Correction: Traceless enzymatic synthesis of monodispersed hypermodified oligodeoxyribonucleotide polymers from RNA templates

Abstract

Correction for ‘Traceless enzymatic synthesis of monodispersed hypermodified oligodeoxyribonucleotide polymers from RNA templates’ by Marek Ondruš et al., Chem. Commun., 2022, 58, 11248–11251, https://doi.org/10.1039/D2CC03588J.

Interrogating Aptamer Chemical Space Through Modified Nucleotide Substitution Facilitated by Enzymatic DNA Synthesis

“…We next sought to evaluate the possibility of synthesizing modified DNA using nucleotides 2 – 5 under primer extension (PEX) reaction conditions [12b,34] . To do so, we first considered a system composed of the 71‐nucleotide template T1 and the 18‐nucleotide, 5’‐FAM‐labelled primer P1 (see Table 1).…”

“…Using the optimized conditions established with Vent ( exo − ), all modified sequences could be obtained in good to excellent yields (see Figure S7). Conversion of dsDNA to ssDNA is not an obvious undertaking since most methods lead to poor recovery yields or partial template contamination, particularly for sequences containing hydrophobic moieties [34] . In order to avoid presence of unmodified DNA template, we purified the products of the PEX reactions by denaturing gel electrophoresis followed by excision of the bands corresponding to modified ssDNA.…”

“…Conversion of dsDNA to ssDNA is not an obvious undertaking since most methods lead to poor recovery yields or partial template contamination, particularly for sequences containing hydrophobic moieties. [34] In order to avoid presence of unmodified DNA template, we purified the products of the PEX reactions by denaturing gel electrophoresis followed by excision of the bands corresponding to modified ssDNA. The oligonucleotides were then extracted into an elution buffer containing 0.5 M ammonium acetate and 10 mM magnesium acetate.…”

Controlled enzymatic synthesis of oligonucleotides

“…In parallel to controlled enzymatic synthesis of oligonucleotides, various biocatalytic approaches based on template-dependent polymerases have been reported. For instance, asymmetric PCR 112 , Nicking Enzyme Amplification Reaction 113,114 , and primer extension reactions followed by magnoseparation 115 or digestion 116 have been proposed. More recently, an elegant approach for the crafting of short, modified therapeutic oligonucleotides is based on the polymerization of modified nucleotides on a catalytic self-priming hairpin template (Fig.…”

Artificial nucleotide codons for enzymatic DNA synthesis

“…5,6 Similarly, polymerases can incorporate base-, phosphate-and sugar-modified nucleoside triphosphates into DNA, RNA, and XNAs. [7][8][9][10][11][12][13][14][15][16][17][18][19] Proteins and nucleic acids both benefit from such an increase in chemodiversity. In the case of nucleic acids, the co-polymerization of modified nucleoside triphosphates enables Darwinian selection experiments to raise functional nucleic acids with enhanced properties, 9,[20][21][22] expand the genetic alphabet with orthogonal base pairs, 12,[23][24][25] and functionally tag DNA and RNA.…”