Correction: Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity

Chiu, Isaac M.; Barrett, Lee; Williams, Eric; Strochlic, David E.; Lee, Seungkyu; Weyer, Andy; Lou, Shan; Bryman, Gregory; Roberson, David P.; Ghasemlou, Nader; Piccoli, Cara; Ahat, Ezgi; Wang, Victor; Cobos, Enrique J.; Stucky, Cheryl L.; Ma, Qiufu; Liberles, Stephen D.; Woolf, Clifford J.

doi:10.7554/elife.06720

Cited by 5 publications

(2 citation statements)

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“…This allowed us to follow the differentiation dynamics of neural crest cells to PNS neurons during human embryogenesis. To classify subtype identity of neural crest cells and investigate sensory neurogenesis in human, we mapped cells using markers of the PNS that had previously been defined in other species (Chiu et al, 2014;Hockley et al, 2019;Li et al, 2016;Usoskin et al, 2015;Vermeiren et al, 2020;Zeisel et al, 2018). We established a classification scheme to subdivide cells into the three cell types characteristic of the E11.…”

Section: Classification Of Peripheral Nervous System Cellsmentioning

confidence: 99%

Single-cell transcriptome profiling of the human developing spinal cord reveals a conserved genetic programme with human-specific features

et al. 2021

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The spinal cord receives input from peripheral sensory neurons and controls motor output by regulating muscle innervating motor neurons. These functions are carried out by neural circuits comprising molecularly distinct neuronal subtypes generated in a characteristic spatial-temporal arrangement from progenitors in the embryonic neural tube. To gain insight into the diversity and complexity of cells in the developing human neural tube we used single cell mRNA sequencing to profile cervical and thoracic regions in four human embryos of Carnegie Stages (CS) CS12, CS14, CS17 and CS19 from Gestational Weeks 4-7. Analysis of progenitor and neuronal populations from the neural tube and dorsal root ganglia identified dozens of distinct cell types and facilitated the reconstruction of the differentiation pathways of specific neuronal subtypes. Comparison with mouse revealed the overall similarity of mammalian neural tube development while highlighting human specific features. These data provide a catalogue of gene expression and cell type identity in the human neural tube that will support future studies of sensory and motor control systems. The data can be explored at https://shiny.crick.ac.uk/scviewer/neuraltube/.

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Section: Classification Of Peripheral Nervous System Cellsmentioning

confidence: 99%

Single-cell transcriptome profiling of the human developing spinal cord reveals a conserved genetic programme with human-specific features

et al. 2021

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“…In the DRG, for example, Na v 1.8-Cre-recombinase expression occurs predominantly in nociceptive but not mechanoreceptive neurons allowing for manipulation of genes within this subset of nociceptors (Stirling et al, 2005). Other sensory neuron populations can be similarly targeted with other subtype-specific promoters (e.g., parvalbumin for proprioceptors, Ret for low-threshold cutaneous mechanoreceptors; Niu et al, 2013; Chiu et al, 2014). However, these promoters target only some DRG neurons and their expression in other tissues reduces their overall utility.…”

Section: Introductionmentioning

confidence: 99%

Advillin Is Expressed in All Adult Neural Crest-Derived Neurons

Hunter

Smaila

Lopes

et al. 2018

eNeuro

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Promoter-based genetic recombination (via, e.g., Cre-lox) is most useful when all cells of interest express a particular gene. The discovery that the actin-binding protein advillin is expressed in all somatic sensory neurons has been exploited repeatedly to drive DNA recombination therein, yet specificity of expression has not been well demonstrated. Here, we characterize advillin expression amongst sensory neurons and in several other neural and non-neural tissues. We first validate an advillin antibody against advillin knock-out tissue, advillin promoter-driven EGFP, and advillin mRNA expression. In the dorsal root ganglion (DRG), advillin is enriched in non-peptidergic nociceptors. We also show that advillin expression, and advillin promotor-driven EGFP and Cre-recombinase expression, occurs in multiple tissues including the dorsal habenula of the epithalamus, endocrine cells of the gut, Merkel cells in the skin, and most strikingly, throughout the autonomic nervous system (sympathetic, parasympathetic, and enteric neurons) in mice, rats, and non-human primates. In the mouse pelvic ganglion, advillin immunoreactivity is most intense in pairs of small neurons, and concentrated in spine-like structures on the axon initial segment contacted by sympathetic preganglionic axons. In autonomic targets (iris and blood vessels), advillin is distributed along cholinergic parasympathetic axons and in sympathetic varicosities. Developmentally, advillin expression is absent from sympathetics at postnatal day 4 but begins to emerge by day 7, accounting for previous reports (based on embryonic expression) of advillin's specificity to sensory neurons. These results indicate that caution is warranted in interpreting previous studies in which advillin-driven genomic editing is either constitutive or performed after postnatal day 4.

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