Correction to: Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line

Wheeler, Nicolas J; Dinguirard, Nathalie; Marquez, Joshua; Gonzalez, Adrian; Zamanian, Mostafa; Yoshino, Timothy P.; Castillo, Maria G.

doi:10.1186/s13071-018-3135-7

Cited by 5 publications

(6 citation statements)

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“…The post-genomic publications reviewed here present a genome for the Bge cells ( Wheeler et al, 2018 ), proteomic studies on parasite-host interactions using in vitro assays ( Dinguirard et al, 2018 ; Wu et al, 2017 ), innate memory and antiparasitic responses ( Gourbal et al, 2018 ; Portet et al, 2018 ; Pinaud et al, 2019 ), the genetic basis of resistance ( Tennessen et al, 2017 ; Allan et al, 2017 ; Allan et al, 2018a, b), snail pheromones and neuropeptides ( Pila et al, 2017 ; Wang et al, 2019 ), attractants for miracidia ( Wang et al, 2019 ), and epigenetics ( Geyer et al, 2017 ; Queiroz et al, 2017 ).…”

Section: Post-genome Advancesmentioning

confidence: 99%

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Biomphalaria glabrata immunity: Post-genome advances

Castillo

Humphries

Mourão

et al. 2020

Developmental & Comparative Immunology

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Section: Post-genome Advancesmentioning

confidence: 99%

“…As a resource for the use of the Bge cell line, Wheeler et al (2018) sequenced the genome of one of the three Bge cell line subcultures available (Bge3). This effort addressed several key questions to answer were: how similar/dissimilar are the genomes of these Bge cells compared to the whole organism (B. glabrata)?…”

Section: Post-genome Advancesmentioning

confidence: 99%

Biomphalaria glabrata immunity: Post-genome advances

Castillo

Humphries

Mourão

et al. 2020

Developmental & Comparative Immunology

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“…The cell line originates from 5-day-old embryos of B. glabrata susceptible to infection with S. mansoni. The Bge cell line has been studied extensively to interrogate the host-parasite relationship because the Bge cell exhibits a hemocyte-like behavior that includes encapsulation of the larval parasite, but does not kill the parasites [20][21][22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Diminished adherence of Biomphalaria glabrata embryonic cell line to sporocysts of Schistosoma mansoni following programmed knockout of the allograft inflammatory factor

et al. 2020

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Background Larval development in an intermediate host gastropod snail of the genus Biomphalaria is an obligatory component of the life-cycle of Schistosoma mansoni. Understanding of the mechanism(s) of host defense may hasten the development of tools that block transmission of schistosomiasis. The allograft inflammatory factor 1, AIF, which is evolutionarily conserved and expressed in phagocytes, is a marker of macrophage activation in both mammals and invertebrates. AIF enhances cell proliferation and migration. The embryonic cell line, termed Bge, from Biomphalaria glabrata is a versatile resource for investigation of the snail-schistosome relationship since Bge exhibits a hemocyte-like phenotype. Hemocytes perform central roles in innate and cellular immunity in gastropods and in some cases can kill the parasite. However, the Bge cells do not kill the parasite in vitro. Methods Bge cells were transfected by electroporation with plasmid pCas-BgAIFx4, encoding the Cas9 nuclease and a guide RNA specific for exon 4 of the B. glabrata AIF (BgAIF) gene. Transcript levels for Cas9 and for BgAIF were monitored by reverse-transcription-PCR and, in parallel, adhesion of gene-edited Bge cells during co-culture with of schistosome sporocysts was assessed. Results Gene knockout manipulation induced gene-disrupting indels, frequently 1–2 bp insertions and/or 8–30 bp deletions, at the programmed target site; a range from 9 to 17% of the copies of the BgAIF gene in the Bge population of cells were mutated. Transcript levels for BgAIF were reduced by up to 73% (49.5 ± 20.2% SD, P ≤ 0.05, n = 12). Adherence by BgAIF gene-edited (ΔBgAIF) Bge to sporocysts diminished in comparison to wild type cells, although cell morphology did not change. Specifically, as scored by a semi-quantitative cell adherence index (CAI), fewer ΔBgAIF than control wild type cells adhered to sporocysts; control CAI, 2.66 ± 0.10, ΔBgAIF, 2.30 ± 0.22 (P ≤ 0.01). Conclusions The findings supported the hypothesis that BgAIF plays a role in the adherence of B. glabrata hemocytes to sporocysts during schistosome infection in vitro. This demonstration of the activity of programmed gene editing will enable functional genomics approaches using CRISPR/Cas9 to investigate additional components of the snail-schistosome host-parasite relationship.

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“…The cell line originates from ve-day-old embryos of B. glabrata susceptible to infection with S. mansoni. The Bge cell line has been studied extensively to interrogate the host-parasite relationship because the Bge cell exhibits a hemocyte-like behavior that includes encapsulation of the larval parasite [20][21][22][23][24][25][26][27][28]. The genome sequence of B. glabrata has been reported [29], along with ongoing transcriptome and proteome catalogues that include factors participating in immunological surveillance, phagocytosis, cytokine responses, and pathogen recognition receptor elements including Toll-like receptors and brinogen-related proteins [30][31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Diminished Adherence of Biomphalaria Glabrata Embryonic Cell Line to Sporocysts of Schistosoma Mansoni Following Programmed Knockout of the Allograft Inflammatory Factor

Coelho

Rodpai

Miller

et al. 2020

Preprint

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The development of the larval stage of Schistosoma mansoni in an intermediate host snail of the genus Biomphalaria is an obligatory component of the life cycle. Enhanced understanding of the mechanism(s) of host defense in the snail may hasten the development of tools that block transmission of schistosomiasis. The B. glabrata embryonic cell line, termed the Bge line, is a versatile resource for investigation of the snail-schistosome relationship. A key attribute of the Bge cell is the hemocyte-like phenotype, given the central role of the snail hemocyte in innate and cellular immunity. The allograft inflammatory factor 1, AIF, is evolutionarily conserved, typically is expressed in phagocytes and granular leukocytes, is a marker of macrophage activation in mammals and in invertebrates, and enhances cell proliferation and migration. AIF is highly expressed in strains of B. glabrata resistant S. mansoni infection in comparison with susceptible strains of the snail. We hypothesized that BgAIF may play a role in hemocyte proliferation, adhesion and/or migration after exposure of the snail to schistosomes. CRISPR/Cas gene knockout of the BgAIF gene in Bge cells was undertaken to investigate the hypothesis. Gene knockout manipulation induced gene-disrupting indels, frequently 1–2 bp insertions and/or 8–30 bp deletions, at the programmed target site; a range from 9 to 17% of BgAIF genes were mutated during 12 replicate experiments, and transcript levels for BgAIF were significantly reduced by up to 73% (range, 26 to 73%, mean 49.5 ± 20.2% S.D, n = 12) when monitored for up to nine days following the gene-editing manipulation. The adherence to sporocyst of BgAIF gene-edited (ΔBgAIF) Bge cells was significantly diminished in comparison to wild type cells, even though cell morphology did not differ between ΔBgAIF treatment and control groups of Bge cells. A Bge cell adherence index (CAI) to individual sporocysts was observed at 2.66 ± 0.10 for control and 2.30 ± 0.22 in ΔBgAIF cells (P < 0.05), revealing that ΔBgAIF cells were significantly less adherent than wild type Bge cells to primary sporocysts. The findings supported the hypothesis that BgAIF plays a role in the adherence of B. glabrata hemocytes to sporocysts during schistosome infection.

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Correction to: Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line

Cited by 5 publications

References 1 publication

Biomphalaria glabrata immunity: Post-genome advances

Biomphalaria glabrata immunity: Post-genome advances

Diminished adherence of Biomphalaria glabrata embryonic cell line to sporocysts of Schistosoma mansoni following programmed knockout of the allograft inflammatory factor

Diminished Adherence of Biomphalaria Glabrata Embryonic Cell Line to Sporocysts of Schistosoma Mansoni Following Programmed Knockout of the Allograft Inflammatory Factor

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