archaeopsylla erinacei fleas from Algeria [ 97 ], confirming plasmid content variation between different R. felis strains [ 81 ].…”

Section: Resultssupporting

confidence: 79%

“…To amplify a portion of the R. felis pRF and pRFδ plasmid sequences, we used oligonucleotides published previously [ 81 ]. The PCR reaction was composed of Platinum Taq DNA High Fidelity Polymerase (1 U per reaction; Invitrogen, Waltham, MA, USA), High Fidelity PCR Buffer, 2.0 mM MgSO 4 , 0.2 mM of each dNTP, 0.4 µM of each primer, ultrapure nuclease-free water, and 2 µL of template DNA.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Culture Isolate of Rickettsia felis from a Tick

Danchenko

Benada

Škultéty

et al. 2022

IJERPH

View full text Add to dashboard Cite

Although the cat flea, Ctenocephalides felis, has been identified as the primary vector of Rickettsia felis, additional flea, tick, mite, and louse species have also been associated with this bacterium by molecular means; however, the role of these arthropods in the transmission of R. felis has not been clarified. Here, we succeeded in culture isolation of R. felis from a host-seeking castor bean tick, Ixodes ricinus, the most common tick in Slovakia. The bacterial isolation was performed on XTC-2 cells at 28 °C using the shell-vial technique. An evaluation of the growth properties was performed for both the XTC-2 and Vero cell lines. We observed R. felis in the infected host cells microscopically by Gimenez staining and immunofluorescence assay. The R. felis isolate was purified by gradient ultracentrifugation and visualized by electron microscopy. Fragments of the genes gltA, ompA, ompB, htrA, rpoB, sca4, rffE, and rrs were amplified and compared with the corresponding sequences of the type strain URRWXCal2 and other R. felis culture -isolated strains. We did not detect any nucleotide polymorphisms; however, plasmid pRFδ, characteristic of the standard strain, was absent in our isolate. Herein, we describe the first successful isolation and characterization of a tick-derived R. felis strain “Danube”, obtained from an I. ricinus nymph.

show abstract

Section: Resultssupporting

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Culture Isolate of Rickettsia felis from a Tick

Danchenko

Benada

Škultéty

et al. 2022

IJERPH

View full text Add to dashboard Cite

show abstract

Genetic Diversity of Xylella fastidiosa Plasmids Assessed by Comparative Genomics

Pierry

Uceda-Campos

Feitosa-Junior

et al. 2020

Trop. plant pathol.

View full text Add to dashboard Cite

Plasmids are a major source of horizontal gene transfer among bacteria contributing to their evolution and ecology. The known collection of plasmids carried by the plant pathogen Xylella fastidiosa increases as new strains from different origins and hosts are sampled and sequenced. Here we performed an extensive comparison of 61 publicly available sequences annotated as plasmids belonging to 38 X. fastidiosa strains isolated from different plant species and distinct geographical locations. Twenty-six strains exhibited at least one plasmid and up to four plasmids were found in a single strain. Plasmids sizes varied widely from 1.3 kbp to 64.3 kbp, ranging from 1 to 70 protein-coding sequences (CDS) encompassing 324 orthologs. Based on the presence of specific mobility proteins such as relaxases and type 4 secretion system-related genes, respectively 40 and 8 of the X. fastidiosa plasmids were classified as conjugative and mobilizable, while 13 were classified as non-mobilizable. X. fastidiosa plasmids did not carry known antibiotic resistance or virulence genes, and their stability seems to take advantage of toxin/antitoxin systems. The comparative analyses described here revealed similarity among plasmids of X. fastidiosa from different subspecies, geographical regions, and hosts, as well as with sequences found in plasmids from other bacterial species. Altogether, our results provide an in silico analysis of X. fastidiosa plasmid content and their main features, with applications in future studies of epidemiology, ecology, and evolution of this phytopathogen.

show abstract

Phylogenetic Variants of Rickettsia africae, and Incidental Identification of "Candidatus Rickettsia Moyalensis" in Kenya

et al. 2016

View full text Add to dashboard Cite

BackgroundRickettsia africae, the etiological agent of African tick bite fever, is widely distributed in sub-Saharan Africa. Contrary to reports of its homogeneity, a localized study in Asembo, Kenya recently reported high genetic diversity. The present study aims to elucidate the extent of this heterogeneity by examining archived Rickettsia africae DNA samples collected from different eco-regions of Kenya.MethodsTo evaluate their phylogenetic relationships, archived genomic DNA obtained from 57 ticks a priori identified to contain R. africae by comparison to ompA, ompB and gltA genes was used to amplify five rickettsial genes i.e. gltA, ompA, ompB, 17kDa and sca4. The resulting amplicons were sequenced. Translated amino acid alignments were used to guide the nucleotide alignments. Single gene and concatenated alignments were used to infer phylogenetic relationships.ResultsOut of the 57 DNA samples, three were determined to be R. aeschlimanii and not R. africae. One sample turned out to be a novel rickettsiae and an interim name of “Candidatus Rickettsia moyalensis” is proposed. The bonafide R. africae formed two distinct clades. Clade I contained 9% of the samples and branched with the validated R. africae str ESF-5, while clade II (two samples) formed a distinct sub-lineage.ConclusionsThis data supports the use of multiple genes for phylogenetic inferences. It is determined that, despite its recent emergence, the R. africae lineage is diverse. This data also provides evidence of a novel Rickettsia species, Candidatus Rickettsia moyalensis.

show abstract

Correction: Variations of Plasmid Content in Rickettsia felis

Cited by 3 publications

References 0 publications

Culture Isolate of Rickettsia felis from a Tick

Culture Isolate of Rickettsia felis from a Tick

Genetic Diversity of Xylella fastidiosa Plasmids Assessed by Comparative Genomics

Phylogenetic Variants of Rickettsia africae, and Incidental Identification of "Candidatus Rickettsia Moyalensis" in Kenya

Contact Info

Product

Resources

About