Correction to: Physicochemical properties of polysaccharides from Dendrobium officinale by fractional precipitation and their preliminary antioxidant and anti-HepG2 cells activities in vitro

Xing, Shangping; Zhang, Xiaofeng; Ke, Hannu; Jiang, Lin; Huang, Yuechun; Wei, Gang

doi:10.1186/s13065-018-0471-9

Cited by 5 publications

(6 citation statements)

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“…Moreover, we speculated that the molecular weight of DOPA-1 and DCPP1a-1 may decrease closer to the lower limit of our suitable molecular weight range (28.48 kDa) after degradation by the gastrointestinal in vivo. e polysaccharide DOP-70 (Mw: 50.3 kDa, within our suitable molecular weight range) from Dendrobium officinale, obtained by our group before [17], also exhibited a better anti-HepG2 cell activity in vitro. In addition, through preexperiments, we have investigated that the antitumor activity of DOPs isolated by our group before (Mw: 776 kDa) [32] and monosaccharide (Mw < 10 kDa), whose molecular weight was all outside our suitable molecular weight range, was not significant.…”

Section: Discussionmentioning

confidence: 86%

“…Zhao et al degraded polysaccharides from Porphyra haitanensis using the H 2 O 2 system to obtain fragments of different molecular weights and proved that the antioxidant activity of them was closely related to the molecular weight [16]. However, in terms of DOPs, some studies applied polysaccharides by fractional precipitation or the total polysaccharides to investigate the antitumor activity [17,18], and fewer reports have published concerning polysaccharides that degraded into a series of molecular weights but lacking comparison of molecular weights [18].…”

Section: Introductionmentioning

confidence: 99%

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Physicochemical and Antioxidant Properties of the Degradations of Polysaccharides from Dendrobium officinale and Their Suitable Molecular Weight Range on Inducing HeLa Cell Apoptosis

Zhang

Luo

Wei

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Different molecular weight polysaccharides of Dendrobium officinale (DOPs) have gradually attracted attention because of their broad biological activities. They, however, remain poorly defined whether their antitumor activity is associated with molecular weight. In this study, the physicochemical, antioxidant, and antitumor properties of DOPs, including the crude polysaccharide (DOP) and its six degradation fractions (DOP1–DOP6) extracted from Dendrobium officinale, were determined. Consequently, DOPs were mainly composed of different ratios of mannose and glucose as follows: 5.15 : 1, 4.62 : 1, 4.19 : 1, 4.46 : 1, 4.32 : 1, 4.29 : 1, and 4.23 : 1, and their molecular weights were significantly different ranging from 652.29 kDa to 11.10 kDa. With the concentration increase of DOPs, the scavenging capacity against OH and DPPH free radicals increased. The antitumor ability of DOPs was different that DOP1–DOP5 (Mw: 176.29 kDa–28.48 kDa) exhibited the best antiproliferation activity than DOP (Mw: 652.29 kDa) and DOP6 (Mw: 11.10 kDa) in HeLa cells rather than PC9, A549, and HepG2 cells. Moreover, it is worth mentioning that DOP1 and DOP5 showed stronger capability on inducing apoptosis of HeLa cells than DOP and DOP6 via the mitochondrial pathway by upregulating the ratio of the Bax/Bal-2 mRNA expression. The results demonstrated that DOPs can be used as the potential natural antioxidant and antitumor products in pharmaceutical industries, and the molecular weight is a crucial influential factor of their antitumor activity that 28.48 kDa–176.29 kDa is a suitable range we may refer to.

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Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Physicochemical and Antioxidant Properties of the Degradations of Polysaccharides from Dendrobium officinale and Their Suitable Molecular Weight Range on Inducing HeLa Cell Apoptosis

Zhang

Luo

Wei

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Cells were seeded in 6-well culture plates (3×10 5 cells/well) and were untreated or co-treated with EGF (20 ng/ml)/TGF-β1 (10 ng/ml) and Tan IIA (2 µM)/LY294002 (20 µM)/U0126 (20 µM) for 48 h at 37°C. The total protein was extracted using RIPA buffer mixed with 1% PMSF (Beyotime Institute of Biotechnology), and the protein concentration was analyzed using a bicinchoninic acid protein assay kit (Nanjing KeyGen Biotech Co., Ltd.), as previously described (29). Equal amounts of total protein (30 µg) samples were separated by SDS-PAGE (8–12% gel) and transferred onto PVDF membranes (EMD Millipore).…”

Section: Methodsmentioning

confidence: 99%

Tanshinone IIA reverses EGF‑ and TGF‑β1‑mediated epithelial‑mesenchymal transition in HepG2 cells via the PI3K/Akt/ERK signaling pathway

Zhang¹,

Lin²,

Chen³

et al. 2019

Oncol Lett

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Epithelial-to-mesenchymal transition (EMT) is an essential phenotypic conversion involved in cancer progression. Epidermal growth factor (EGF) and transforming growth factor (TGF)-β1 are potent inducers of the EMT. Tanshinone IIA (Tan IIA) is a phenanthrenequinone extracted from the root of Salvia miltiorrhiza Bunge, and its anticancer activity has been demonstrated in numerous studies. However, the mechanisms of action underlying Tan IIA in EGF-and TGF-β1-induced EMT in HepG2 cells remain unknown. Multiple assays were utilized in the present study, including colony formation, wound healing, Transwell invasion, immunofluorescence staining and western blotting, in order to assess the influence of Tan IIA on HepG2 cells induced by 20 ng/ml EGF and 10 ng/ml TGF-β1. The present study reported that Tan IIA treatment decreased EGF-and TGF-β1-enhanced cell colony numbers, migration and invasion, and inhibited EGF-and TGF-β1-induced decreases in the expression levels of E-cadherin, and increases in the expression levels of matrix metalloproteinase-2, N-cadherin, vimentin and Snail. In addition, it was observed that Tan IIA decreased the expression levels of phosphorylated (p)-Akt and p-ERK1/2 induced by EGF and TGF-β1. Furthermore, western blot analysis confirmed that blocking the function of PI3K/Akt and ERK with LY294002 and U0126 resulted in upregulation of E-cadherin expression, and downregulation of vimentin and Snail expression in EGF-and TGF-β1-treated HepG2 cells. In conclusion, to the best of our knowledge, the results of the present study are the first to indicate that Tan IIA may suppress EGF-and TGF-β1-induced EMT in HepG2 cells by deactivating the PI3K/Akt/ERK pathway.

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“…The cytotoxicity of PJPS was calculated according to a method reported previously (Xing et al, 2018). Human ovarian carcinoma cells (Skov3 and A2780) were cultured in DME/F12 medium (Hyclone, Logan, UT, USA), together with supplemented with fetal bovine serum (10%) and antibiotics (100 U/mL penicillin and 100 µg/mL streptomycin) at 37°C as well as 5% CO 2 in a humidified incubator.…”

Section: Cytotoxicity Of Pjps and Cm-pjpsmentioning

confidence: 99%

Extraction, preparation and an assessment of the activity of carboxymethyl polysaccharide from Panax japonicus

Yu¹,

Yuan²,

Yan³

et al. 2022

Food Sci. Technol

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A response surface method (RSM) was employed to optimize polysaccharide extraction from Panax japonicus (PJPS). Also, carboxymethyl substitution of PJPS (CM-PJPS) was studied using a response surface methodology. A three-variable Box-Behnken design (BBD) methodology was applied. RSM analyses revealed that conditions that maximized polysaccharide yield were as follows: a liquid-to-material ratio 22:1, an extraction temperature of 83.99 °C and an extraction duration of 2.32 h. Under optimum extraction conditions, the average absorbance value of PJPS was 0.6001, and the purity of PJPS was 98.2%. A quadratic regression model for CM-PJPS was obtained via BBD. The optimum extraction conditions for the process were determined to include a 1.38 h reaction time, 1.24 g monochloroacetic acid (MCA) and a reaction temperature of 52.85 °C. Under optimum extraction conditions, the average degree of CM-PJPS substitution was 0.9733. The structure of the PJPS was examined by means of Fourier transform infrared spectroscopy (FTIR). CM-PJPS exhibited the strongest anti-oxidant activity in vitro. In addition, CM-PJPS inhibited Skov3 and A2780 cancer cell proliferation. Our data revealed that CM-PJPS is a promising natural antioxidant with potential value as a food supplement and for treatment of cancer.

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Correction to: Physicochemical properties of polysaccharides from Dendrobium officinale by fractional precipitation and their preliminary antioxidant and anti-HepG2 cells activities in vitro

Cited by 5 publications

References 1 publication

Physicochemical and Antioxidant Properties of the Degradations of Polysaccharides from Dendrobium officinale and Their Suitable Molecular Weight Range on Inducing HeLa Cell Apoptosis

Physicochemical and Antioxidant Properties of the Degradations of Polysaccharides from Dendrobium officinale and Their Suitable Molecular Weight Range on Inducing HeLa Cell Apoptosis

Tanshinone IIA reverses EGF‑ and TGF‑β1‑mediated epithelial‑mesenchymal transition in HepG2 cells via the PI3K/Akt/ERK signaling pathway

Extraction, preparation and an assessment of the activity of carboxymethyl polysaccharide from Panax japonicus

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