Correction to: Preliminary comparative analysis of the genomes of selected field reisolates of the Mycoplasma synoviae vaccine strain MS-H reveals both stable and unstable mutations after passage in vivo

Kordafshari, Somayeh; Shil, Pollob; Marenda, Marc S.; Olaogun, Olusola Martins; Konsak-Ilievski, Barbara; Disint, Jillian; Noormohammadi, Amir H.

doi:10.1186/s12864-020-07067-y

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“…High seropositivity rates were observed in the TS4 and AB1 inoculated chickens 3 weeks after inoculation. More importantly, the humoral responses of the in-contact chickens in groups exposed to the MS-H reisolates were consistent with the high systemic antibody responses to M. synoviae occasionally observed in a vaccinated flock (Kordafshari et al, 2020). Notably, the only group of in-contact chickens which developed more severe lesions were the group in-contact with TABLE 4 Reisolation of the M. synoviae strains from upper trachea (UT), lower trachea (LT), or air sacs (AS), 2-and 3-week after aerosol inoculation of SPF chickens with various strains or reisolates of M. synoviae following intratracheal inoculation with a virulent strain of IBV, and 2-and 3-week after exposure of in-contact chickens with the inoculated chickens.…”

Section: Discussionsupporting

confidence: 78%

“…High seropositivity rates were observed in the TS4 and AB1 inoculated chickens 3 weeks after inoculation. More importantly, the humoral responses of the in-contact chickens in groups exposed to the MS-H reisolates were consistent with the high systemic antibody responses to M. synoviae occasionally observed in a vaccinated flock ( Kordafshari et al, 2020 ). Notably, the only group of in-contact chickens which developed more severe lesions were the group in-contact with the 7NS inoculated chickens despite the low reisolation rate, serological response, and tracheal mucosal thickness in 7NS inoculated chickens.…”

Section: Discussionsupporting

confidence: 77%

“…Unusually high systemic antibody responses to M. synoviae in vaccinated flocks have been reported, and several MS-H derivatives have been isolated from these farms. The genomes of these reisolates occasionally carry reversions to the 7NS genotype in the genes obgE , oppF and gapdh , along with diverse repertoires of other sequence variations ( Kordafshari et al, 2020 ). These observations raised the question of the genetic, phenotypic, and immunogenic stability of MS-H in field conditions.…”

Section: Introductionmentioning

confidence: 99%

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Virulence factors of Mycoplasma synoviae: Three genes influencing colonization, immunogenicity, and transmissibility

Klose

Omotainse²,

Zare³

et al. 2022

Front. Microbiol.

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Infections caused by Mycoplasma synoviae are major welfare and economic concerns in poultry industries worldwide. These infections cause chronic respiratory disease and/or synovitis in chickens and turkeys leading to reduced production and increased mortality rates. The live attenuated vaccine strain MS-H (Vaxsafe® MS), commonly used for protection against M. synoviae infection in many countries, contains 32 single nucleotide variations compared to its wildtype parent strain, 86079/7NS. Genomic analysis of vaccine strains reisolated from flocks following the administration of MS-H has identified reversions to the original 86079/7NS sequence in the obgE, oppF and gapdh genes. Here, three MS-H field reisolates containing the 86079/7NS genotype in obgE (AS2), obgE and oppF (AB1), and obgE, oppF and gapdh (TS4), as well as the vaccine MS-H and the parental strain 86079/7NS were experimentally inoculated to chickens. The strains were assessed for their ability to infect and elicit immune responses in the recipient chickens, as well as in naïve in-contact chickens. Despite the loss of temperature sensitivity phenotype and colonization of the reisolates in the lower respiratory tract, there was no significant differences detected in the microscopic mucosal thickness of the middle or lower trachea of the inoculated chickens. Concurrent reversions in ObgE, OppF and GAPDH proteins were associated with higher gross air sac lesion scores and increased microscopic upper-tracheal mucosal thickness in chickens directly inoculated with the reisolates following intratracheal administration of a virulent strain of infectious bronchitis virus. The gross air sac lesions of the chickens in-contact with those inoculated with reisolates were not significantly different to those of chickens in-contact with MS-H inoculated chickens, suggesting that horizontal transmission of the reisolates in the poultry flock will not lead to higher pathogenicity or clinical signs. These results suggest a significant role of GAPDH and/or cumulative effect of ObgE, OppF and GAPDH on M. synoviae pathogenicity. Future experiments will be required to investigate the effect of single mutations in gapdh or oppF gene on pathogenicity of M. synoviae.

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Section: Discussionsupporting

confidence: 78%

“…High seropositivity rates were observed in the TS4 and AB1 inoculated chickens 3 weeks after inoculation. More importantly, the humoral responses of the in-contact chickens in groups exposed to the MS-H reisolates were consistent with the high systemic antibody responses to M. synoviae occasionally observed in a vaccinated flock ( Kordafshari et al, 2020 ). Notably, the only group of in-contact chickens which developed more severe lesions were the group in-contact with the 7NS inoculated chickens despite the low reisolation rate, serological response, and tracheal mucosal thickness in 7NS inoculated chickens.…”

Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Virulence factors of Mycoplasma synoviae: Three genes influencing colonization, immunogenicity, and transmissibility

Klose

Omotainse²,

Zare³

et al. 2022

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

Correction to: Preliminary comparative analysis of the genomes of selected field reisolates of the Mycoplasma synoviae vaccine strain MS-H reveals both stable and unstable mutations after passage in vivo

Abstract: An amendment to this paper has been published and can be accessed via the original article.

Cited by 1 publication

References 1 publication

Virulence factors of Mycoplasma synoviae: Three genes influencing colonization, immunogenicity, and transmissibility

Virulence factors of Mycoplasma synoviae: Three genes influencing colonization, immunogenicity, and transmissibility

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