Correction to: Study of Structural, Electronic and Vibrational Properties of Porous Silicon with Different Porosity

Lachenani, H.; Larabi, A.; Gabouze, N.

doi:10.1007/s12633-019-00219-0

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“…Porous silicon (PSi) material has been used as highperformance solid substrate due to their large surface to volume ratio which allowed the attachment of a large number of molecules over a large surface in a small volume [15,16]. In addition, PSi is easily prepared either in powder [17] or wafer [18], it has an excellent biocompatibility [19] and has flexibility in controlling the surface chemistry [20,21] which make this material ideal for the immobilization of enzymes [22].…”

Section: Introductionmentioning

confidence: 99%

Acetylcholinesterase Modified Porous Silicon for Electrochemical Measurement of Total Active Immobilized Enzyme Amount and Effective Malathion Detection

Khaldi¹,

Sam²,

Gabouze³

2020

I2M

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An amperometric method to measure the total amount of active and inhibited immobilized Acetylcholinesterase enzyme (AChE) and the quantification of AChE inhibitors was realized. In this approach porous silicon (PSi) surface was used as a matrix for AChE immobilization and Boron doped polycrystalline diamant (BDPD) was used as working electrode for the electrochemical measurements. The covalent immobilization of AChE from electric eel was achieved on amine functionalized PSi surface previously decorated with Au particles. This surface is suitable for a stable attachment of AChE enzyme. The amperometric detection of AChE activity at Boron doped polycrystalline diamond electrode is based on the oxidation of thiocholine, the enzymatic reaction product of immobilized AChE in the presence of the substrate acetylthiocholine chlorid (ATCl) and this without the need to further modify the BDPD surface or the use of other reagents. The concentration of immobilized active AChE enzymes was estimated to Γ≈ 1.8 10 12 AChE cm -2 by means of a calibration curve. Michaelis constant was assessed with a Km of 4.3 10 -4 M. Finally, the electrochemical quantification and detection of Malathion shows a good linear calibration curve where the concentration of Malathion range is from 2 to 6 nM within 6 minutes after inhibitor addition.

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