“…After 96 h, 100 ng/µl recombinant PN (rPN) (Biovendor, Candler, NC, USA) was added, imaging was taken for determining tumour spheroid growth kinetics at days 4, 7, 10, 13 and 15 after tumour spheroid initiation (day 0). During tumour spheroid progression, invadopodia 20 of 3D cell invasion was monitored at 0, 24, 48 and 72 h. Images and data analyses of tumour spheroids were carried out by the CellSens Standard program on an inverted microscope model IX71 (Olympus, Waltham, MA, USA). The mean radius was used to calculate the volume with the formula 4/3πr3.…”