Correction to: Phylogenetics and antibacterial properties of exopolysaccharides from marine bacteria isolated from Mauritius seawater

Aullybux, Aadil Ahmad; Puchooa, Daneshwar; Bahorun, Theeshan; Jeewon, Rajesh

doi:10.1007/s13213-019-01495-2

Cited by 4 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aullybux et al 47 tested the antibacterial activity of EPS (extracted from Halomonas sp. ) which exhibited promising antibacterial properties against several human pathogens namely Enterobacter aerogenes , Escherichia coli , Proteus vulgaris and Staphylococcus aureus .…”

Section: Resultsmentioning

confidence: 99%

Selenium nanoparticles coated bacterial polysaccharide with potent antimicrobial and anti-lung cancer activities

Shehata,

Elwakil,

Elshewemi

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Bacterial exopolysaccharides are homopolymeric or heteropolymeric polysaccharides with large molecular weights (10–1000 kDa). Exopolysaccharides' functional uses and potential have revolutionized the industrial and medicinal industries. Hence, the aim of the present study was to optimize the production of bacterial exopolysaccharide and apply it as a capping agent for selenium nanoparticles synthesis. Exopolysaccharide (EPS) producing Lactic acid bacteria (LAB) were isolated from dairy products then biochemically characterized and assessed for their potential antimicrobial effect. The most potent EPS producer was identified as Lactiplantibacillus plantarum strain A2 with accession number OP218384 using 16S rRNA sequencing. Overall, FTIR data of the extracted EPS revealed similarity with amylopectin spectrum. 1H NMR spectrum revealed an α-anomeric configuration of the glycosidic linkage pattern in the polysaccharides while the 13C NMR spectrum can also be separated into two main portions, the anomeric carbons region (δ 98–102 ppm) and the non-anomeric carbons region (δ 60–81 ppm). Antimicrobial activity of the produced EPS showed maximum activity against Staphylococcus aureus, MRSA, Enterobacter aerogenes, Klebsiella pneumoniae and Candida albicans respectively. The EPS capsule layer surrounding the bacterial cells was detected by TEM study. Optimization of EPS production was evaluated using Taguchi design, trial 23 reported the highest biomass yield and EPS output (6.5 and 27.12 g/L respectively) with 2.4 and 3.3 folds increase (from the basal media) respectively. The optimized exopolysaccharide was used as a capping and stabilizing agent for selenium nanoparticles (EPS-SeNPs) synthesis. Zeta potential, size and PDI of the synthesized nanoparticles were − 19.7 mV, 45–65 nm and 0.446 respectively with strong bactericidal and fungicidal effect against the tested pathogens. Complete microbial growth eradication was recorded after 6, 8 and 10 h against Staphylococcus aureus, Candida albicans and Klebsiella pneumoniae respectively. EPS-SeNPs showed a potent antioxidant effect reached 97.4% and anticancer effect against A549 lung cancer cell line (IC50 reached 5.324 µg/mL). EPS-SeNPs inhibited cancerous cell growth at S phase. Moreover, molecular studies revealed the anti-apoptotic activity of Bcl2's was inhibited and Bax was activated. The present investigation successfully synthesized selenium nanoparticles through bacterial EPS with significantly high antimicrobial and anticancer activity.

show abstract

Section: Resultsmentioning

confidence: 99%

Selenium nanoparticles coated bacterial polysaccharide with potent antimicrobial and anti-lung cancer activities

Shehata,

Elwakil,

Elshewemi

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The interest in EPSs, to a greater extent, is conditioned by their amphiphilic nature and ability to interact with bacterial membranes, leading to the alteration of glycoproteins and lipids on the cytomembrane of microorganisms, thus affecting ion exchange and reducing ATF synthesis. This statement can be reinforced by an observation made by Goy et al [23] for chitosan and, recently, by Aullybux et al [24] for EPSs isolated from marine bacteria Mauritius seawater. Antimicrobial and antibiofilm efficiency of bacterial cellulose has been constantly documented [25,26].…”

Section: Introductionmentioning

confidence: 81%

Elaboration of Nanostructured Levan-Based Colloid System as a Biological Alternative with Antimicrobial Activity for Applications in the Management of Pathogenic Microorganisms

Radenkovs,

Valdovska,

Galina

et al. 2023

Nanomaterials

View full text Add to dashboard Cite

Considering the documented health benefits of bacterial exopolysaccharides (EPSs), specifically of bacterial levan (BL), including its intrinsic antimicrobial activity against certain pathogenic species, the current study concentrated on the development of active pharmaceutical ingredients (APIs) in the form of colloid systems (CoSs) containing silver nanoparticles (AgNPs) employing in-house biosynthesized BL as a reducing and capping agent. The established protocol of fermentation conditions implicating two species of lactic acid bacteria (LAB), i.e., Streptococcus salivarius K12 and Leuconostoc mesenteroides DSM 20343, ensured a yield of up to 25.7 and 13.7 g L−1 of BL within 72 h, respectively. An analytical approach accomplished by Fourier-transform infrared (FT-IR) spectroscopy allowed for the verification of structural features attributed to biosynthesized BL. Furthermore, scanning electron microscopy (SEM) revealed the crystalline morphology of biosynthesized BL with a smooth and glossy surface and highly porous structure. Molecular weight (Mw) estimated by multi-detector size-exclusion chromatography (SEC) indicated that BL biosynthesized using S. salivarius K12 has an impressively high Mw, corresponding to 15.435 × 104 kilodaltons (kDa). In turn, BL isolated from L. mesenteroides DSM 20343 was found to have an Mw of only 26.6 kDa. Polydispersity index estimation (PD = Mw/Mn) of produced BL displayed a monodispersed molecule isolated from S. salivarius K12, corresponding to 1.08, while this was 2.17 for L. mesenteroides DSM 20343 isolate. The presence of fructose as the main backbone and, to a lesser extent, glucose and galactose as side chain molecules in EPS hydrolysates was supported by HPLC-RID detection. In producing CoS-BL@AgNPs within green biosynthesis, the presence of nanostructured objects with a size distribution from 12.67 ± 5.56 nm to 46.97 ± 20.23 was confirmed by SEM and energy-dispersive X-ray spectroscopy (EDX). The prominent inhibitory potency of elaborated CoS-BL@AgNPs against both reference test cultures, i.e., Pseudomonas aeruginosa, Escherichia coli, Enterobacter aerogenes, and Staphylococcus aureus and those of clinical origin with multi-drug resistance (MDR), was confirmed by disc and well diffusion tests and supported by the values of the minimum inhibitory and bactericidal concentrations. CoS-BL@AgNPs can be treated as APIs suitable for designing new antimicrobial agents and modifying therapies in controlling MDR pathogens.

show abstract

“…[34][35][36] . They have successfully inhibited the bacterial growth of a plethora of human pathogenic bacteria belongingto Acinetobacter, Bacillus, Campylobacter, Enterobacter, Enterococcus, Escherichia, Proteus, Pseudomonas, Salmonella, Streptococcus and Staphylococcus genera with more susceptibility of gram-negative bacteria than the gram-positive ones 37,38 .…”

Section: Discussionmentioning

confidence: 99%

Isolation, Molecular Identification and Antibacterial Potential of Marine Bacteria from Deep Atlantic Ocean of Morocco

Chbel¹,

Rodríguez-Castro²,

Quinteiro³

et al. 2022

AJMB

View full text Add to dashboard Cite

Background: Antibiotic resistance is an important concern for the public health authorities at global level. It is detrimental to human and environmental ecosystems, thus, there is a big need for natural bioactive compounds. In this work, we aimed to find out biomolecules derived from marine bacteria that may constitute an alternative to antibiotics. Methods: We isolated and identified thirty one marine bacteria collected from deep ocean water in central coast of Safi city, Morocco. Then, we induced biomolecules production in six marine bacterial strains. The extracts were tested for their antibacterial activity against gram-negative and gram-positive bacteria such as Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 33592 and Listeria monocytogenes ATCC 19117. Furthermore, we partially analyzed the chemical composition of these biomolecules and evaluated their sensibility to different temperatures. Results: The six marine bacteria were able to produce molecules which inhibited the three pathogenic strains with high inhibition zones reaching 27 mm. These molecules were characterized by heat stability from 60 to 121°C relying on each strain. Conclusion: The produced molecules may offer a great potential to pharmaceutical industries as they may constitute an alternative to antibiotics that are becoming less effective due to the emergence of drugs resistance.

show abstract

Correction to: Phylogenetics and antibacterial properties of exopolysaccharides from marine bacteria isolated from Mauritius seawater

Cited by 4 publications

References 0 publications

Selenium nanoparticles coated bacterial polysaccharide with potent antimicrobial and anti-lung cancer activities

Selenium nanoparticles coated bacterial polysaccharide with potent antimicrobial and anti-lung cancer activities

Elaboration of Nanostructured Levan-Based Colloid System as a Biological Alternative with Antimicrobial Activity for Applications in the Management of Pathogenic Microorganisms

Isolation, Molecular Identification and Antibacterial Potential of Marine Bacteria from Deep Atlantic Ocean of Morocco

Contact Info

Product

Resources

About